Molecular characterization of a novel alternavirus infecting the entomopathogenic fungus Cordyceps chanhua

Zhang, Yuxiang; Shi, Najie; Wang, Ping; Zhu, Qiuyan; Yang, Guogen; Huang, Bo

doi:10.1007/s00705-022-05446-2

Cited by 14 publications

(19 citation statements)

References 16 publications

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“…Mycoviruses isolated from C. chanhua have not been investigated extensively. To date, only two viruses, one Alternavirus CcAV1 and one Victorivirus CcV1, have been described in C. chanhua [29]. In this study, we report a third novel dsRNA virus infecting C. chanhua.…”

Section: Discussionmentioning

confidence: 74%

A Novel Gammapartitivirus That Causes Changes in Fungal Development and Multi-Stress Tolerance to Important Medicinal Fungus Cordyceps chanhua

Zhu

Shi

Wang

et al. 2022

JoF

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Cicada flower, scientifically named Cordyceps chanhua, is an important and well-known Chinese cordycipitoid medicinal mushroom. Although most mycoviruses seem to induce latent infections, some mycoviruses cause host effects. However, the effects of mycovirus on the fungal development and stress tolerance of C. chanhua remain unknown. In this study, we report a novel mycovirus designated Cordyceps chanhua partitivirus 1 (CchPV1) from C. chanhua isolate RCEF5997. The CchPV1 genome comprises dsRNA 1 and dsRNA 2, 1784 and 1563 bp in length, respectively. Phylogenetic analysis using the aa sequences of RdRp revealed that CchPV1 grouped with members of the genus Gammapartitivirus in the family Partitiviridae. We further co-cultivated on PDA donor strain RCEF5997 and recipient C. chanhua strain RCEF5833 (Vf) for 7 days, and we successfully obtained an isogenic line of strain RCEF5833 with CchPV1 (Vi) through single-spore isolation, along with ISSR marker and dsRNA extraction. The biological comparison revealed that CchPV1 infection slows the growth rate of the host, but increases the conidiation and formation of fruiting bodies of the host. Furthermore, the assessment of fungal tolerance demonstrated that CchPV1 weakens the multi-stress tolerance of the host. Thus, CchPV1 infection cause changes in fungal development and multi-stress tolerance of the host C. chanhua. The findings of this study elucidate the effects of gammapartitivirus on host entomogenous fungi and provide a novel strategy for producing high-quality fruiting bodies of C. chanhua.

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Section: Discussionmentioning

confidence: 74%

A Novel Gammapartitivirus That Causes Changes in Fungal Development and Multi-Stress Tolerance to Important Medicinal Fungus Cordyceps chanhua

Zhu

Shi

Wang

et al. 2022

JoF

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“…In the present study, FaAV1 was extracted from F. avenaceum strain GS-WW-224 and identified to be a new alternavirus, which is the first time that an alternavirus has been identified in F. avenaceum . Thus far, eleven mycoviruses have been designated as members of the proposed family Alternaviridae [ 7 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. Among them, FiAV1 [ 7 ], FpAV1 [ 23 ], FgAV1 [ 24 ], AheAV1 [ 25 ], and CcAV1 [ 28 ] contain three dsRNA segments, while AsV341 [ 19 ], AaV1 [ 20 , 21 ], AfV [ 22 ], FoAV1 [ 26 ], FsAV1 [ 27 ], and SlAV1 [ 29 ] contain four dsRNA segments.…”

Section: Discussionmentioning

confidence: 99%

“…Thus far, eleven mycoviruses have been designated as members of the proposed family Alternaviridae [ 7 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ]. Among them, FiAV1 [ 7 ], FpAV1 [ 23 ], FgAV1 [ 24 ], AheAV1 [ 25 ], and CcAV1 [ 28 ] contain three dsRNA segments, while AsV341 [ 19 ], AaV1 [ 20 , 21 ], AfV [ 22 ], FoAV1 [ 26 ], FsAV1 [ 27 ], and SlAV1 [ 29 ] contain four dsRNA segments. The genomic composition of FaAV1 identified in the present study, however, only contains two dsRNA segments.…”

Section: Discussionmentioning

confidence: 99%

“…To date, eleven mycoviruses have been designated as alternaviruses in the proposed family Alternaviridae. These include Aspergillus mycovirus 341 (AsV341) [ 19 ], Alternaria alternata virus 1 (AaV1) [ 20 , 21 ], Aspergillus foetidus virus (AfV) [ 22 ], Fusarium poae alternavirus 1 (FpAV1) [ 23 ], Fusarium graminerarum alternairus 1 (FgAV1) [ 24 ], Aspergillus heteromorphus alternavirus 1 (AheAV1) [ 25 ], Fusarium incarnatum alternavirus 1 (FiAV1) [ 7 ], Fusarium oxysporum alternavirus 1 (FoAV1) [ 26 ], Fusarium solani alternavirus 1 (FsAV1) [ 27 ], Cordyceps chanhua alternavirus 1 (CcAV1) [ 28 ], and Stemphylium lycopersici alternavirus 1 (SlAV1) [ 29 ].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the First Alternavirus Identified in Fusarium avenaceum, the Causal Agent of Potato Dry Rot

Zhang

Hua

et al. 2023

Viruses

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A novel virus with a double-stranded RNA (dsRNA) genome was isolated from Fusarium avenaceum strain GS-WW-224, the causal agent of potato dry rot. The virus has been designated as Fusarium avenaceum alternavirus 1 (FaAV1). Its genome consists of two dsRNA segments, 3538 bp (dsRNA1) and 2477 bp (dsRNA2) in length, encoding RNA-dependent RNA polymerase (RdRp) and a hypothetical protein (HP), respectively. The virions of FaAV1 are isometric spherical and approximately 30 nm in diameter. Multiple sequence alignments and phylogenetic analyses based on the amino acid sequences of RdRp and HP indicated that FaAV1 appears to be a new member of the proposed family Alternaviridae. No significant differences in colony morphology and spore production were observed between strains GS-WW-224 and GS-WW-224-VF, the latter strain being one in which FaAV1 was eliminated from strain GS-WW-224. Notably, however, the dry weight of mycelial biomass of GS-WW-224 was higher than that of mycelial biomass of GS-WW-224-VF. The depth and the width of lesions on potato tubers caused by GS-WW-224 were significantly greater, relative to GS-WW-224-VF, suggesting that FaAV1 confers hypervirulence to its host, F. avenaceum. Moreover, FaAV1 was successfully transmitted horizontally from GS-WW-224 to ten other species of Fusarium, and purified virions of FaAV1 were capable of transfecting wounded hyphae of the ten species of Fusarium. This is the first report of an alternavirus infecting F. avenaceum and conferring hypervirulence.

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“…The finding of a novel virus in Aspergillus foetidus (AfV-F) that was phylogenetically related to AaV1 led to the proposal of the new family " Alternaviridae " with AaV1 as its type member [ 3 ]. Members of this proposed family possess a genome consisting of three to four monocistronic dsRNA segments, which range from 1.4 kbp (dsRNA 4) to 3.6 kbp (dsRNA 1) [ 3 – 11 ], and a polyA tail at the 3’ end. Wu et al [ 12 ] and Lutz et al [ 10 ] experimentally showed that the 5’ ends of the AaV1 and of Fusarium solani alternavirus 1 (FsAV1) genome segments are capped.…”

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confidence: 99%

Complete genome sequence of a novel alternavirus infecting the fungus Ilyonectria crassa

2023

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A novel dsRNA mycovirus named Ilyonectria crassa alternavirus 1 (IcAV1) was found in Ilyonectria crassa isolate NW-FVA 1829. The fungus was isolated from an ash (Fraxinus excelsior L.) necrotic trunk disc infected with Hymenoscyphus fraxineus [(T. Kowalski) Baral, Queloz, Hosoya] causing ash dieback. The complete genome of IcAV1 is composed of three segments, each containing a single ORF on the positive-sense RNA. The extreme 5’ UTRs of dsRNA 1 (3604 bp), dsRNA 2 (2547 bp), and dsRNA 3 (2518 bp) share a conserved hexadecamer sequence (5’-GGCTGTGTGTTTAGTT-3’) and are capped. The 3’ UTRs are polyadenylated. In silico analysis showed that the viral RdRP is encoded on dsRNA 1 and the capsid-protein subunits are encoded on dsRNA 3. Maximum-likelihood analysis of the aa sequence of the viral RdRP showed that IcAV1 clusters with alternaviruses from Fusarium spp., while the type member of the proposed family "Alternaviridae", Alternaria alternata virus 1 (AaV1), formed a clade together with Stemphylium lycopersici mycovirus (SlV). The function of the protein encoded on segment 2 is unknown. Based on its genome organization and its phylogenetic position, IcAV1 is suggested to be a new member of the proposed family "Alternaviridae". This is the first report of a mycovirus infecting I. crassa.

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Molecular characterization of a novel alternavirus infecting the entomopathogenic fungus Cordyceps chanhua

Cited by 14 publications

References 16 publications

A Novel Gammapartitivirus That Causes Changes in Fungal Development and Multi-Stress Tolerance to Important Medicinal Fungus Cordyceps chanhua

A Novel Gammapartitivirus That Causes Changes in Fungal Development and Multi-Stress Tolerance to Important Medicinal Fungus Cordyceps chanhua

Characterization of the First Alternavirus Identified in Fusarium avenaceum, the Causal Agent of Potato Dry Rot

Complete genome sequence of a novel alternavirus infecting the fungus Ilyonectria crassa

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