Molecular characterization and functional analysis of Eimeria tenella malate dehydrogenase

Chen, Ting; Huang, Bing; Zhao, Qiping; Dong, Hui; Zhu, Shanfeng; Zhao, Zongping; Lv, Ling; Yan, Ming

doi:10.1007/s00436-018-5875-x

Cited by 8 publications

(4 citation statements)

References 40 publications

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“…2019) using rabbit anti‐ E. tenella sporozoite serum (Chen et al. 2018) (dilutions 1:100) or mouse monoclonal anti‐GST tag antibody (Beyotime, 1:2,000 dilution) as the primary antibodies. Determinations of the purified protein concentrations were performed using the BCA protein assay kit (Beyotime, Haimen, China).…”

Section: Methodsmentioning

confidence: 99%

Identification and Characterization of a Novel Apical Membrane Antigen 3 in Eimeria tenella

Wang

Zhu

Zhao

et al. 2021

J Eukaryotic Microbiology

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Eimeria tenella is an obligate intracellular parasite in the phylum Apicomplexa. As described for other members of Apicomplexa, apical membrane antigen 1 (AMA1) has been shown to be critical for sporozoite invasion of host cells by E. tenella. Recently, an E. tenella paralogue of AMA1 (EtAMA1), dubbed sporoAMA1 (EtAMA3), was identified in proteomic and transcriptomic analyses of E. tenella, but not further characterized. Here, we show that EtAMA3 is a type I integral membrane protein that has 24% −38% identity with other EtAMAs. EtAMA3 has the same pattern of Cys residues in domains I and II of AMA1 orthologs from apicomplexan parasites, but high variance in domain III, with all six invariant Cys residues absent. EtAMA3 expression was developmentally regulated at the mRNA and protein levels. EtAMA3 protein was detected in sporulated oocysts and sporozoites, but not in the unsporulated oocysts or second‐generation merozoites. EtAMA3 is secreted by micronemes and is primarily localized to the apical end of sporozoites during host‐cell invasion. Additionally, pretreatment of sporozoites with rEtAMA3‐specific antibodies substantially impeded their invasion into host cells. These results suggest EtAMA3 is a sporozoite‐specific protein that is involved in host‐cell sporozoite invasion.

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Section: Methodsmentioning

confidence: 99%

Identification and Characterization of a Novel Apical Membrane Antigen 3 in Eimeria tenella

Wang

Zhu

Zhao

et al. 2021

J Eukaryotic Microbiology

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“…Chemical synthetic drugs include a wide range, such as sulfachloropyrazine sodium, amproline, toltrazuril, clopidol, and nicarbazine ( Greif et al . , 2001 ; Chen et al . , 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Pathogenicity and drug resistance of the Eimeria tenella isolate from Yiwu, Zhejiang province, eastern China

Hao

et al. 2023

Poultry Science

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“…Based on our preliminary results, we speculated that the ChTLR15/ChNF-κB-ChNLRP3/ChIL-1β signaling pathway may be specifically activated by Eimeria sporozoites, which mediate inflammatory injury during Eimeria infection. It was reported that chicken embryo fibroblast cells (DF-1) can be directly invaded by Eimeria sporozoites and have been extensively applied to study innate immune responses [15][16][17]. To verify the above hypothesis, the ChTLR15/ChNF-κB and ChNLRP3/ChIL-1β signaling pathways were analyzed in DF1 cells at the transcriptional and protein levels using small interfering RNA (siRNA) and overexpression technology.…”

Section: Introductionmentioning

confidence: 99%

Activation of ChTLR15/ChNF-κB-ChNLRP3/ChIL-1β signaling transduction pathway mediated inflammatory responses to E. tenella infection

Yang

Jia

et al. 2021

Vet Res

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Avian coccidiosis caused by Eimeria leads to severe economic losses in the global poultry industry. Although chicken Toll-like receptor 15 (ChTLR15) was reported to be involved in Eimeria infection, the detailed mechanism underlying its role in the inflammatory response remains to be discovered. The present study demonstrated that the mRNA expression levels of ChTLR15, ChMyD88, ChNF-κB, ChNLRP3, ChCaspase-1, ChIL-18 and ChIL-1β and the protein levels of ChTLR15 and ChNLRP3 in cecal tissues of Eimeria-infected chickens were significantly elevated at 4, 12, and 24 h compared with those in noninfected control chickens (p < 0.01). Moreover, the mRNA levels of molecules in the ChTLR15/ChNF-κB and ChNLRP3/ChIL-1β pathways and the protein levels of ChTLR15 and ChNLRP3 in chicken embryo fibroblast cells (DF-1) stimulated by E. tenella sporozoites were consistent with those in Eimeria-infected chickens. Furthermore, overexpression of ChTLR15 in DF1 cells augmented activation of the ChTLR15/ChNF-κB and ChNLRP3/ChIL-1β pathways when stimulated with E. tenella sporozoites, while knockdown of ChTLR15 in DF1 cells showed inverse effects. Taken together, the present study provides evidence that E. tenella sporozoites specifically activate ChTLR15 and then trigger activation of the ChNLRP3/ChIL-1β pathway, which partially mediates inflammatory responses to Eimeria infection.

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Molecular characterization and functional analysis of Eimeria tenella malate dehydrogenase

Cited by 8 publications

References 40 publications

Identification and Characterization of a Novel Apical Membrane Antigen 3 in Eimeria tenella

Identification and Characterization of a Novel Apical Membrane Antigen 3 in Eimeria tenella

Pathogenicity and drug resistance of the Eimeria tenella isolate from Yiwu, Zhejiang province, eastern China

Activation of ChTLR15/ChNF-κB-ChNLRP3/ChIL-1β signaling transduction pathway mediated inflammatory responses to E. tenella infection

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