Molecular characterization of a first begomovirus associated with lentil (Lens culinaris) from India

Abstract

Summary. -A disease of lentil with symptoms of distortion, mottling and chlorosis in the leaves, shortening of internodes and excessive branching was noticed in lentil at Kanpur, India, during 2012. Results of polymerase chain reaction and reverse transcriptase polymerase chain reaction employed to detect suspected RNA and DNA viruses indicated involvement of a geminivirus, which was further characterized by sequencing of full genome amplified by rolling circle amplification. Analysis of full length DNA-A revealed 96.4-96.7% nucleotide similarity with bitter gourd yellow vein virus (BGYVV) isolates and tomato leaf curl New Delhi virus (ToLCNDV) isolate. As per the recent revision of begomovirus species demarcation criteria, if a new virus isolate shares ≥91% nt sequence identity with any other isolate of an existing species, it should be treated as an isolate of that species, even if it is <91% identical to all other isolates from that species. This made BGYVV an isolate of ToLCNDV and resulted in the de-recognizing of the BGGYV.

First report of a begomovirus infecting Ocimum kilimandscharicum in India

“…The PCR products were cloned and sequenced. BlastN analysis revealed 97.6% identity with the DNA-A segment of Tomato leaf curl New Delhi virus (ToLCNDV) from lentil isolated in Kanpur, India (Naimuddin et al, 2016). The sequence was submitted to GenBank (Accession no.…”

Molecular characterization of mungbean yellow mosaic India virus infecting Vigna radiata in Oman

“…MYMIV has been reported to infect different host plants: cucumber ( Cucumis sativus ), cowpea ( Vigna unguiculata ), mungbean ( Vigna radiata ), urdbean ( Vigna mungo ), lentil ( Lens culinaris ), tomato ( Solanum lycopersicum ), soya bean ( Glycine max ) and yard long bean ( V. unguiculata ssp. Sesquipedalis) (John et al., 2008; Maiti et al., 2011; Naimuddin et al., 2016; Ramesh et al., 2019; Shahid et al., 2019). Moreover, different betasatellites have been associated with MYMIV (Malathi et al., 2003; Varma et al., 2008).…”

Nightshade (Solanum nigrum), an intermediate host between tomato and cucurbits of Tomato leaf curl New Delhi virus

“…43 different plant species in the Indian subcontinent, West Asia, and Europe (Moriones et al, 2017). The virus affects plant species in the Cucurbitaceae, Fabaceae, Malvaceae, Euphorbiaceae, and Solanaceae (Hussain et al, 2004;Ito et al, 2008;Naimuddin et al, 2016). ToLC-NDV is an emerging virus in the Mediterranean basin.…”