Molecular characterization and expression analysis of interferon-gamma in the large yellow croaker Larimichthys crocea

Chen, Ruan-Ni; Su, Yongquan; Wang, Jun; Liu, Min; Qiao, Ying; Mao, Yong; Ke, Qiaozhen; Han, Kunhuang; Zheng, Weiqiang; Zhang, Jianshe; Wu, Changwen

doi:10.1016/j.fsi.2015.07.008

Cited by 25 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Aeromonas salmonicida infection induces an increased IFNγ transcription in the intestine of Atlantic salmon 213 . Moreover, large yellow croaker ( Larimichthys crocea ) infected with Vibrio harveyi exhibited a higher expression of IFNγ on the first day of infection in several tissues 214 . In turbot ( Scophthalmus maximus ), upregulation of TNFα transcription in the intestine was observed upon the administration of probiotic lactic acid bacteria 215 .…”

Section: Crosstalk Between Commensal Bacteria and Mucosal Adaptive Immunitymentioning

confidence: 99%

Commensal bacteria‐immunity crosstalk shapes mucosal homeostasis in teleost fish

Ding

Huang

et al. 2021

Reviews in Aquaculture

View full text Add to dashboard Cite

Aquaculture is a fast-growing and rapidly expanding industry worldwide, which currently represents an important food source for the human population, providing more than 20% of the world's animal protein requirements and maintaining an accelerated growth rate of 18% per year. 1,2 During the past decades, aquaculture systems have gradually shifted from extensive to intensive, with stocking densities

show abstract

Section: Crosstalk Between Commensal Bacteria and Mucosal Adaptive Immunitymentioning

confidence: 99%

Commensal bacteria‐immunity crosstalk shapes mucosal homeostasis in teleost fish

Ding

Huang

et al. 2021

Reviews in Aquaculture

View full text Add to dashboard Cite

show abstract

“…Also, the contents of mucus IgM and complement C3, and the protective immune rate are different [ 7 – 9 ]. In recent years, numerous data have been produced regarding gene-level regulation that suggests that immune-related (IR) factors such as TLR2 , MyD88 , IL1β [ 10 ], Pc-pis [ 11 ], TNF-α , MHC I/II , and TGFβ1 [ 12 ], NCCRP1 [ 13 ], TRAF6 [ 14 ], IRAK4 [ 15 ], IL34/MCSF2 , MCSFR1/MCSFR2 [ 16 ], Nrdp1 [ 17 ] and Interferon-γ [ 18 ] in the fish are linked to C. irritans infection. Despite this work, the changes in the relative amounts of a few factors are not enough to provide a thorough understanding of the immune responses in infected fish.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic analysis reveals the key immune-related signalling pathways of Sebastiscus marmoratus in response to infection with the parasitic ciliate Cryptocaryon irritans

Yin

Dong

2017

Parasites Vectors

View full text Add to dashboard Cite

BackgroundFalse kelpfish (Sebastiscus marmoratus) is one of the target species in artificial breeding in China, and is susceptible to infection by Cryptocaryon irritans, which is an obligate parasitic ciliate that lives in the epithelium of the fish gills, skin and fins. Here, we sought to understand the mechanisms of molecular immunity of S. marmoratus against C. irritans infection.MethodsWe carried out an extensive analysis of the transcriptome of S. marmoratus immune-related tissues. A paired-end library was constructed from the cDNA synthesized using a Genomic Sample Prep Kit. Five normalized cDNA libraries were constructed using RNA from the control group and the four groups of C. irritans-infected fish. The libraries were sequenced on an Illumina Mi-Seq platform, and functional annotation of the transcriptome was performed using bioinformatics software.ResultsThe data produced a total of 149,983,397 clean reads from five cDNA libraries constructed from S. marmoratus immune-related tissues. A total of 33,291 unigenes were assembled with an average length of 1768 bp. In eggNOG (Evolutionary Genealogy of Genes: non-supervised orthologous groups) categories, 333 unigenes (0.94%) were assigned to defense mechanisms. In the immune system process sub-categories of gene ontology (GO) enrichment analysis, with the passage of time post-infection, the number of differentially expressed genes (DEGs) was reduced from 24 h to 48 h but then increased from 72 h to 96 h. Specifically, the immune-related differentially expressed genes (IRDEGs), which belong to the KEGG (Kyoto encyclopedia of genes and genomes) pathways, such as the complement and coagulation cascades, chemokine signalling pathways and toll-like receptor signalling pathways were mainly observed at 24 h post-infection.ConclusionsInfection with C. irritans resulted in a large number of DEGs in the immune-related tissues of S. marmoratus. The rapid and significant response of the S. marmoratus immune signalling pathways following C. irritans infection may be associated with their involvement in the immune process.Electronic supplementary materialThe online version of this article (10.1186/s13071-017-2508-7) contains supplementary material, which is available to authorized users.

show abstract

“…From the EST sequences, 87 immune-related genes were selected for further analysis (Table S5). These genes were related to interferon, interleukin, tumor necrosis factor, chemokine, toll-like receptor and complement component which play pivotal roles in resisting the bacterial invasion [37,38,39,40]. A majority of these genes were targeted by multiple miRNAs at different sites, but several of them were targeted by only one miRNA.…”

Section: Resultsmentioning

confidence: 99%

Identification and Characterization of MicroRNAs in the Liver of Blunt Snout Bream (Megalobrama amblycephala) Infected by Aeromonas hydrophila

Cui

Wei

et al. 2016

IJMS

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are small RNA molecules that play key roles in regulation of various biological processes. In order to better understand the biological significance of miRNAs in the context of Aeromonas hydrophila infection in Megalobrama amblycephala, small RNA libraries obtained from fish liver at 0 (non-infection), 4, and 24 h post infection (poi) were sequenced using Illumina deep sequencing technology. A total of 11,244,207, 9,212,958, and 7,939,157 clean reads were obtained from these three RNA libraries, respectively. Bioinformatics analysis identified 171 conserved miRNAs and 62 putative novel miRNAs. The existence of ten randomly selected novel miRNAs was validated by RT-PCR. Pairwise comparison suggested that 61 and 44 miRNAs were differentially expressed at 4 and 24 h poi, respectively. Furthermore, the expression profiles of nine randomly selected miRNAs were validated by qRT-PCR. MicroRNA target prediction, gene ontology (GO) annotation, and Kyoto Encylopedia of Genes and Genomes (KEGG) analysis indicated that a variety of biological pathways could be affected by A. hydrophila infection. Additionally, transferrin (TF) and transferrin receptor (TFR) genes were confirmed to be direct targets of miR-375. These results will expand our knowledge of the role of miRNAs in the immune response of M. amblycephala to A. hydrophila infection, and facilitate the development of effective strategies against A. hydrophila infection in M. amblycephala.

show abstract

Molecular characterization and expression analysis of interferon-gamma in the large yellow croaker Larimichthys crocea

Cited by 25 publications

References 32 publications

Commensal bacteria‐immunity crosstalk shapes mucosal homeostasis in teleost fish

Commensal bacteria‐immunity crosstalk shapes mucosal homeostasis in teleost fish

Transcriptomic analysis reveals the key immune-related signalling pathways of Sebastiscus marmoratus in response to infection with the parasitic ciliate Cryptocaryon irritans

Identification and Characterization of MicroRNAs in the Liver of Blunt Snout Bream (Megalobrama amblycephala) Infected by Aeromonas hydrophila

Contact Info

Product

Resources

About