Molecular characterization and expression analysis of nine cotton GhEF1A genes encoding translation elongation factor 1A

Xu, Wen‐Liang; Wang, Xiulan; Wang, Hong; Li, Xuebao

doi:10.1016/j.gene.2006.09.014

Cited by 27 publications

(18 citation statements)

References 44 publications

(44 reference statements)

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“…Indeed, abundance of the proteins identified in nodules directly subjected to drought (PDD) showed a significant decline compared to PDC (Table 1). This is the case of elongation factor 1-alpha, which promotes the binding of aminoacyl-tRNA to the ribosome during protein biosynthesis and whose expression has been correlated to high rates of protein synthesis in developing plant tissues (Xu et al ., 2007). …”

Section: Discussionmentioning

confidence: 99%

Local inhibition of nitrogen fixation and nodule metabolism in drought-stressed soybean

Gil‐Quintana

Larrainzar

Seminario

et al. 2013

Journal of Experimental Botany

107

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Drought stress is a major factor limiting symbiotic nitrogen fixation (NF) in soybean crop production. However, the regulatory mechanisms involved in this inhibition are still controversial. Soybean plants were symbiotically grown in a split-root system (SRS), which allowed for half of the root system to be irrigated at field capacity while the other half remained water deprived. NF declined in the water-deprived root system while nitrogenase activity was maintained at control values in the well-watered half. Concomitantly, amino acids and ureides accumulated in the water-deprived belowground organs regardless of transpiration rates. Ureide accumulation was found to be related to the decline in their degradation activities rather than increased biosynthesis. Finally, proteomic analysis suggests that plant carbon metabolism, protein synthesis, amino acid metabolism, and cell growth are among the processes most altered in soybean nodules under drought stress. Results presented here support the hypothesis of a local regulation of NF taking place in soybean and downplay the role of ureides in the inhibition of NF.

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Section: Discussionmentioning

confidence: 99%

Local inhibition of nitrogen fixation and nodule metabolism in drought-stressed soybean

Gil‐Quintana

Larrainzar

Seminario

et al. 2013

Journal of Experimental Botany

107

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“…However, salt and heavy metal induced the accumulation of rice eIF5A-1 and eIF5A-2 mRNAs in rice cells [82]. Elongation factors whose expressions have been demonstrated to correlate to the high rate of protein synthesis in developing plant tissues [83] facilitate translational elongation, from the formation of the first peptide bond to the formation of the last one in the ribosome. Guo et al [84] isolated a translation elongation factor 2-like protein gene from a cold defective Arabidopsis mutant, los 1–1 .…”

Section: Discussionmentioning

confidence: 99%

Leaf cDNA-AFLP analysis of two citrus species differing in manganese tolerance in response to long-term manganese-toxicity

Zhou

You

et al. 2013

BMC Genomics

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BackgroundVery little is known about manganese (Mn)-toxicity-responsive genes in citrus plants. Seedlings of ‘Xuegan’ (Citrus sinensis) and ‘Sour pummelo’ (Citrus grandis) were irrigated for 17 weeks with nutrient solution containing 2 μM (control) or 600 μM (Mn-toxicity) MnSO4. The objectives of this study were to understand the mechanisms of citrus Mn-tolerance and to identify differentially expressed genes, which might be involved in Mn-tolerance.ResultsUnder Mn-toxicity, the majority of Mn in seedlings was retained in the roots; C. sinensis seedlings accumulated more Mn in roots and less Mn in shoots (leaves) than C. grandis ones and Mn concentration was lower in Mn-toxicity C. sinensis leaves compared to Mn-toxicity C. grandis ones. Mn-toxicity affected C. grandis seedling growth, leaf CO2 assimilation, total soluble concentration, phosphorus (P) and magenisum (Mg) more than C. sinensis. Using cDNA-AFLP, we isolated 42 up-regulated and 80 down-regulated genes in Mn-toxicity C. grandis leaves. They were grouped into the following functional categories: biological regulation and signal transduction, carbohydrate and energy metabolism, nucleic acid metabolism, protein metabolism, lipid metabolism, cell wall metabolism, stress responses and cell transport. However, only 7 up-regulated and 8 down-regulated genes were identified in Mn-toxicity C. sinensis ones. The responses of C. grandis leaves to Mn-toxicity might include following several aspects: (1) accelerating leaf senescence; (2) activating the metabolic pathway related to ATPase synthesis and reducing power production; (3) decreasing cell transport; (4) inhibiting protein and nucleic acid metabolisms; (5) impairing the formation of cell wall; and (6) triggering multiple signal transduction pathways. We also identified many new Mn-toxicity-responsive genes involved in biological and signal transduction, carbohydrate and protein metabolisms, stress responses and cell transport.ConclusionsOur results demonstrated that C. sinensis was more tolerant to Mn-toxicity than C. grandis, and that Mn-toxicity affected gene expression far less in C. sinensis leaves. This might be associated with more Mn accumulation in roots and less Mn accumulation in leaves of Mn-toxicity C. sinensis seedlings than those of C. grandis seedlings. Our findings increase our understanding of the molecular mechanisms involved in the responses of plants to Mn-toxicity.

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“…The ESTs were assembled into 5852 unigenes and annotated through a similarity search. Annotation results showed that many previously reported cotton fiber active or key genes were included in these libraries, such as AGPs and FLAs [42]–[44], GhTUB, GhTUA [15], [17], actin-related genes (including actin-depolymerizing factor and profilin ) [14], [16], [18], [45]–[47], and GhEF1As [48]. A normalized cDNA library was an efficient tool for gene identification because it reduced the frequencies of prevalent mRNAs while enriching the rare ones.…”

Section: Discussionmentioning

confidence: 99%

Generation, Annotation and Analysis of First Large-Scale Expressed Sequence Tags from Developing Fiber of Gossypium barbadense L

Yuan

Zhang

2011

PLoS ONE

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BackgroundCotton fiber is the world's leading natural fiber used in the manufacture of textiles. Gossypium is also the model plant in the study of polyploidization, evolution, cell elongation, cell wall development, and cellulose biosynthesis. G. barbadense L. is an ideal candidate for providing new genetic variations useful to improve fiber quality for its superior properties. However, little is known about fiber development mechanisms of G. barbadense and only a few molecular resources are available in GenBank.Methodology and Principal FindingsIn total, 10,979 high-quality expressed sequence tags (ESTs) were generated from a normalized fiber cDNA library of G. barbadense. The ESTs were clustered and assembled into 5852 unigenes, consisting of 1492 contigs and 4360 singletons. The blastx result showed 2165 unigenes with significant similarity to known genes and 2687 unigenes with significant similarity to genes of predicted proteins. Functional classification revealed that unigenes were abundant in the functions of binding, catalytic activity, and metabolic pathways of carbohydrate, amino acid, energy, and lipids. The function motif/domain-related cytoskeleton and redox homeostasis were enriched. Among the 5852 unigenes, 282 and 736 unigenes were identified as potential cell wall biosynthesis and transcription factors, respectively. Furthermore, the relationships among cotton species or between cotton and other model plant systems were analyzed. Some putative species-specific unigenes of G. barbadense were highlighted.Conclusions/SignificanceThe ESTs generated in this study are from the first large-scale EST project for G. barbadense and significantly enhance the number of G. barbadense ESTs in public databases. This knowledge will contribute to cotton improvements by studying fiber development mechanisms of G. barbadense, establishing a breeding program using marker-assisted selection, and discovering candidate genes related to important agronomic traits of cotton through oligonucleotide array. Our work will also provide important resources for comparative genomics, polyploidization, and genome evolution among Gossypium species.

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Molecular characterization and expression analysis of nine cotton GhEF1A genes encoding translation elongation factor 1A

Cited by 27 publications

References 44 publications

Local inhibition of nitrogen fixation and nodule metabolism in drought-stressed soybean

Local inhibition of nitrogen fixation and nodule metabolism in drought-stressed soybean

Leaf cDNA-AFLP analysis of two citrus species differing in manganese tolerance in response to long-term manganese-toxicity

Generation, Annotation and Analysis of First Large-Scale Expressed Sequence Tags from Developing Fiber of Gossypium barbadense L

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