Molecular Characterization, Antimicrobial Resistance and Caco-2 Cell Invasion Potential of Campylobacter jejuni/coli from Young Children with Diarrhea

Pan, Hongyu; Ge, Yigong; Xu, Hao; Zhang, Jianmin; Kuang, Dai; Yang, Xiaowei; Su, Xudong; Huang, Zheng; Shi, Xianming; Xu, Xuebin; Meng, Jianghong

doi:10.1097/inf.0000000000001016

Cited by 19 publications

(20 citation statements)

References 39 publications

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“…strains from ducks, milk, chickens, pork, and beef (Strachan and others ; Stone and others ; Bianchini and others ; Carrique‐Mas and others ; Noormohamed and Fakhr ; Revez and others ; Wei and others ). This tool is commonly used to determine the STs of Campylobacter isolates as well as genotypes and genetic diversity (Strachan and others ; Stone and others ; Bianchini and others ; Revez and others ; Wei and others ; Vidal and others ) and for typing (Behringer and others ; Abley and others ; Egger and others ; Manfreda and others ; Pan and others ; Vinueza‐Burgos and others ). MLST can be applied to determine macrolide and quinolone resistance based on partial sequences of 23S rRNA and gyr A genes (Jonas and others ).…”

Section: Molecular Tools Used For Typing Campylobacter Spp In Foods mentioning

confidence: 99%

“…Thus, it is easier and faster to employ molecular biology techniques such as polymerase chain reaction (PCR) and sequencing for identification purposes. In addition, tools such as pulsed‐field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) are the currently the most commonly used for typing (Goering ; Miller and others ; Behringer and others ; Gharst and others ; Manfreda and others ; Pan and others ; Vidal and others ; Oh and others ; Schleihauf and others ; Vinueza‐Burgos and others ; Zhang and others ).…”

Section: Introductionmentioning

confidence: 99%

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Molecular Detection, Typing, and Quantification of Campylobacter spp. in Foods of Animal Origin

Frasão

Marin

Conté-Júnior

2017

Comp Rev Food Sci Food Safe

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Abstract:The most frequently reported zoonosis and the main bacterial foodborne disease infection in humans is caused by Campylobacter spp., and C. jejuni and C. coli are the most common types. These bacteria can be found in the intestinal tracts of cattle, dogs, cats, sheep, poultry and pigs. The isolation of this microorganism is laborious because it requires specific media and a low oxygen concentration for growth. Additionally, differentiation between species through conventional bacteriology is difficult, as there are few different biochemical characteristics among the various species. Molecular microbiological techniques have become more important and are now broadly applied to help overcome difficulties in the identification, differentiation, and quantification of this pathogen. To date, there have been advances in the development and use of molecular techniques for the identification of microorganisms in foodstuffs. Tools such as pulsed-field gel electrophoresis and multilocus sequence typing are the most commonly used for typing. For the identification and confirmation of species, polymerase chain reaction (PCR) is crucial. Quantification by real-time PCR has wide applicability. To identify strains and antimicrobial resistance genes, sequencing technologies have been applied. This review builds on the discussion about the main and most widely used molecular methods for Campylobacter, as well as methods showing better potential for the classification, identification, and quantification of this important pathogen.

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Section: Molecular Tools Used For Typing Campylobacter Spp In Foods mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Detection, Typing, and Quantification of Campylobacter spp. in Foods of Animal Origin

Frasão

Marin

Conté-Júnior

2017

Comp Rev Food Sci Food Safe

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“…The major pathogens of the Campylobacter species in humans is Campylobacter jejuni , Campylobacter coli , Campylobacter lari , and Campylobacter fetus [ 10 ]. The group with the highest incidence rate of Campylobacter species infection is infants younger than 2 years of age [ 11 ]. Campylobacter species’ infection causes moderate to severe diarrhea in infants and young children [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…The group with the highest incidence rate of Campylobacter species infection is infants younger than 2 years of age [ 11 ]. Campylobacter species’ infection causes moderate to severe diarrhea in infants and young children [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Class 1 integrons and plasmid-mediated multiple resistance genes of the Campylobacter species from pediatric patient of a university hospital in Taiwan

et al. 2017

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BackgroundThe Campylobacter species usually causes infection between humans and livestock interaction via livestock breeding. The studies of the Campylobacter species thus far in all clinical isolates were to show the many kinds of antibiotic phenomenon that were produced. Their integrons cause the induction of antibiotic resistance between bacterial species in the Campylobacter species.ResultsThe bacterial strains from the diarrhea of pediatric patient which isolated by China Medical University Hospital storage bank. These isolates were identified by MALDI-TOF mass spectrometry. The anti-microbial susceptibility test showed that Campylobacter species resistant to cefepime, streptomycin, tobramycin and trimethoprim/sulfamethoxazole (all C. jejuni and C. coli isolates), ampicillin (89% of C. jejuni; 75% of C. coli), cefotaxime (78% of C. jejuni; 100% of C. coli), nalidixic acid (78% of C. jejuni; 100% of C. coli), tetracycline (89% of C. jejuni; 25% C. coli), ciprofloxacin (67% of C. jejuni; 50% C. coli), kanamycin (33% of C. jejuni; 75% C. coli) and the C. fetus isolate resisted to ampicillin, cefotaxime, nalidixic acid, tetracycline, ciprofloxacin, kanamycin by disc-diffusion method. The effect for ciprofloxacin and tetracycline of the Campylobacter species was tested using an E-test. The tet, erm, and integron genes were detected by PCR assay. According to the sequencing analysis (type I: dfr12-gcuF-aadA2 genes and type II: dfrA7 gene), the cassette type was identified. The most common gene cassette type (type I: 9 C. jejuni and 2 C. coli isolates; type II: 1 C. coli isolates) was found in 12 class I integrase-positive isolates.ConclusionsOur results suggested an important information in the latency of Campylobacter species with resistance genes, and irrational antimicrobial use should be concerned.Electronic supplementary materialThe online version of this article (doi:10.1186/s13099-017-0199-4) contains supplementary material, which is available to authorized users.

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“…Moreover, the extensive use of antibiotics in the poultry industry has led to the increasing prevalence of antibiotic-resistant C. jejuni strains. Some isolates are especially resistant to clinically used antibiotics, such as fluoroquinolones (FQ), which have been commonly used to treat acute bacterial diarrhea (3,5,6). Therefore, surveillance of the FQ susceptibility of C. jejuni is important for not only the purpose of animal breeding but also public health (7,8).…”

mentioning

confidence: 99%

Correlation between gyrA and CmeR Box Polymorphism and Fluoroquinolone Resistance in Campylobacter jejuni Isolates in China

Zhāng

Cheng

Luo

et al. 2017

Antimicrob Agents Chemother

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Sequence analysis of 79 ciprofloxacin-resistant Campylobacter jejuni isolates collected in China showed resistance-related sequence variations in gyrA and CmeR-Box. All the isolates contain an identical Thr-86-Ile substitution in GyrA. Several novel CmeR-Box variations, including point substitutions, deletion, and insertion, were identified. The point insertion or deletion led to dramatically reduced binding of CmeR to the cmeABC promoter, which significantly increases the expression of cmeABC and contributes to the high fluoroquinolone resistance.

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Molecular Characterization, Antimicrobial Resistance and Caco-2 Cell Invasion Potential of Campylobacter jejuni/coli from Young Children with Diarrhea

Abstract: Our findings provided baseline data on Campylobacter among young children. Active surveillance of Campylobacter is needed to better understand the epidemiology and antimicrobial resistance trends of this significant pathogen to help control and protect young children from such infections.

Cited by 19 publications

References 39 publications

Molecular Detection, Typing, and Quantification of Campylobacter spp. in Foods of Animal Origin

Molecular Detection, Typing, and Quantification of Campylobacter spp. in Foods of Animal Origin

Class 1 integrons and plasmid-mediated multiple resistance genes of the Campylobacter species from pediatric patient of a university hospital in Taiwan

Correlation between gyrA and CmeR Box Polymorphism and Fluoroquinolone Resistance in Campylobacter jejuni Isolates in China

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