Molecular characterization of a Babesia gibsoni isolate from a Spanish dog

Criado–Fornelio, A.; Gónzalez-del-Rı́o, M.A.; Buling-Saraña, A.; Barba-Carretero, J.C.

doi:10.1016/j.vetpar.2003.08.006

Cited by 54 publications

(23 citation statements)

References 16 publications

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“…Several studies have detected this species in dogs from Hungary, Croatia, Italy, Poland, Spain, Switzerland, Portugal, Russia, Germany, Slovenia, France, Albania, the Netherlands, Norway (Solano-Gallego and Baneth, 2011) and Romania (Ionita et al, 2012). Babesia gibsoni infection has been described in some European countries using molecular methods: two dogs in Germany (Hartelt et al, 2007), four in Spain (Criado-Fornelio et al, 2003;Tabar et al, 2009), one dog in Italy (Trotta et al, 2009), 14 dogs in Romania (Imre et al, 2013) and six from Croatia were diagnosed with B. gibsoni subclinical infection (Beck et al, 2009). Our results support previous findings reported from neighbouring countries that B. canis is considered the main species which causes canine babesiosis in this part of Europe.…”

Section: Discussionmentioning

confidence: 99%

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Clinical babesiosis and molecular identification of Babesia canis and Babesia gibsoni infections in dogs from Serbia

Davitkov

Vučićević

Stevanović

et al. 2015

Acta Veterinaria Hungarica

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Canine babesiosis is a frequent and clinically significant tick-borne disease. Sixty symptomatic dogs with clinical findings compatible with babesiosis were included in this study conducted in Serbia. After clinical examination, blood samples were taken for microscopic examination, complete blood count (CBC), Canine SNAP 4Dx Test, DNA analyses and sequencing. The main clinical signs included apathy, anorexia, fever, brown/red discoloration of urine, pale mucous membranes, icterus, splenomegaly, and vomiting. The main clinicopathological findings in Babesia infections were a slight to severe thrombocytopenia and a mild to very severe normocytic normochromic anaemia. Microscopic evaluation revealed 58 positive samples with the presence of large and small intraerythrocytic piroplasms in 57 and 1 sample(s), respectively. No co-infections were found using SNAP test. Two Babesia species, B. canis (58/60) and B. gibsoni (2/60), were differentiated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Species identification was further confirmed by sequencing PCR products of B. gibsoni samples and six randomly selected B. canis samples. All dogs were treated with imidocarb dipropionate (6.6 mg/kg of body weight), given intramuscularly twice at an interval of 14 days. This report presents the first molecular evidence of the occurrence of B. gibsoni and B. canis, confirmed by DNA sequencing, in sick dogs from Serbia.

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Section: Discussionmentioning

confidence: 99%

“…However, some Babesia species that lead to different clinical manifestations cannot be morphologically distinguished (Kjemtrup et al, 2000;Jefferies et al, 2007). These Babesia species can be differentiated by molecular methods and not by the blood smear method (Criado-Fornelio et al, 2003). Moreover, molecular methods have other significant advantages, i.e.…”

mentioning

confidence: 99%

Clinical babesiosis and molecular identification of Babesia canis and Babesia gibsoni infections in dogs from Serbia

Davitkov

Vučićević

Stevanović

et al. 2015

Acta Veterinaria Hungarica

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“…It allows one to demonstrate the presence of Babesia genetic material in parasitemia at levels of only 0.0001% (Ano et al 2001). Moreover, with its help, it is possible to differentiate those parasites which do not show an altered cellular morphology (Criado-Fornelio et al 2003). The sensitivity of this technique depends on the amount of template DNA, and that amount depends, in turn, on the efficiency of the method of nucleic acid isolation (McPherson et al 1991).…”

Section: Discussionmentioning

confidence: 99%

Identification of new 18S rRNA strains of Babesia canis isolated from dogs with subclinical babesiosis

Łyp¹,

Adaszek²,

Furmaga³

et al. 2015

Polish Journal of Veterinary Sciences

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In this study, we used PCR to detect and characterize B. canis from naturally infected dogs in Poland with subclinical babesiosis by amplifying and sequencing a portion of the 18S ribosomal RNA (rRNA) gene. Venous blood samples were collected from ten dogs with subclinical babesiosis. A 559-bp fragment of the B. canis 18S rRNA gene was amplified by PCR. Sequencing of the PCR products led to the identification of a new variant of Babesia canis, differing from the previously detected protozoa genotypes (18S rRNA-A and 18S rRNA-B) with nucleotide substitutions in positions 150 and 151 of the tested gene fragment. The results indicate the emergence within the Polish territory of a new, previously unencountered Babesia canis genotype responsible for the development of subclinical babesiosis.

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“…PCR and sequencing of positive samples have proved to be highly sensitive and specific, and are important in establishing a definitive diagnostic method, since the clinical signs involved in hemoparasite infections are clinically very similar (CRIADO-FORNELIO et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Rangelia vitalii, Babesia spp. and Ehrlichia spp. in dogs in Passo Fundo, state of Rio Grande do Sul, Brazil

Gottlieb

André

Soares³

et al. 2016

Rev. Bras. Parasitol. Vet.

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Pathogens transmitted by ticks are an emerging problem worldwide, this study aimed to diagnose the causal agents of infection in dogs presenting suspected hemoparasitoses. Fifty-eight dogs with clinical signs such as depression, hemorrhagic diathesis and fever were evaluated regarding clinical presentation, hemogram, blood smears and serological tests, using the indirect immunofluorescence method for the agents Babesia vogeli and Ehrlichia canis and conventional PCR for Babesia spp. (gene 18S rRNA), Rangelia vitalii (gene 18S rRNA) and Ehrlichia spp. (gene dsb). Five (8.6%) of the 58 dogs were serologically positive for Babesia spp. and three (5.1%) for E. canis. Four dogs (6.8%) were positive for R. vitalii through the molecular diagnosis. The PCR products were sequenced and the DNA from R. vitalii was found to be 99% genetically identical to samples of R. vitalii that had been isolated in Brazil. No presence of Babesia spp. or E. canis was observed through PCR on the dogs evaluated here. The results indicate the presence of R. vitalii and exposure to Babesia spp. and Ehrlichia spp. among the dogs analyzed.Keywords: Hemoparasites, piroplasms, sequencing, dogs, nambiuvú, indirect immunofluorescence. ResumoPatógenos transmitidos por carrapatos são um problema emergente em todo o mundo, o trabalho objetivou diagnosticar os agentes causais da infecção em cães com suspeita de hemoparasitoses. Cinquenta e oito caninos com sinais clínicos como depressão, diáteses hemorrágicas e febre foram avaliados quanto à apresentação clínica, hemograma, esfregaço sanguíneo, sorologia pelo método de Imunofluorescência Indireta para os agentes Babesia vogeli e Ehrlichia canis e na PCR convencional para Babesia spp. (gene 18S rRNA), Rangelia vitalii (gene 18S rRNA) e Ehrlichia spp. (gene dsb). Cinco (8,6%) dos 58 cães apresentaram sorologia positiva para Babesia spp. e três (5,1%) para E. canis. Quatro (6,8%) animais mostraram-se positivos para R. vitalii no diagnóstico molecular. Os produtos da PCR foram sequenciados e o DNA encontrado de R. vitalii mostrou 99% de identidade genética com amostras de R. vitalii isoladas no Brasil. Não foi observada a presença de Babesia spp. e E. canis na PCR dos cães avaliados. Os resultados indicaram a presença de R. vitalii e exposição a Babesia spp. e Ehrlichia spp. entre os cães analisados.

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Molecular characterization of a Babesia gibsoni isolate from a Spanish dog

Cited by 54 publications

References 16 publications

Clinical babesiosis and molecular identification of Babesia canis and Babesia gibsoni infections in dogs from Serbia

Clinical babesiosis and molecular identification of Babesia canis and Babesia gibsoni infections in dogs from Serbia

Identification of new 18S rRNA strains of Babesia canis isolated from dogs with subclinical babesiosis

Rangelia vitalii, Babesia spp. and Ehrlichia spp. in dogs in Passo Fundo, state of Rio Grande do Sul, Brazil

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