2013
DOI: 10.1016/j.aoas.2013.01.014
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Molecular characterization of a cucumber mosaic cucumovirus isolated from lettuce in Egypt

Abstract: Cucumber mosaic cucumovirus (CMV) was isolated from lettuce plants (Lactuca sativa)showing virus like symptoms. Isolation was performed depending on specific polyclonal antibodies and Chenopodium quinoa as a local lesion host. Virus was purified from 200 gm of virus-infected Nicotiana tabacum cv. White Burley leaves giving A 260/280 ratio of 1.21 and a yield of 1.7 mg. Purified virus preparation was used for rabbit immunization to produce specific polyclonal antibodies. IgGs were purified and evaluated by indi… Show more

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Cited by 6 publications
(6 citation statements)
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“…Cucumber mosaic virus (CMV) is one of the most widespread plant viruses with an extensive host range, infecting approximately 1,000 species in over 100 plant families that include cereals, fruits, vegetables and ornamentals, and can cause significant economic losses for numerous vegetable and horticultural crops (Roossinck, 1999). In lettuce plants symptoms caused by CMV include plant stunting, leaf chlorosis, and mosaic as well as poor head formation (Bruckart and Lorbeer, 1975;El-Borollosy and Waziri, 2013). The virus is readily transmitted in a non-persistent manner by more than 75 species of aphids (Palukaitis et al, 1992).…”
mentioning
confidence: 99%
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“…Cucumber mosaic virus (CMV) is one of the most widespread plant viruses with an extensive host range, infecting approximately 1,000 species in over 100 plant families that include cereals, fruits, vegetables and ornamentals, and can cause significant economic losses for numerous vegetable and horticultural crops (Roossinck, 1999). In lettuce plants symptoms caused by CMV include plant stunting, leaf chlorosis, and mosaic as well as poor head formation (Bruckart and Lorbeer, 1975;El-Borollosy and Waziri, 2013). The virus is readily transmitted in a non-persistent manner by more than 75 species of aphids (Palukaitis et al, 1992).…”
mentioning
confidence: 99%
“…Viral disease management is dependent upon an accurate detection procedure which is convenient, reproducible and scalable for a wide range of samples (Viswanathan et al, 2013). Currently available methods commonly used for detecting viruses that affect lettuce include enzyme-linked immunosorbent assay (ELISA), reverse transcription (RT)polymerase chain reaction (PCR) for LNYV and CMV (Callaghan and Dietzgen, 2005;Deyong et al, 2005;Dietzgen and Francki, 1988;Koenig, 1981) and immunocapture (IC)-RT-PCR for CMV (El-Borollosy and Waziri, 2013). These methods are valuable for identifying latent infections at most stages of infection.…”
mentioning
confidence: 99%
“…al., 2013). Specificity and efficiency of transmission of CMV strains by species of aphids has been mapped to several domains in the amino acid sequence of the CP (Quemada, et al, 1989, Perry, et al, 1998and El-Borollosy & Waziri, 2013. These domains could be directly involved in the binding of virus particles to aphid receptors or, alternatively, affect the stability of particles in the aphid stylet and foregut (Quemada, et al, 1989).…”
Section: Introductionmentioning
confidence: 99%
“…CMV subgroup I has been recently divided into IA and IB on the basis of gene sequences available for CMV strains and phylogenetic analysis (Roossinck, 2002). In Egypt, almost all isolates of CMV studied so far (Sofy and Soliman, 2011;Megahed et al, 2012;El-Borollosy and Waziri, 2013;Nour El-Din et al, 2013;Rabie et al, 2017) were found to belong to either subgroup IA or IB. According to the available literature, no work has so far been reported on the isolation of a CMV belonging to subgroup II in Egypt.…”
Section: Introductionmentioning
confidence: 99%