Molecular characterization and functional analyses of a hepcidin gene from Bostrychus sinensis

Shen, Bin; Wei, Ke; Yang, Jingjing; Jing, Fei; Zhang, Jianshe

doi:10.1016/j.aquaculture.2021.737114

Cited by 7 publications

(1 citation statement)

References 66 publications

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“…The transgenic TH2-3 (tilapia hepcidin2-3) zebrafish significantly up-regulated the expressions of Myd88, TLR1, and TLR3 to resist V. vulnificus infection [80]. Chinese black sleeper (Bostrychus sinensis) injected with a synthetic BsHep peptide increased the activation levels of immune-related genes (TLR1, TLR2, TLR5 and MyD88) in the TLR signaling pathway against V. parahemolyticus infection [81]. In this study, we found that the expressions of IL-6, TNF-α, IL-1β, IFN-γ, TLR1, and MyD88 were increased after the TroHepc2-22 injection, suggesting that the synthetic peptide TroHepc2-22 could regulate the expressions of immune-related genes and, hence, participate in the antibacterial immune response of the organism.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Activity and Mechanisms of TroHepc2-22, a Derived Peptide of Hepcidin2 from Golden Pompano (Trachinotus ovatus)

Zhang

Zhou

Zhang

et al. 2023

IJMS

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Hepcidin, a cysteine-rich antimicrobial peptide, has a highly conserved gene structure in teleosts, and it plays an essential role in host immune response against various pathogenic bacteria. Nonetheless, few studies on the antibacterial mechanism of hepcidin in golden pompano (Trachinotus ovatus) have been reported. In this study, we synthesized a derived peptide, TroHepc2-22, from the mature peptide of T. ovatus hepcidin2. Our results showed that TroHepc2-22 has superior antibacterial abilities against both Gram-negative (Vibrio harveyi and Edwardsiella piscicida) and Gram-positive (Staphylococcus aureus and Streptococcus agalactiae) bacteria. Based on the results of a bacterial membrane depolarization assay and propidium iodide (PI) staining assay in vitro, TroHepc2-22 displayed antimicrobial activity by inducing the bacterial membrane depolarization and changing the bacterial membrane permeability. Scanning electron microscopy (SEM) visualization illustrated that TroHepc2-22 brought about membrane rupturing and the leakage of the cytoplasm for the bacteria. In addition, TroHepc2-22 was verified to have hydrolytic activity on bacterial genomic DNA in view of the results of the gel retardation assay. In terms of the in vivo assay, the bacterial loads of V. harveyi in the tested immune tissues (liver, spleen, and head kidney) were significantly reduced in T. ovatus, revealing that TroHepc2-22 significantly enhanced the resistance against V. harveyi infection. Furthermore, the expressions of immune-related genes, including tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin 1-β (IL-1β), IL-6, Toll-like receptor 1 (TLR1), and myeloid differentiation factor 88 (MyD88) were significantly increased, indicating that TroHepc2-22 might regulate inflammatory cytokines and activate immune-related signaling pathways. To summarize, TroHepc2-22 possesses appreciable antimicrobial activity and plays a vital role in resisting bacterial infection. The observation of our present study unveils the excellent application prospect of hepcidin as a substitute for antibiotics to resist pathogenic microorganisms in teleosts.

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Section: Discussionmentioning

confidence: 99%