Molecular Characterization and Phylogeny of Chicken Anemia Virus Detected in Broiler Poultry Flocks in Punjab, Pakistan

Nadeem, Syed Maaz; Khan, Muti Ur Rehman; Aslam, Asim; Sheikh, Ali Ahmad; Ahmad, Arfan; Anees, Muhammad

doi:10.17582/journal.pjz/2020.52.1.sc14

Cited by 1 publication

(1 citation statement)

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“…Advances in pathogens biology and recombinant DNA technology are setting foundations in providing novel approaches to overcome the problems encountered with conventional vaccines design (Khan et al, 2016;Nadeem et al, 2020). Vaccine production has continued to be influenced by conventional vaccine preparation methods such as the use of existing wellcharacterized vectors, expression of discrete viral proteins and/or component(s), and production of virus-like particles (VLPs).…”

Section: Introductionmentioning

confidence: 99%

Application of CRISPR/Cas9 in Understanding Avian Viruses and Developing Poultry Vaccines

Vilela

Rohaim

Munir

2020

Front. Cell. Infect. Microbiol.

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Clustered regularly interspaced short palindromic repeats associated protein nuclease 9 (CRISPR-Cas9) technology offers novel approaches to precisely, cost-effectively, and user-friendly edit genomes for a wide array of applications and across multiple disciplines. This methodology can be leveraged to underpin host-virus interactions, elucidate viral gene functions, and to develop recombinant vaccines. The successful utilization of CRISPR/Cas9 in editing viral genomes has paved the way of developing novel and multiplex viral vectored poultry vaccines. Furthermore, CRISPR/Cas9 can be exploited to rectify major limitations of conventional approaches including reversion to virulent form, recombination with field viruses and transgene, and genome instability. This review provides comprehensive analysis of the potential of CRISPR/Cas9 genome editing technique in understanding avian virus-host interactions and developing novel poultry vaccines. Finally, we discuss the simplest and practical aspects of genome editing approaches in generating multivalent recombinant poultry vaccines that conform simultaneous protection against major avian diseases.

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Section: Introductionmentioning

confidence: 99%