Molecular Characterization of 28 Mango Germplasm Using RAPD

Rahman, M. Mizanur; Rabbani, M. G.; Siddique, M. N. A.; Garvey, E. J.; Rahaman, Ebna Habib Md Shofiur

doi:10.3329/ptcb.v17i1.1123

Cited by 15 publications

(6 citation statements)

References 7 publications

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“…The fifteen primers generated 177 bands with sizes ranged from 100 -5000 bp (Table 1). This was comparable with the results ranging from 200 bp to 3000 bp (Kumar et al, 2001), 190 bp to 2400 bp (Karihaloo et al, 2003), 105 bp to 1500 bp (Rahman et al, 2007), 250 bp to 2500 bp (Anuj et al, 2007), 240 bp to 2100 bp (Ahmad et al, 2008), 300 bp to 3500 bp (Bajpai et al, 2008), 100 bp to 3000bp (Das et al, 2010) and 70 bp to 4749 bp (Gajera et al, 2011) in mango.…”

Section: Resultssupporting

confidence: 89%

Molecular characterization of traditional mango germplasm of coastal Andhra Pradesh using RAPD markers

2021

EJPB

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A study was conducted at Horticultural research station, Venkataramannagudem for evaluating the variability of mango germplasm to conserve the elite ones and to identify the superior genotypes using molecular markers for future crop improvement. Genetic variation and relationship among 34 traditional mango germplasm were analyzed using Random Amplified Polymorphic DNA (RAPD) markers. Fifteen out of the twenty primers screened were informative and 177 amplified DNA bands with sizes ranging from 100 -5000 bp were selected as RAPD markers. The number of amplified fragments varied from 3 (OPG 13) to 20 (OPX 04) with an average of 11.8 polymorphic fragments per marker. Specific RAPD markers for some mango germplasm were identified. Unweighted Pair Group Method using Arithmetic means (UPGMA) cluster analysis grouped all the germplasm into two clusters with a genetic similarity coefficient range of 0.67 to 0.88. The accessions within the germplasm i.e., Banganapalli -1, Banganapalli -2 and Banginapalli-3 showed genetic similarity which is in good agreement from a single ancestor with their putative pedigrees. The genotypes Panukula Mamidi and Rajamamidi were closely clustered since these two genotypes are native to the Vizianagaram district. Similarly, the genotypes Nuzividu Tiyya Mamidi and Nuzividu Rasalu occurred in the same cluster indicated that these genotypes also originated from the same geographical area, it can be concluded that they were separated into the cluster based on geographic origin. This study showed clearly that germplasm from coastal Andhra Pradesh unveiled maximum diversity and indicated the potential of RAPD markers for the identification of mango germplasm for breeding purposes.

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Section: Resultssupporting

confidence: 89%

Molecular characterization of traditional mango germplasm of coastal Andhra Pradesh using RAPD markers

2021

EJPB

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“…Relatively higher number of alleles (Na) detected in the present study in comparison to the earlier reports may be attributed to relatively large and diverse accessions used. Different levels of polymorphism ranging from 73 to 100 % using markers like RAPD (Ravishankar et al (2000); Karihaloo et al (2003); Rahman et al (2007); Rajwana et al (2008); Souza et al (2011), ISSR polymorphism ranging from 85 to 97 % (Pandit et al (2007); Samal et al (2012); Tomar et al (2011) AFLP ranging from 84 to 96 % (Yamanaka et al (2006); Gálvez-López et al (2010) and 73 % of polymorphism by SCoT markers (Luo et al 2010) have been reported in earlier studies involving mango.…”

Section: Ssr Diversitymentioning

confidence: 88%

“…Molecular markers on the other hand have proved their potentiality in unraveling the limitations of morphological traits. In recent years, extensive molecular work has been carried out to estimate the genetic variation in mango germplasm using random amplified polymorphic DNA (RAPD) markers (Schnell et al 1995;Lopez et al 1997;Ravishankar et al 2000;Hemant Kumar et al 2001;Karihaloo et al 2003;Rahman et al 2007;Rajwana et al 2008;Díaz-Matallana et al 2009;Souza et al 2011;Ramessur and Ranghoo 2011) and restriction fragment length polymorphism (RFLP) analysis (Eiadthong et al 1999); amplified fragment length polymorphism (AFLP) markers (Eiadthong et al 2000;Kashkush et al 2001;Yamanaka et al 2006;Gálvez-López et al 2010) inter simple sequence repeat (ISSR) markers (He et al 2005;Pandit et al 2007;Srivastava et al 2007;Tomar et al 2011;Rocha et al 2012;Samal et al 2012) and start codon targeted (SCoT) markers (Luo et al 2010). Among the molecular markers simple sequence repeat or (microsatellites) have been found to be the marker of choice for more precisely understanding the genetic diversity, gene mapping, and cultivar discrimination due to their abundance, co-dominant and high reproducibility nature (Gupta and Varshney 2000).…”

Section: Introductionmentioning

confidence: 99%

Population structure and genetic analysis of different utility types of mango (Mangifera indica L.) germplasm of Andhra Pradesh state of India using microsatellite markers

Malathi

Vemireddy

Begum³

et al. 2013

Plant Syst Evol

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Genetic analysis of 90 mango genotypes including juicy, table, dual and pickle types from different parts of Andhra Pradesh of India was carried out employing 143 mango-specific microsatellite markers. Of the 143, 34 were new mango-specific microsatellite loci isolated in the course of the present investigation by constructing an (CA) n and (TG) n -enriched genomic library. Characterization of the 90 genotypes resulted in the detection of 301 alleles from 106 polymorphic loci with an average of 2.87 alleles per locus and polymorphism information content of 0.67. UPGMA cluster analysis grouped all the genotypes into two major groups with a genetic similarity range of 47-88 %. Grouping of the genotypes based on the utility type was observed only at sub-cluster level. Study of population structure by a model-based STRUCTURE analysis revealed the germplasm to exist in four gene pools. Overall F st of 0.11 indicated genetic differentiation between the populations to be low. Analysis of molecular variance revealed that major proportion of the variation was within the individuals (62.25 %). The molecular marker-based study of genetic diversity suggests that the germplasm studied representing the kind of variability would be a valuable genetic resource for future breeding and association mapping in search for new and novel alleles.

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“…The high level of heterozygosity in our study was attributed to cross pollination and out crossing. Different levels of genetic diversity and polymorphism have been reported in mango using various types of markers, like 90% and above by SSR markers (Dillon et al, 2013;Surapaneni et al, 2013), 85 to 99% with ISSR (Ariffin et al, 2015;Samal et al, 2012;Tomar et al, 2011), 73 to 100% with RAPD (Ravishankar et al, 2000;Karihaloo et al, 2003;Rahman et al, 2007;Rajwana et al, 2008;Souza et al, 2011), 84 to 96% by AFLP (Yamanaka et al, 2006;Ga´lvez-Lo´pez et al, 2010) and 73% by SCoT markers (Luo et al, 2010). The average PIC value obtained in this study was 0.764 which was higher than the average value reported by earlier studies (Surapaneni et al, 2013;Dillon et al, 2013;Ravishankar et al, 2011;Hirano et al, 2010 andSchnell et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of Wild and Cultivated Mango Genotypes of Pakistan Using SSR Markers

2018

PAKJAS

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Pakistan is blessed with a wide range of indigenous mango germplasm. Wild mango genotypes, growing at Azad Jammu and Kashmir (AJK) and its vicinity are valuable resource for unique genetic diversity. The DNA fingerprints of this available germplasm have never been worked out. Hence, the aim of this study was to develop DNA profiles of 31 wild and 13 cultivated genotypes of the country to determine the population structure. Number of alleles per locus of the 51 Simple sequence repeat (SSR) markers ranged from 3 to 9 and a total of 296 alleles with an average of 5.80 alleles per locus. The average polymorphism information content value was 0.764. The expected and observed heterozygosity values were 0.805 and 0.720, respectively, which exhibited high level of genetic diversity in the wild and cultivated mango germplasm. The Bayesian cluster, principal coordinate and hierarchical clustering analyses divided the collected genotypes into three groups i.e. A, B and C. Members of group A and B consisted of wild genotypes entirely, while all commercial genotypes were clustered in group C. The obtained results highlighted genetic diversity encompassed by wild mango genotypes of AJK which can be considered as distinct genotypes for further evaluations in the framework of breeding programs and new cultivar identification in mango.

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Molecular Characterization of 28 Mango Germplasm Using RAPD

Cited by 15 publications

References 7 publications

Molecular characterization of traditional mango germplasm of coastal Andhra Pradesh using RAPD markers

Molecular characterization of traditional mango germplasm of coastal Andhra Pradesh using RAPD markers

Population structure and genetic analysis of different utility types of mango (Mangifera indica L.) germplasm of Andhra Pradesh state of India using microsatellite markers

Genetic Diversity of Wild and Cultivated Mango Genotypes of Pakistan Using SSR Markers

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