Molecular characterization and expression patterns of insulin-like growth factor-binding protein genes in postnatal Nanjiang brown goats

Sy, Zhan; Chen, L; L, Li; Lj, Wang; Zhong, Tao; Zhang, Hp

doi:10.4238/2015.october.16.22

Cited by 6 publications

(5 citation statements)

References 46 publications

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“…The Bos taurus was closely related to Bubalus bubalis, whereas Capra hircus and Ovis aries shared higher sequence similarities. Our results are also supported by the study performed in Capra hircus, where Ovis aries and Capra hircus were closely related evolutionary, whereas Bos taurus was distantly related [35].…”

Section: Evolutionary and Phylogenetic Analysissupporting

confidence: 89%

Comparative Genomic Characterization of Insulin-Like Growth Factor Binding Proteins in Cattle and Buffalo

Saif-ur-Rehman

Mushtaq

Hassan

et al. 2022

BioMed Research International

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The somatotropic axis consists of genes associated with economic traits like muscle growth and carcass traits in livestock. Insulin-like growth factor binding proteins (IGFBPs) are the major proteins that play a vital role in the somatotropic axis. The present study performed a genome-wide characterization of IGFBP genes in cattle. Genomic sequences of the IGFBP gene family for different mammals (cattle, buffalo, goat, and sheep) were recovered from the NCBI database. Sequence analyses were performed to investigate cattle’s genomic variations in the IGFBP gene family. Phylogenetic analysis, gene structure, motif patterns, and conserved domain analysis (CDA) of the IGFBP family revealed the evolutionarily conserved nature of the IGFBP genes in cattle and other studied species. Physicochemical properties of IGFBP proteins in cattle revealed that most of these proteins are unstable, hydrophilic, thermostable, and acidic. Comparative amino acid analysis revealed variations in all protein sequences with single indels in IGFBP3 and IGFBP6. Mutation analysis revealed only one nonsynonymous mutation D212 > E in the IGFBP6 protein of cattle. A total of 245 nuclear hormone receptor (NHRs) sites were detected, including 139 direct repeats (DR), 65 everted repeats (ER), and 41 inverted repeats (IR). Out of 133 transcription factors (TFs), 10 TFs (AHR, AHRARNT, AP4, CMYB, E47, EGR2, GATA, SP1, and SRF) had differential distribution ( P value < 0.05) of putative transcriptional binding sites (TFBS) in cattle compared to buffalo. Synteny analysis revealed the conserved nature of genes between cattle and buffalo. Two gene pairs (IGFBP1/IGFBP3 and IGFBP2/IGFBP5) showed tandem duplication events in cattle and buffalo. This study highlights the functional importance of genomic variation in IGFBP genes and necessitates further investigations better to understand the role and mechanisms of IGFBPs in bovines.

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Section: Evolutionary and Phylogenetic Analysissupporting

confidence: 89%

Comparative Genomic Characterization of Insulin-Like Growth Factor Binding Proteins in Cattle and Buffalo

Saif-ur-Rehman

Mushtaq

Hassan

et al. 2022

BioMed Research International

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“…Eight SNPs have been detected in exon 2 of IGFBP-3 gene, 6 of them produce nonsynonymous protein coding or non-synonymous mutation (Sharma et al 2014) which can lead to a significant metabolic abnormality that is desirable or not desirable (Veneroni-Gouveia et al 2015;de Camargo et al 2015). IGFBP-3 gene expression was higher in the liver and longisimus dorsi muscles, thus contributing to the development of these organs (Zhang et al 2015), increased ADG from birth to weaning and weaning weight (Rasouli et al 2016), but there is not enough evidence to suggest that SNPs are associated with milk production (Zhou et al 2016).…”

Section: B I O D I V E R S I T a S 18 (2): 795-800 April 2017 798mentioning

confidence: 99%

Short Communication: Population structure of mangrove crab Scylla oceanica in mangrove ecosystem of Tanjung Lesung, Banten, Indonesia

et al. 2017

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Abstract. Priyadi DA, Panjono, Bintara S, Hartatik T. 2017. The genotype of gene sequences. Biodiversitas 18: 795-800. The IGFBP-3 gene is a potential marker of cattle growth, which is related to the functions that influence the growth, energy metabolism, reproduction and immunity. The genome was isolated from whole blood samples of 10 Brahman cattle and 16 Brahman Cross cattle. Cattle IGFBP-3 gene that targeted in this study was located in the part of intron 2, exon 3 and part of intron 3. The gene targets were amplified using specific primers by Polymerase Chain Reaction (PCR) technique, resulting 563 bp amplification product. As data comparison to reveal the SNP, the GenBank sequences (n = 14) from various breed and countries were used. The objective of this study was to reveal the SNP on the Brahman and Brahman Cross IGFBP-3 gene. The results obtained 3 genotypes from one SNP that spread in the sample population. The SNP was located in intron 2 at position 3,930 (GA). The Polymorphism could be recognized by PvuII restriction enzymes. There was not enough evidence to associate the SNP with the phenotype (at pre-weaning age) of Brahman and Brahman Cross cattle. There was a genetic diversity in the population studied. Knowledgeable SNP could be used as genetic markers for further research on Brahman and Brahman Cross cattle.

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“…IGFBPs, a family of six or more related proteins that bind IGF with high affinity, could sequester IGF to decrease protein synthesis and inhibit muscle cell differentiation. The affinity of IGFBP6 towards IGF2 surpasses that of other IGFBPs, and the expression level of IGFBP6 mRNA is highest in muscle tissue [41]. Additionally, it could enhance the muscle differentiation process by triggering predominantly the MAPK pathway in the absence of IGF2 [42].…”

Section: Discussionmentioning

confidence: 99%

Muscle Transcriptome Analysis of Mink at Different Growth Stages Using RNA-Seq

Rong,

Xing,

Zhang

2024

Biology

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Mink is a kind of small and precious fur animal resource. In this study, we employed transcriptomics technology to analyze the gene expression profile of mink pectoral muscle tissue, thereby elucidating the regulatory mechanisms underlying mink growth and development. Consequently, a total of 25,954 gene expression profiles were acquired throughout the growth and development stages of mink at 45, 90, and 120 days. Among these profiles, 2607 genes exhibited significant differential expression (|log2(fold change)| ≥ 2 and p_adj < 0.05). GO and KEGG enrichment analyses revealed that the differentially expressed genes were primarily associated with the mitotic cell cycle process, response to growth factors, muscle organ development, and insulin resistance. Furthermore, GSEA enrichment analysis demonstrated a significant enrichment of differentially expressed genes in the p53 signaling pathway at 45 days of age. Subsequent analysis revealed that genes associated with embryonic development (e.g., PEG10, IGF2, NRK), cell cycle regulation (e.g., CDK6, CDC6, CDC27, CCNA2), and the FGF family (e.g., FGF2, FGF6, FGFR2) were all found to be upregulated at 45 days of age in mink, which suggested a potential role for these genes in governing early growth and developmental processes. Conversely, genes associated with skeletal muscle development (PRVA, TNNI1, TNNI2, MYL3, MUSTN1), a negative regulator of the cell cycle gene (CDKN2C), and IGFBP6 were found to be up-regulated at 90 days of age, suggesting their potential involvement in the rapid growth of mink. In summary, our experimental data provide robust support for elucidating the regulatory mechanisms underlying the growth and development of mink.

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Molecular characterization and expression patterns of insulin-like growth factor-binding protein genes in postnatal Nanjiang brown goats

Cited by 6 publications

References 46 publications

Comparative Genomic Characterization of Insulin-Like Growth Factor Binding Proteins in Cattle and Buffalo

Comparative Genomic Characterization of Insulin-Like Growth Factor Binding Proteins in Cattle and Buffalo

Short Communication: Population structure of mangrove crab Scylla oceanica in mangrove ecosystem of Tanjung Lesung, Banten, Indonesia

Muscle Transcriptome Analysis of Mink at Different Growth Stages Using RNA-Seq

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