Molecular characterization, expression pattern and association analysis of the porcine BTG2 gene

Abstract

B-cell translocation gene 2 (BTG2), a member of the B-cell translocation gene family with anti-proliferative properties, have been characterized to be involved in cell growth, differentiation and survival. In this study, we cloned the full length sequences of cDNA and genomic DNA of BTG2 gene from the porcine skeletal muscle. Spatial expression analysis showed that the porcine BTG2 gene is expressed predominantly in muscle. Temporal expression analysis in longissimus dorsi muscle demonstrated that the expression of BTG2 gene has the highest expression at 60 days old in Large White while with a peak expression at 120 days old in Meishan. Temporal analysis also revealed that the expression of BTG2 gene is generally higher in Large White than in Meishan at all the developmental stages tested (65 days of conception and 3, 35, 60, 120, and 180 days of postnatal). A single nucleotide polymorphism (G417C) in the intron of BTG2 gene was then detected by PCR-RFLP in Large White × Meishan F2 resource population and association analysis suggested that this polymorphic site had significant association (P < 0.05) with the buttock fat thickness, fat percentage, lean muscle percentage, ratio of lean to fat and carcass length.

Molecular genetics-nutrition interactions in the expression of AANAT, ADRB3, BTG2 and FASN genes in the heart, kidney and liver of Australian lambs supplemented with Spirulina (Arthrospira platensis)

“…BTG2 belongs to the anti-proliferative gene family and has been shown to be involved in cell growth, differentiation and survival (Mo et al 2011), muscle fibre size, intramuscular fat deposition and weight loss (Kamaid and Giráldez 2008;Sasaki et al 2006). FA analyses demonstrated that x-3 and x-6 FAs increased in the liver of lambs supplemented at 10 and 20 % Spirulina levels.…”

Gene Expression Profiles of Aralkylamine N-Acetyltransferase, B-cell Translocation Gene-2 and Fatty Acid Synthase in Pasture-based Primiparous Holstein-Friesian Dairy Cows Supplemented with Crude Degummed Canola Oil

“…The protein encoded by this gene controls cell cycle progression and proneural gene expression by acting as a transcription co-regulator that enhances or inhibits the activity of transcription factors [23,24]. Thus, BTG2 has many functions involving regulation of cell growth, death, differentiation and survival [25].…”

Polymorphism, linkage mapping, and association analysis with carcass traits of four porcine candidate genes selected from gene-expression profiles of Czech Large White and Wild Boar muscles

“…In the homologous segment of human chromosome 11q23.3, there is a cluster of several apolipoprotein genes (APOA1, APOA4, APOA5, and APOC3) (http://www.ensembl.org/), of which some (APOA1, APOA4) are involved in fat digestion and absorption. Mo et al (2011) performed association analysis of an intronic BTG2 polymorphism with carcass traits in a Large White × Meishan F 2 resource population and found associations with buttock fat thickness, fat percentage, lean meat percentage, ratio of lean to fat, and carcass length. In an F 2 population created by crossing Pietrain, Large White, Landrace, and Leicoma, Duthie et al (2008) identified several QTL at a position around 90 cM on SSC9, where BTG2 may be located.…”

“…Minor QTL for growth and fattening traits were identified in this region in a Wild Boar × Pietrain F 2 family (Cepica et al, 2003a). BTG2, due to its biological functions (Mo et al, 2011), may be a candidate for some growth and carcass traits.…”

“…Feng et al (2007) studied mRNA expression levels of porcine BTG2 and BTG3 genes in proliferating murine myoblast cells and concluded that the two genes may be important for skeletal muscle growth. BTG2 is expressed at high levels in porcine foetal and adult skeletal muscle tissue (Feng et al, 2007), and different breeds differ in the expression level -Large White has a higher expression compared to Meishan (Mo et al, 2011). Porcine BTG2 was mapped by radiation hybrid mapping to SSC9, and the most likely chromosomal location is 9q2.1-q2.5 (Feng et al, 2007).…”