Molecular characterization of a Brugia malayi transglutaminase

Devarajan, Eswaran; Mishra, Pankaj Kumar; Thirugnanam, S.; Mehta, Kapil; Chandrashekar, Ramaswamy; Kaliraj, Perumal

doi:10.1007/s00436-004-1121-9

Cited by 6 publications

(2 citation statements)

References 25 publications

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“…Recently, a homolog of TG having significant homology to PDI and thioredoxin active sites was cloned and characterized at the protein level from nematode B. malayi [45]. Preliminary studies suggest that individuals living in areas, endemic for filarial infections (endemic normals) harbor high titers of anti-TG antibodies [45], implicating a possible role for TG in mounting protective immune response in the human host against filarial parasites.…”

Section: Gene Sequence Analysis Of Tgsmentioning

confidence: 98%

“…Preliminary studies suggest that individuals living in areas, endemic for filarial infections (endemic normals) harbor high titers of anti-TG antibodies [45], implicating a possible role for TG in mounting protective immune response in the human host against filarial parasites. These initial observations warrant further evaluation of TG as a target for developing effective vaccine and/or chemotherapeutic agents for controlling infections caused by these nematode parasites.…”

Section: Gene Sequence Analysis Of Tgsmentioning

confidence: 99%

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Transglutaminases of Lower Organisms

Rao

Chandrashekar²,

Mehta³

2005

Transglutaminases

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Section: Gene Sequence Analysis Of Tgsmentioning

confidence: 98%

Section: Gene Sequence Analysis Of Tgsmentioning

confidence: 99%

Transglutaminases of Lower Organisms

Rao

Chandrashekar²,

Mehta³

2005

Transglutaminases

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Molecular characterization and evaluation of Onchocerca volvulus-secreted larval acidic protein 1 (SLAP1) as a putative vaccine candidate on endemic population of lymphatic filariasis

et al. 2013

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Filarial parasites infected nearly 160 million of the global population with onchocerciasis and lymphatic filariasis, and further, a billion of people are estimated to be at risk of infection, rendering them among the most prevalent infectious agents in the world today. Given the complexity of their life cycle and the immune evasion mechanisms of these organisms, development of a vaccine remains to be a long-term challenge. Though a number of immunodominant antigens have been characterized, the presence of homologous proteins in humans or the allelic variants are some of the major drawbacks. One of the extensively studied vaccine candidates is abundant larval transcripts (ALT) family of proteins for the following properties: highly regulated expression, abundance, excreted-secreted product of infective stage larvae, and essentially for parasite establishment and survival in the host. In the present study, stage-specific expression of secreted larval acidic protein 1 (SLAP1) was identified; an ALT orthologue from Onchocerca volvulus was cloned, expressed, and purified as a recombinant protein. Immunogenicity of OvSLAP1 was demonstrated with sera and peripheral blood mononuclear cells from endemic regions of Brugia malayi and Wuchereria bancrofti. OvSLAP1 antibodies were predominated by IgG1 and IgG2 in endemic normal (EN) and chronic pathology (CP) subjects. It has also induced marked cellular response as observed by lymphoproliferation assay. The study revealed that OvSLAP1 can segregate humoral (EN mean optical density (OD) = 0.87 ± 0.035, CP mean OD = 0.59 ± 0.029) and cellular (EN mean stimulation index (SI) = 5.87 ± 0.167, CP mean SI = 3.5 ± 0.134) immune responses between EN and CP individuals (P < 0.001), signifying its prophylactic ability and vitality for protection from filarial infections in endemic population.

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Immunoprophylaxis of multi-antigen peptide (MAP) vaccine for human lymphatic filariasis

et al. 2017

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Human lymphatic filariasis, the parasitic disease caused by the filarial nematodes Wuchereria bancrofti, Brugia malayi, and Brugia timori, is ranked as the second most complex clinical condition leading to permanent and long-term disability. The multiple antigen peptide (MAP) approach is an effective method to chemically synthesize and deliver multiple T and B cell epitopes as the constituents of a single immunogen. Here, we report on the design, chemical synthesis, and immunoprophylaxis of three epitopes that have been identified from promising vaccine candidates reported in our previous studies, constructed as MAP on an inert lysine core for human lymphatic filariasis in Jird model. Two epitopes from Thioredoxin and one epitope from Transglutaminase were constructed as MAP in an inert lysine core. The immunoprophylaxis of the synthetic vaccine construct studied in Jird models showed protective antibody (1 in 64,000 titer) and cellular immune response. Thioredoxin-Transglutaminase MAP (TT MAP) conferred a significantly high protection of 63.04% compared to control (8.5%). Multi-antigen peptide vaccine is one best approach to provide immunity against multiple antigens delivered by the complex filarial parasite.

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Molecular characterization of a Brugia malayi transglutaminase

Cited by 6 publications

References 25 publications

Transglutaminases of Lower Organisms

Transglutaminases of Lower Organisms

Molecular characterization and evaluation of Onchocerca volvulus-secreted larval acidic protein 1 (SLAP1) as a putative vaccine candidate on endemic population of lymphatic filariasis

Immunoprophylaxis of multi-antigen peptide (MAP) vaccine for human lymphatic filariasis

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