Molecular characterization and expression patterns of Spodoptera litura heat shock protein 70/90, and their response to zinc stress

Shu, Yinghua; Du, Yan-Jun; Wang, Jianwu

doi:10.1016/j.cbpa.2010.09.006

Cited by 72 publications

(37 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, unlike in several other insects (Colinet et al 2010;Wang and Kang 2005;Zhang and Denlinger 2010), GmHsp70 was not upregulated under cold stress. The similar expression patterns of GmHsp70 and GmHsp40 and rapid upregulation of the expression of these genes suggest a potential role of these co-chaperones in stress responses (Huang and Kang 2007;Kampinga and Craig 2010;Shu et al 2011;Xu et al 2011), but this needs further investigation such as through the use of RNAi to alter expression patterns. On the other hand, the absence of large changes in GmHsc70 expression under both heat and cold conditions, which has also been observed in other insects (Bettencourt et al 2008;Zhang and Denlinger 2010), suggests that this Hsp cognate is not thermally inducible and may not play a chaperone role under adverse conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Insect Hsp genes constitute a subset of a larger group of genes coding for molecular chaperones that are involved in multiple developmental processes and that assist repair stress injuries via transportation and degradation of aggregated proteins in the organism (Choi et al 2014;Kim et al 2014;Shu et al 2011). For example, Hsc70 and Hsp90 interact with ecdysone receptor activation which is vital in ecdysone response pathway (Arbeitman and Hogness 2000), while Hsp27 is known to involve in eye development (Chen et al 2012).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Response of heat shock protein genes of the oriental fruit moth under diapause and thermal stress reveals multiple patterns dependent on the nature of stress exposure

Zhang

Peng

Zheng

et al. 2016

Cell Stress and Chaperones

View full text Add to dashboard Cite

Heat shock protein gene (Hsp) families are thought to be important in thermal adaptation, but their expression patterns under various thermal stresses have still been poorly characterized outside of model systems. We have therefore characterized Hsp genes and their stress responses in the oriental fruit moth (OFM), Grapholita molesta, a widespread global orchard pest, and compared patterns of expression in this species to that of other insects. Genes from four Hsp families showed variable expression levels among tissues and developmental stages. Members of the Hsp40, 70, and 90 families were highly expressed under short exposures to heat and cold. Expression of Hsp40, 70, and Hsc70 family members increased in OFM undergoing diapause, while Hsp90 was downregulated. We found that there was strong sequence conservation of members of large Hsp families (Hsp40, Hsp60, Hsp70, Hsc70) across taxa, but this was not always matched by conservation of expression patterns. When the large Hsps as well as small Hsps from OFM were compared under acute and ramping heat stress, two groups of sHsps expression patterns were apparent, depending on whether expression increased or decreased immediately after stress exposure. These results highlight potential differences in conservation of function as opposed to sequence in this gene family and also point to Hsp genes potentially useful as bioindicators of diapause and thermal stress in OFM.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Response of heat shock protein genes of the oriental fruit moth under diapause and thermal stress reveals multiple patterns dependent on the nature of stress exposure

Zhang

Peng

Zheng

et al. 2016

Cell Stress and Chaperones

View full text Add to dashboard Cite

show abstract

“…HSP70s may not be the only proteins involved in heat and cold resistance; other proteins such as HSP20, HSP60, and HSP90 may also be involved in heat resistance (Zhang & Denlinger 2010;Wang et al 2012) or in the accumulation of low-molecular-weight cryoprotectants (Crowe et al 1988), synthesis of antifreeze proteins (Duman 2001), and remodeling of the structure of the cell membrane (Tomcala et al 2006). Some studies have found HSP70 to be up-regulated 2-5 fold in insects (Colinet et al 2010;Morales et al 2011;Shu et al 2011;Wang et al 2012;Yu et al 2012;Luo et al 2014). …”

Section: Discussionmentioning

confidence: 99%

Mamestra brassicae Nucleopolyhedrovirus Infection and Enhancing Effect of Proteins Derived From Xestia c-nigrum granulovirus in Larvae of Mamestra brassicae and Helicoverpa armigera (Lepidoptera: Noctuidae) on Cabbage

Mukawa

Goto

2010

View full text Add to dashboard Cite

“…The Hsp70 family, one of the most abundant HSP families, is highly conserved, is characterized by the highest transcript levels, and is the most sensitive to various harmful stimuli (Shu et al, 2011). All proteins of Hsp70 family comprise three distinct domains: an N-terminal adenosine triphosphatase (ATPase) domain (approximately 400 amino acids), a substrate binding domain (approximately 200 amino acids), and a highly variable C-terminal domain (Renner & Waters, 2007).…”

Section: Introductionmentioning

confidence: 99%

Identification of heat shock protein genes hsp70s and hsc70 and their associated mRNA expression under heat stress in insecticide-resistant and susceptible diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)

Zhang¹,

Wang²,

Jing³

et al. 2015

Eur. J. Entomol.

View full text Add to dashboard Cite

Abstract.To gain further insight into the molecular features of the ubiquitous Hsp70 family of conserved heat shock proteins, total nine full-length cDNA sequences of inducible hsp70s and one constitutive hsc70 (Px-hsc70(C)) were isolated and characterized in the diamondback moth (DBM), Plutella xylostella, collected from Fuzhou, China. The nine Px-hsp70s cDNAs encoded the protein of between 629-669 amino acids with molecular weight ranging from 69.00-72.58 kDa and were derived from four hsp70 genes in the genome of DBM. The Px-hsc70(C) cDNA contained 1,953 bp of open reading frame (ORF), which produced a putative protein comprising 650 amino acids with a calculated molecular weight of 71.18 kDa. Whether in adults or larvae of chlorpyrifos-resistant (R R ) and chlorpyrifos-susceptible (S S ) strains of DBM, the basal level (at 25°C) of Px-hsc70(C) mRNA expression was high, but no significant up-regulation expression was found under heat stress. However, heat stress facilitated up-regulation expressions of Px-hsp70s, and S S DBM displayed higher up-regulation expression of Px-hsp70s than R R DBM. We suggest that higher up-regulation expression of Px-hsp70s in S S DBM is probably involved in their higher thermal tolerance.

show abstract

Molecular characterization and expression patterns of Spodoptera litura heat shock protein 70/90, and their response to zinc stress

Cited by 72 publications

References 44 publications

Response of heat shock protein genes of the oriental fruit moth under diapause and thermal stress reveals multiple patterns dependent on the nature of stress exposure

Response of heat shock protein genes of the oriental fruit moth under diapause and thermal stress reveals multiple patterns dependent on the nature of stress exposure

<I>Mamestra brassicae</I> Nucleopolyhedrovirus Infection and Enhancing Effect of Proteins Derived From <I>Xestia c-nigrum granulovirus</I> in Larvae of <I>Mamestra brassicae</I> and <I>Helicoverpa armigera</I> (Lepidoptera: Noctuidae) on Cabbage

Identification of heat shock protein genes hsp70s and hsc70 and their associated mRNA expression under heat stress in insecticide-resistant and susceptible diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae)

Contact Info

Product

Resources

About