2002
DOI: 10.1128/aac.46.3.638-645.2002
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Molecular Characterization of a Novel Class 1 Integron Containing bla GES-1 and a Fused Product of aac(3)-Ib/aac(6 " )-Ib " Gene Cassettes in Pseudomonas aeruginosa

Abstract: As seen by the disk diffusion method, the clinical strain of Pseudomonas aeruginosa Pa695, resistant to all extended-spectrum cephalosporins and aminoglycosides, exhibited an unusual synergistic effect between ceftazidime and imipenem. This isolate produced an extended-spectrum ␤-lactamase (ESBL) with a pI of 5.8 that appeared to be chromosomally encoded. Cloning experiments revealed that this ESBL was encoded by bla GES-1 , previously described in an integron from Klebsiella pneumoniae. In P. aeruginosa Pa695… Show more

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Cited by 130 publications
(96 citation statements)
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“…DNA in supernatant was used as a template under standard PCR procedure [9] with a series of different sets of primers. Detection of the known acquired CHDLs (bla OxA-23, bla , and bla OxA-58 ) [14], class A extended-spectrum b-lactamases (bla PER , bla VEB , and bla GES ) [14,15], MBL (bla IMP , bla VIM , bla SIM , bla NDM ) [16,17] and the intrinsic bla OxA-51-like gene was performed as previously described [14].…”
Section: Pcr Amplification and Plasmid Typingmentioning
confidence: 99%
“…DNA in supernatant was used as a template under standard PCR procedure [9] with a series of different sets of primers. Detection of the known acquired CHDLs (bla OxA-23, bla , and bla OxA-58 ) [14], class A extended-spectrum b-lactamases (bla PER , bla VEB , and bla GES ) [14,15], MBL (bla IMP , bla VIM , bla SIM , bla NDM ) [16,17] and the intrinsic bla OxA-51-like gene was performed as previously described [14].…”
Section: Pcr Amplification and Plasmid Typingmentioning
confidence: 99%
“…Treatment options for established Pseudomonal infections are always a difficult task due to its problematic multidrug resistance traits 6 . Antibiotic resistance characteristics in P. aeruginosa are both chromosomal and acquired or by horizontal transfer of resistance determinant often carried within plasmids and integrons 7 . Prescriptions for antipseudomonal drugs are combination therapy for effective synergy preventing the development of resistance in the course of treatments 1 …”
Section: Introductionmentioning
confidence: 99%
“…Such multiple acetylation has not been documented for any other known AAC. To confirm the uniqueness of multiacetylation by Eis, we tested six other AACs known to perform monoacetylation at the 2′-, 3-, or 6′ positions (8)(9)(10)(11)(12)(13)(14)(15)(16). UV-Visible (UV-Vis) and mass spectrometry assays of these six AACs with the ten AGs that are multiacetylated by Eis, showed only monoacetylation.…”
mentioning
confidence: 99%