Molecular Characterization and Phylogenetic Analyses of Heavy Metal Removal Bacteria from the Persian Gulf

Zolgharnein, Hossein; Karami, Kazem; Assadi, Mahnaz Mazaheri; Sohrab, Ali Dadolahi

doi:10.3923/biotech.2010.1.8

Cited by 16 publications

(7 citation statements)

References 26 publications

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“…This result was also found to be consistent with the observation of Rahman et al in case of formation of long-chain in response to arsenic stress compared to untreated cells indicating a probable tactic for accumulation of As inside their cells as well as contributing in As bioremediation 19 . Due to the combined uptake of Cd and Pb and to adapt to toxic conditions, the cell size of Pseudomonas aeruginosa was increased as observed by Zolgharnein 59 which had also a similarity with our SEM imaging results. The intracellular As bioaccumulation ability make our strain useful for biological detoxification of arsenic.…”

Section: Discussionsupporting

confidence: 89%

Bioaccumulation and detoxification of trivalent arsenic by Achromobacter xylosoxidans BHW-15 and electrochemical detection of its transformation efficiency

Diba

Khan²,

Uddin

et al. 2021

Sci Rep

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Arsenotrophic bacteria play an essential role in lowering arsenic contamination by converting toxic arsenite [As (III)] to less toxic and less bio-accumulative arsenate [As (V)]. The current study focused on the qualitative and electrocatalytic detection of the arsenite oxidation potential of an arsenite-oxidizing bacteria A. xylosoxidans BHW-15 (retrieved from As-contaminated tube well water), which could significantly contribute to arsenic detoxification, accumulation, and immobilization while also providing a scientific foundation for future electrochemical sensor development. The minimum inhibitory concentration (MIC) value for the bacteria was 15 mM As (III). Scanning Electron Microscopy (SEM) investigation validated its intracellular As uptake capacity and demonstrated a substantial association with the MIC value. During the stationary phase, the strain’s As (III) transformation efficiency was 0.0224 mM/h. Molecular analysis by real-time qPCR showed arsenite oxidase (aioA) gene expression increased 1.6-fold in the presence of As (III) compared to the untreated cells. The immobilized whole-cell also showed As (III) conversion up to 18 days. To analyze the electrochemical oxidation in water, we developed a modified GCE/P-Arg/ErGO-AuNPs electrode, which successfully sensed and quantified conversion of As (III) into As (V) by accepting electrons; implying a functional As oxidase enzyme activity in the cells. To the best of our knowledge, this is the first report on the electrochemical observation of the As-transformation mechanism with Achromobactersp. Furthermore, the current work highlighted that our isolate might be employed as a promising candidate for arsenic bioremediation, and information acquired from this study may be helpful to open a new window for the development of a cost-effective, eco-friendly biosensor for arsenic species detection in the future.

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Section: Discussionsupporting

confidence: 89%

Bioaccumulation and detoxification of trivalent arsenic by Achromobacter xylosoxidans BHW-15 and electrochemical detection of its transformation efficiency

Diba

Khan²,

Uddin

et al. 2021

Sci Rep

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“…However, microorganisms exposed to the higher concentration of toxic heavy metals have evolved various methods to resist metal stress [5]. One of the microbial processes as a bioremediation tool to remove heavy metals from soils is bioaccumulation [6], which is an active method dependent upon the metabolic energy of microorganisms [7]. Metals can also cause severe toxicity to the metabolic activities of various legumes including photosynthesis, synthesis of proteins, of enzymes, and of carbohydrates.…”

Section: Introductionmentioning

confidence: 99%

Impact of dual inoculation with Rhizobium and PGPR on growth and antioxidant status of Vicia faba L. under copper stress

Fatnassi

Chiboub

Saadani

et al. 2015

Comptes Rendus. Biologies

106

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Plant-growth-promoting bacteria (PGPR) may help reduce the toxicity of heavy metals in plants in polluted environments. In this work, the effects of dual inoculation with Rhizobium and PGPR strains on the growth of Vicia faba grown under copper stress were assessed during hydroponic culture. Growth parameters, copper (Cu) accumulation and antioxidant enzyme activities were studied. Copper concentrations above 1mM damaged plant growth, but co-inoculation reduced its harmful effect. Co-inoculation of plants treated with 1mM Cu and 2mM Cu increased the dry weights as compared with Cu-treated and uninoculated plants. However, it decreased copper uptake up to 80% in the roots of 1-mM-Cu-treated plants as compared to non-inoculated control. Copper tolerance in Vicia faba is linked to the activity of antioxidant systems that are modulated by metal concentrations: both superoxide dismutase (SOD) and catalase (CAT) were higher in the presence of Cu; a lower Cu dose of 0.5mM stimulated ascorbate peroxidase (APX) and/or peroxidase (POX) activities in shoots and roots; however in nodules CAT appeared to be the main peroxidase in H2O2 scavenging. The 1mM Cu treatment enhanced SOD, CAT and APX activities in roots and only SOD and POX were activated in shoots. All enzyme activities were inhibited by inoculation of 2mM Cu. The effect of inoculation with copper-accumulating PGPRs and the status of the antioxidant enzyme system were linked to changes in the copper tolerance status of Vicia faba. Our results suggested that Vicia faba inoculation with Rhizobium and PGPR Enterobacter clocae and Pseudomonas sp. could help to alleviate copper stress under hydroponic conditions. This result should be tested under field conditions for soil fertilization and phytostabilisation purposes.

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“…Therefore, there is a need for an accurate method for the monitoring and selection of metal resistant microorganisms to be used for biosorption. In the bioremediation literature, the selection of microorganisms for biosorption by culture-dependent and culture-independent (PCR-based molecular methods) approaches has been presented. − Conventional culture methods are based on isolation, classification, and characterization of microbial species . Unpredictability of growth timing, underestimation of bacterial colonies, and other time-consuming procedures lead to difficulties in the screening and differentiation of full microbial populations. − Culture-independent techniques such as extraction of nucleic acids, polymerase chain reaction (PCR) amplification, and DNA cloning and sequencing have eliminated the error originating from culture-dependent techniques .…”

mentioning

confidence: 99%

Quick Discrimination of Heavy Metal Resistant Bacterial Populations Using Infrared Spectroscopy Coupled with Chemometrics

et al. 2015

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Lead and cadmium are frequently encountered heavy metals in industrially polluted areas. Many heavy metal resistant bacterial strains have a high biosorption capacity and thus are good candidates for the removal of toxic metals from the environment. However, as of yet there is no accurate method for discrimination of highly adaptive bacterial strains among the populations present in a given habitat. In this study, we aimed to find distinguishing molecular features of lead and cadmium resistant bacteria using Attenuated Total Reflectance-Fourier Transformed Infrared (ATR-FT-IR) spectroscopy and chemometric approaches. Our results demonstrated that both control and metal exposed E. coli and S. aureus strains could be successfully discriminated from each other using hierarchical cluster and principal component analysis methods. Moreover, we found that lead exposed bacterial strains could be successfully discriminated from cadmium exposed ones with a high heterogeneity value. These clear discriminations can be described by the ability of a bacterium to change its metabolism in terms of the content and structure of cellular macromolecules under heavy metal stress. In our case, cadmium and lead-induced genetic response systems in bacteria caused remarkable alterations in overall cellular metabolism. Bacteria deal with a heavy metal stress by altering nucleic acid methylations and lipid and protein synthesis. Heavy metal burden led to the development of relevant metabolic changes in proteins, lipids, and nucleic acids of the resistant bacteria described in this study. Our approach showed that infrared spectra obtained via ATR-FT-IR spectroscopy coupled with chemometric analysis can be utilized for rapid, low-cost, informative, reliable, and operator-independent discrimination of resistant bacterial populations.

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Molecular Characterization and Phylogenetic Analyses of Heavy Metal Removal Bacteria from the Persian Gulf

Cited by 16 publications

References 26 publications

Bioaccumulation and detoxification of trivalent arsenic by Achromobacter xylosoxidans BHW-15 and electrochemical detection of its transformation efficiency

Bioaccumulation and detoxification of trivalent arsenic by Achromobacter xylosoxidans BHW-15 and electrochemical detection of its transformation efficiency

Impact of dual inoculation with Rhizobium and PGPR on growth and antioxidant status of Vicia faba L. under copper stress

Quick Discrimination of Heavy Metal Resistant Bacterial Populations Using Infrared Spectroscopy Coupled with Chemometrics

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