Molecular characterization and multilocus genotypes of Enterocytozoon bieneusi among horses in southwestern China

Deng, Lei; Li, Wei; Zhong, Zhijun; Gong, Chao; Liu, Xuehan; Xiao-hong, Huang; Xiao, Li; Zhao, Ruoxuan; Wang, Wuyou; Feng, Fan; Zhang, Yue; Hu, Yanchun; Fu, Hualin; He, Min; Wu, Kui; Peng, Guangneng

doi:10.1186/s13071-016-1844-3

Cited by 42 publications

(34 citation statements)

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“…In Peru, India and Nigeria, 66 MLGs were identified in HIV-infected individuals [33,37]. In China, 59 and 44 MLGs were identified in NHPs and pigs, respectively, while some genetic diversity based on MLST data was also observed in E. bieneusi isolates from domestic, wild, and zoo animals [29,36,[42][43][44][45][46][47][48]. However, few studies analyzed the MLGs of this pathogen in NHPs in China.…”

Section: Discussionmentioning

confidence: 99%

Multilocus sequence typing of Enterocytozoon bieneusi in crab-eating macaques (Macaca fascicularis) in Hainan, China

Chen

Guo

et al. 2020

Parasites Vectors

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Background: Enterocytozoon bieneusi is one of common intestinal pathogens in humans and animals including nonhuman primates (NHPs). Many zoonotic pathogens including E. bieneusi have been found in these animals. However, there are few studies on the population structure of E. bieneusi in NHPs. To infer the gene diversity and population genetics of E. bieneusi, we selected 88 E. bieneusi-positive samples from crab-eating macaques for multilocus characterizations in this study.Methods: The E. bieneusi isolates examined belonged to three common genotypes with different host ranges by sequence analysis of the ribosomal internal transcribed spacer (ITS): Type IV (n = 44), Macaque3 (n = 24) and Peru8 (n = 20). They were further characterized by sequence analysis at four microsatellite and minisatellite loci (MS1, MS3, MS4 and MS7). DnaSP, Arlequin and LIAN were used to analyze the sequence data together with those from the ITS locus to infer the population genetics. Subpopulation structure was inferred using phylogenetic and STRU CTU RE analyses.Results: Seventy-two (81.8%), 71 (80.7%), 76 (86.4%) and 79 (89.8%) samples were amplified and sequenced successfully at the MS1, MS3, MS4 and MS7 loci, respectively, with 53 having sequence data at all five MLST loci including ITS. Altogether, 33 multilocus genotypes (MLGs) were produced based on concatenated sequences from the 53 samples. In phylogenetic analyses of sequences and allelic data, four major subpopulations (SPs) were observed with different ITS genotypes in each of them: Type IV and Peru8 in SP1 and SP2; Type IV, Macaque3 and Peru8 in SP3; and Type IV and Macaque3 in SP4. SP3 and SP4 were phylogenetically related and might be NHP-specific based on the fact that Macaque3 is mostly found in NHPs. A strong linkage disequilibrium (LD) was observed among the multilocus sequences and allelic data. Conclusions:The significant LD in the multilocus sequence analysis indicated the presence of an overall clonal population structure of E. bieneusi in crab-eating macaques. The inconsistent segregation of MLGs among ITS genotypes © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article' s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article' Publisher's NoteSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Section: Discussionmentioning

confidence: 99%

Multilocus sequence typing of Enterocytozoon bieneusi in crab-eating macaques (Macaca fascicularis) in Hainan, China

Chen

Guo

et al. 2020

Parasites Vectors

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“…ITS gen bölgesi sekanslarının filogenetik analizlerine göre E. bieneusi genotiplerinin 9 farklı genogruba bölündüğü ortaya konmuştur (10). Genogrup 1 en geniş grup olup günümüze kadar rapor edilen E. bieneusi genotiplerinin %94'ünü ihtiva etmekte ve buradaki genotiplerin insanlarda da enfeksiyon oluşturmalarından dolayı zoonotik grup olarak nitelendirilmektedir (11,12). Buna karşın diğer sekiz major genogrupta (grup 2-9) yer alan genotiplerin ise çoğunlukla spesifik konaklarda ve atık sularda bulunduğu kaydedilmiştir (11,13,14).…”

Section: Introductionunclassified

“…Genogrup 1 en geniş grup olup günümüze kadar rapor edilen E. bieneusi genotiplerinin %94'ünü ihtiva etmekte ve buradaki genotiplerin insanlarda da enfeksiyon oluşturmalarından dolayı zoonotik grup olarak nitelendirilmektedir (11,12). Buna karşın diğer sekiz major genogrupta (grup 2-9) yer alan genotiplerin ise çoğunlukla spesifik konaklarda ve atık sularda bulunduğu kaydedilmiştir (11,13,14). Bugüne kadar sığırlarda çoğunluğu genogrup 2'de olmak üzere 40'ın üzerinde E. bieneusi genotipi karakterize edilmiştir (15)(16)(17).…”

Section: Introductionunclassified

Molecular Prevalence and Phylogenetic Characterization of <i>Enterocytozoon bieneusi</i> in Healthy Cattle

Bilgin

Usluğ

Karademir

et al. 2020

Turkiye Parazitol Derg

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In this study, it was aimed to determine the molecular prevalence and genotypes of Enterocytozoon in healthy cattle. Methods: Fecal samples were collected from 50 cattle in Sivas between October 2017 and March 2018 and genomic DNA (gDNA) isolations were performed. gDNA isolates were processed by Nested PCR specifically amplifying ITS rRNA gene region to identify E. bieneusi. ITS rRNA region of E. bieneusi positive isolates were sequenced for genotyping and phylogenetic analyzes. Obtained sequences were assembled with appropriative genetic software, then phylogenetic relationships were revealed. Results: According to Nested PCR analyses, 29 (19.3%) out of totally examined samples were found positive for E. bieneusi. As a result of the sequence analyses, five distinct genotypes were determined. The most frequent genotype ERUSS1 and the other ERUSS2-4 genotypes were characterized as close to each other, which was reported for the first time in the world. Two isolates were determined in N genotype that was reported from cattle in Germany and were more different from the other genotypes. Phylogenetic analysis revealed that all the genotypes characterized in the study belonged to the genogroup 2. Conclusion: First molecular epidemiological data on E. bieneusi in cattle from Turkey were obtained with this study.

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“…PCR-based molecular techniques may be used to analyze the E. bieneusi genome, and for diagnosis. Based on the nested PCR amplification of internal transcribed spacers (ITS) of small subunits of ribosomal rRNA (SSU rRNA), over 240 E. bieneusi genotypes have been identified globally[9–11]. Phylogenetic analysis reveals that these genotypes clustered into nine groups.…”

Section: Introductionmentioning

confidence: 99%

“…Phylogenetic analysis reveals that these genotypes clustered into nine groups. Group 1 is considered zoonotic, and is composed of genotypes from humans and a few animals, while groups 2–9 have particular host associations or are found in wastewater[5, 11]. To better comprehend E. bieneusi genetic diversity and molecular characteristics, high-resolution multi-locus sequence typing (MLST) using three microsatellites (MS1, MS3 and MS7) and one minisatellite (MS4) as markers was used to explore genotype taxonomy and transmission routes [9, 12, 13].…”

Section: Introductionmentioning

confidence: 99%

First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China

Luo

Xiang

Liu

et al. 2018

Preprint

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24Enterocytozoon bieneusi is a common intestinal pathogen and a major cause of 25 diarrhea and enteric diseases in a variety of animals. While the E. bieneusi genotype 26 has become better-known, there are few reports on its prevalence in the Tibetan pig. 27 This study investigated the prevalence, genetic diversity, and zoonotic potential of E. 28 bieneusi in the Tibetan pig in southwestern China. Tibetan pig feces (266 samples) 29 were collected from three sites in the southwest of China. Feces were subjected to 30 PCR amplification of the internal transcribed spacer (ITS) region. E. bieneusi was 31 detected in 83 (31.2%) of Tibetan pigs from the three different sites, with 25.4% in 32 Kangding, 56% in Yaan and 26.7% in Qionglai. Age group demonstrated the 33 prevalence of E. bieneusi range from 24.4%( aged 0 to 1 years) to 44.4%( aged 1 to 2 34 years). Four genotypes of E. bieneusi were identified: two known genotypes EbpC 35 (n=58), Henan-IV (n=24) and two novel genotypes, SCT01 and SCT02 (one of each). 36Phylogenetic analysis showed these four genotypes clustered to group 1 with zoonotic 37 potential. Multilocus sequence typing (MLST) analysis three microsatellites (MS1, 38 MS3, MS7) and one minisatellite (MS4) revealed 47, 48, 23 and 47 positive 39 specimens were successfully sequenced, and identified ten, ten, five and five 40 genotypes at four loci, respectively. This study indicates the potential danger of E. 41 bieneusi to Tibetan pigs in southwestern China, and offers basic data for preventing 42 and controlling infections.43 44 45 Microsporidia are obligate intracellular eukaryotic pathogens, classified as fungi, 46 which are composed of approximately 1300 species in 160 genera[1]. To date, 17 47 microsporidia species are known to infect humans, and of these, E. bieneusi is the 48 most prevalent, accounting for over 90% of cases of human microsporidiosis[2]. 49 Since its first detection in an HIV/AIDS patient in 1985, a growing literature attests to 50 E. bieneusi expanding range of hosts [3-5]. In humans, infection by microsporidia 51 results in self-limiting diarrhea and malabsorption, most seriously, 52 immunocompromised and immunocompetent patients more susceptible to E. bieneusi 53 infection[6]. Normally, fecal-oral routes serve as the main infection pathways in 54 humans and animals, while human inhalation of E. bieneusi spores has also been 55 documented[7, 8]. 56 PCR-based molecular techniques may be used to analyze the E. bieneusi genome, 57 and for diagnosis. Based on the nested PCR amplification of internal transcribed 58 spacers (ITS) of small subunits of ribosomal rRNA (SSU rRNA), over 240 E. 59bieneusi genotypes have been identified globally [9][10][11]. Phylogenetic analysis reveals 60 that these genotypes clustered into nine groups. Group 1 is considered zoonotic, and is 61 composed of genotypes from humans and a few animals, while groups 2-9 have 62 particular host associations or are found in wastewater [5, 11]. To better comprehend 63 E. bieneusi genetic diversity and molecu...

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Molecular characterization and multilocus genotypes of Enterocytozoon bieneusi among horses in southwestern China

Cited by 42 publications

References 28 publications

Multilocus sequence typing of Enterocytozoon bieneusi in crab-eating macaques (Macaca fascicularis) in Hainan, China

Multilocus sequence typing of Enterocytozoon bieneusi in crab-eating macaques (Macaca fascicularis) in Hainan, China

Molecular Prevalence and Phylogenetic Characterization of <i>Enterocytozoon bieneusi</i> in Healthy Cattle

First report and multilocus genotyping of Enterocytozoon bieneusi from Tibetan pigs in southwestern China

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