Molecular Characterization and Functional Analysis of a Ferritin Heavy Chain Subunit from the Eri-Silkworm, Samia cynthia ricini

Yu, Hai‐Zhong; Zhang, Shang-Zhi; Ma, Yan; Fei, Dong-Qiong; Li, Bing; Yang, Liang; Wang, Jie; Li, Zhen; Muhammad, Azharuddin; Xu, Jinghua

doi:10.3390/ijms18102126

Cited by 13 publications

(2 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar expression patterns can be observed in other insects, such as Bactrocera dorsalis , where both BdFer1HCH and BdFer2LCH are widely expressed in the midgut, fat body, ovaries, and integument (Jiang et al 2014). In Philosamia cynthia ricini , ScFerHCH was highly expressed in the fat body and midgut (Yu et al 2017b). Based on these results, we speculated that BtabFer1 may be involved in the female reproduction process.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies suggest that Fer is also involved in development regulation, cell activation, angiogenesis, and inflammation in chronic liver injury (KAMILARIS et al 1987, Ruddell et al 2009, Alkhateeb and Connor 2010). In swallowtail butterfly, Papilio xuthus , and the Eri-Silkworm, Samia cynthia ricini , Fer plays a key role in the defense of the body against bacterial infection (Chow et al 2010, Yu et al 2017a, Lu et al 2019). In the tick, Ixodes Ricinus, knockdown of the Fer1 and Fer2 genes has a negative effect on feeding abilities and hatching rate (Hajdusek et al 2009).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A ferritin protein is involved in the development and reproduction of the whitefly, Bemisia tabaci

Yu,

Wang,

Yang

et al. 2023

Environmental Entomology

View full text Add to dashboard Cite

Ferritins are conserved iron-binding proteins that exist in most living organisms and play an essential role in the maintenance of cellular iron homeostasis. Although ferritin has been studied in many species, little is known about its role in the whitefly, Bemisia tabaci. In this study, we identified an iron-binding protein from B. tabaci and named it BtabFer1. The full-length cDNA of BtabFer1 is 1,043 bp and encodes a protein consisting of 224 amino acids with a deduced molecular weight of 25.26 kDa, and phylogenetic analysis shows that BtabFer1 is conserved among Hemiptera insects. The expression levels of BtabFer1 in different developmental stages and tissues were analyzed by real-time PCR, and results showed that BtabFer1 was ubiquitously expressed at all developmental stages and in all examined tissues. The RNAi-mediated knockdown of BtabFer1 caused a significant reduction in survival rate, egg production, and egg hatching rate of whiteflies. Knockdown of BtabFer1 also inhibited the transcription of genes in the juvenile hormone signal transduction pathway. Taken together, these results suggest that BtabFer1 plays a critical role in the development and reproduction of whiteflies. This study can broaden our understanding of ferritin in insect fecundity and development, as well as provide baseline data for future studies.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A ferritin protein is involved in the development and reproduction of the whitefly, Bemisia tabaci

Yu,

Wang,

Yang

et al. 2023

Environmental Entomology

View full text Add to dashboard Cite

show abstract

Identification and functional analysis of an iron‐binding protein, ferritin heavy chain subunit, from the swallowtail butterfly, Papilio xuthus

Xie

et al. 2019

Arch Insect Biochem Physiol

Self Cite

View full text Add to dashboard Cite

Ferritin, which is ubiquitous among all living organisms, plays a crucial role in maintaining iron homeostasis, immune response, and detoxification. In the present research, we identified an iron-binding protein, ferritin heavy chain subunit, from Papilio xuthus and named PxFerHCH. The complete complementary DNA of PxFerHCH was 1,252 bp encoding a sequence of 211 amino acids, which includes an iron-responsive element. Phylogenetic analysis showed that PxFerHCH is clustered with Manduca sexta and Galleria mellonella ferritin heavy chain subunits. Expression levels of PxFerHCH in various tissues were analyzed by reverse transcription quantitative polymerase chain reaction, and the results exhibited that PxFerHCH was expressed in all tissues with the highest expression in the fat body. The relative expression level of PxFerHCH in response to bacterial (Escherichia coli and Staphylococcus aureus) challenges sharply increased by about 12 hr postinfection (hpi) and then decreased at 24 hpi. In addition, the iron-binding capacity and antioxidation activity of recombinant PxFerHCH protein were also investigated. These results reveal that PxFerHCH might play an important role in defense against bacterial infection.

show abstract

RNAi‐mediated silencing of ferritin genes in the brown planthopper Nilaparvata lugens affects survival, growth and female fecundity

Shen

Chen

Zhang

2020

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND: The brown planthopper (BPH), Nilaparvata lugens, is the most destructive rice insect pest. To exploit potential target genes for applications in transgenic rice to control this sap-sucking insect pest, three ferritin genes were functionally characterized in this study. RESULTS: In this study, three ferritin genes, that is, ferritin 1 Heavy Chain (NlFer1), ferritin 2 Light Chain (NlFer2) and soma ferritin (Nlsoma-Fer), were identified from BPH. Tissue-specific analyses showed that all three genes were highly expressed in the gut. Although double-stranded RNA injection-mediated RNA inference (RNAi) of Nlsoma-Fer expression resulted in only < 14% mortality in BPH, knockdown of NlFer1 or NlFer2 led to retarded growth and 100% mortality in young nymphs, and downregulation of NlFer1 and NlFer2 in newly emerged female adults caused undeveloped ovaries and severely inhibited oocyte growth, resulting in extremely low fecundity and a zero hatching rate. Knockdown of NlFer1 and NlFer2 caused similar phenotypes in BPH, indicating that they function together, as in many other animals. The results demonstrated that NlFer1 and NlFer2 were essential for BPH development and reproduction. BPHs showed high sensitivity to both dsNlFer1 and dsNlFer2, and injection of only 0.625 ng dsNlFer1 per BPH resulted in 100% mortality. Additionally, the effectiveness of feeding dsNlFer1 and dsNlFer2 to BPH nymphs was further proven. CONCLUSION: NlFer1 and NlFer2 are essential for BPH development and reproduction, and the insect is highly sensitive to their depletion, suggesting that the two gut-highly-expressed genes are promising candidates for application in RNAi-based control of this destructive pest.

show abstract

Molecular Characterization and Functional Analysis of a Ferritin Heavy Chain Subunit from the Eri-Silkworm, Samia cynthia ricini

Cited by 13 publications

References 63 publications

A ferritin protein is involved in the development and reproduction of the whitefly, Bemisia tabaci

A ferritin protein is involved in the development and reproduction of the whitefly, Bemisia tabaci

Identification and functional analysis of an iron‐binding protein, ferritin heavy chain subunit, from the swallowtail butterfly, Papilio xuthus

RNAi‐mediated silencing of ferritin genes in the brown planthopper Nilaparvata lugens affects survival, growth and female fecundity

Contact Info

Product

Resources

About