Molecular characterization and methylation study of matrix gla protein in articular cartilage from pig with osteochondrosis

Laenoi, Watchara; Uddin, Muhammad Jasim; Çınar, Mehmet Ulaş; Phatsara, C.; Tesfaye, Dawit; Scholz, Armin M.; Tholen, Ernst; Looft, Christian; Mielenz, M.; Sauerwein, H.; Schellander, K.

doi:10.1016/j.gene.2010.03.009

Cited by 20 publications

(11 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The histological examination assessed cartilage thickness, cartilage degradation and the vessel structure of cartilage canals. The histological procedures that were used have been described by Laenoi et al [24]. The number of animals with OC on different joints ranged between 274 and 279 (Table 1).…”

Section: Methodsmentioning

confidence: 99%

Quantitative trait loci analysis for leg weakness-related traits in a Duroc × Pietrain crossbred population

et al. 2011

Self Cite

View full text Add to dashboard Cite

BackgroundLeg weakness issues are a great concern for the pig breeding industry, especially with regard to animal welfare. Traits associated with leg weakness are partly influenced by the genetic background of the animals but the genetic basis of these traits is not yet fully understood. The aim of this study was to identify quantitative trait loci (QTL) affecting leg weakness in pigs.MethodsThree hundred and ten F2 pigs from a Duroc × Pietrain resource population were genotyped using 82 genetic markers. Front and rear legs and feet scores were based on the standard scoring system. Osteochondrosis lesions were examined histologically at the head and the condylus medialis of the left femur and humerus. Bone mineral density, bone mineral content and bone mineral area were measured in the whole ulna and radius bones using dual energy X-ray absorptiometry. A line-cross model was applied to determine QTL regions associated with leg weakness using the QTL Express software.ResultsEleven QTL affecting leg weakness were identified on eight autosomes. All QTL reached the 5% chromosome-wide significance level. Three QTL were associated with osteochondrosis on the humerus end, two with the fore feet score and two with the rear leg score. QTL on SSC2 and SSC3 influencing bone mineral content and bone mineral density, respectively, reached the 5% genome-wide significance level.ConclusionsOur results confirm previous studies and provide information on new QTL associated with leg weakness in pigs. These results contribute towards a better understanding of the genetic background of leg weakness in pigs.

show abstract

Section: Methodsmentioning

confidence: 99%

Quantitative trait loci analysis for leg weakness-related traits in a Duroc × Pietrain crossbred population

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…Table S1). Cartilage canal necrosis was included as a diagnostic feature in only 2 sets of criteria, 7,57 referred to in 3 of the studies listed in Supplemental Table S1. Thus, it would be possible to discuss the relationship between other studies and osteochondrosis manifesta or OCD, but this would reveal limited information about pathogenesis and would be more appropriate in a clinically oriented paper than in the current review.…”

Section: What Is the Relationship Between The Studies Of Ischemic Chomentioning

confidence: 99%

“…Discussion of the relationship between other studies and osteochondrosis latens would be confined to the 2 cases where cartilage canal necrosis was included as a diagnostic feature. 7,57 The 3 studies in question focus on genetics, and genetic studies were the most common among studies into aspects of osteochondrosis other than pathogenesis, representing 8 of 22 of the studies in pigs (36%) and 26 of 92 of the studies in horses (28%; Suppl. Table S1).…”

Section: What Is the Relationship Between The Studies Of Ischemic Chomentioning

confidence: 99%

An Update on the Pathogenesis of Osteochondrosis

2015

View full text Add to dashboard Cite

Osteochondrosis is defined as a focal disturbance in endochondral ossification. The cartilage superficial to an osteochondrosis lesion can fracture, giving rise to fragments in joints known as osteochondrosis dissecans (OCD). In pigs and horses, it has been confirmed that the disturbance in ossification is the result of failure of the blood supply to epiphyseal growth cartilage and associated ischemic chondronecrosis. The earliest lesion following vascular failure is an area of ischemic chondronecrosis at an intermediate depth of the growth cartilage (osteochondrosis latens) that is detectable ex vivo, indirectly using contrast-enhanced micro-and conventional computed tomography (CT) or directly using adiabatic T1r magnetic resonance imaging. More chronic lesions of ischemic chondronecrosis within the ossification front (osteochondrosis manifesta) are detectable by the same techniques and have also been followed longitudinally in pigs using plain CT. The results confirm that lesions sometimes undergo spontaneous resolution, and in combination, CT and histology observations indicate that this occurs by filling of radiolucent defects with bone from separate centers of endochondral ossification that form superficial to lesions and by phagocytosis and intramembranous ossification of granulation tissue that forms deep to lesions. Research is currently aimed at discovering the cause of the vascular failure in osteochondrosis, and studies of spontaneous lesions suggest that failure is associated with the process of incorporating blood vessels into the advancing ossification front during growth. Experimental studies also show that bacteremia can lead to vascular occlusion. Future challenges are to differentiate between causes of vascular failure and to discover the nature of the heritable predisposition for osteochondrosis.

show abstract

“…For lesion scoring, histological sections of 7 μm were prepared and stained in duplicate after fixation and demineralization. Representative section were scored on a scale from 1 (=normal) to 4 (=severe) (score 1 denotes homogenous cartilage matrix; columns of chondrocytes visible, score 2 means homogenous cartilage matrix; low-grade fraying of the surface, score 3 shows surface erosions, fraying in deeper cartilage layers, score 4 means surface erosions, fraying in deeper cartilage layers and cavitations) (Laenoi et al, 2010; Rangkasenee et al, 2013). Finally, four divergent sib pairs with low (1) and high (4) histologic OC lesion scores were selected for RNA preparation and subsequent real-time RT-PCR.…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Association Identifies TBX5 as Candidate Gene for Osteochondrosis Providing a Functional Link to Cartilage Perfusion as Initial Factor

Rangkasenee¹,

Muráni²,

Brunner³

et al. 2013

Front. Genet.

Self Cite

View full text Add to dashboard Cite

Osteochondrosis (OC) is an orthopedic syndrome of the joints that occurs in children and adolescents and domestic animals, particularly pigs, horses, and dogs. OC is the most frequent cause of leg weakness in rapidly growing pigs causing animal welfare issues and economic losses. In this study, a genome-wide association study (GWAS) was performed using the Porcine 60k SNPChip in animals of the breed Large White (n = 298) to identify chromosome regions and candidate genes associated with OC lesion scores. A total of 19 SNPs on chromosomes (SSC) 3, 5, 8, 10, 14, and 18 were significantly associated with OC lesion scores (p-values ≤ 10−5). The SNPs MARC0098684, MARC00840086, MARC0093124, and ASGA0062794 at SSC14 36.1–38.2 Mb encompass a region of six linkage disequilibrium (LD) blocks. The most significant SNP ASGA0062794 is located in a LD block spanning 465 kb and covering the gene encoding T-box transcription factor 5 (TBX5). A SNP (c.54T > C) identified in TBX5 was significantly associated with OC lesion scores in a single-marker analysis. TBX5 c.54T > C showed highest LD with ASGA00627974 (r2 = 0.96) and superior association with OC lesion scores over other SNPs when included in the genome scan, whereas its treatment as an additional fixed effect in the GWAS statistical model led to a drop of significance of nearby markers. Moreover, real-time PCR showed different transcript abundance of TBX5 in healthy and defect cartilage. The results imply that the association signal obtained on SCC14 is largely attributable to TBX5 c.54T > C likely to be in LD with a regulatory polymorphism of TBX5. The transcription factor TBX5 interacts with GJA5 and MEF2C both being involved in vascularization. This study provides evidence for epistatic interaction of TBX5 and MEF2C, thus supporting deficiency of blood supply to growth cartilage as being fundamental for the initiation of OC.

show abstract

Molecular characterization and methylation study of matrix gla protein in articular cartilage from pig with osteochondrosis

Cited by 20 publications

References 36 publications

Quantitative trait loci analysis for leg weakness-related traits in a Duroc × Pietrain crossbred population

Quantitative trait loci analysis for leg weakness-related traits in a Duroc × Pietrain crossbred population

An Update on the Pathogenesis of Osteochondrosis

Genome-Wide Association Identifies TBX5 as Candidate Gene for Osteochondrosis Providing a Functional Link to Cartilage Perfusion as Initial Factor

Contact Info

Product

Resources

About