Molecular Characterization of Akebia Plants and the Derived Traditional Herbal Medicine

Kitaoka, Fumiyo; Kakiuchi, Nobuko; Chen, Long; Itoga, Mai; Mitsue, Akihiro; Mouri, Chika; Mikage, Masayuki

doi:10.1248/bpb.32.665

Cited by 21 publications

(12 citation statements)

References 11 publications

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“…3,4) In the past decades, a number of studies have shown that several natural TCM products can activate cell death pathway in cancer cells including leukemia.…”

mentioning

confidence: 99%

20(<i>S</i>)-Ginsenoside Rh2 Induces Apoptosis in Human Leukaemia Reh Cells through Mitochondrial Signaling Pathways

Xia

Wang

et al. 2014

Biological & Pharmaceutical Bulletin

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“…3,4) In the past decades, a number of studies have shown that several natural TCM products can activate cell death pathway in cancer cells including leukemia.…”

mentioning

confidence: 99%

20(<i>S</i>)-Ginsenoside Rh2 Induces Apoptosis in Human Leukaemia Reh Cells through Mitochondrial Signaling Pathways

Xia

Wang

et al. 2014

Biological & Pharmaceutical Bulletin

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“…The herb was used as an important crude drug for promoting urination and counteracting inflammation in traditional Chinese medicine. 1 Akebia quinata, which is also known as the chocolate vine, is a woody perennial plant that grows either as a twining vine or as groundcover. The plant is distributed widely in East Asia, including: China, Japan and Korea.…”

Section: Introductionmentioning

confidence: 99%

“…In 2009, Fumiyo Kikaota et al 1 performed a molecular biological identification of Akebia plant species and discriminating Akebiae Caulis from nonAkebia plants. In a report on the characterization of the stems of Akebia trifoliata, Yoshihiro Mimaki et al 3 identified four saponins that were obtained in good yield but were not isolated from A. quinata stems.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Determination of Compounds from Akebia quinata by High-Performance Liquid Chromatography

Yến¹,

Thụ²,

Zhao³

et al. 2014

Bulletin of the Korean Chemical Society

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To provide the scientific corroboration of the traditional uses of Akebia quinata (Thunb.) Decne., a detailed analytical examination of A. quinata stems was carried out using a reversed-phase high performance liquid chromatography (RP-HPLC) method coupled to photodiode array detector (PDA) for the simultaneous determination of four phenolic substances; cuneataside D (1), 2-(3,4-dihydroxyphenyl)ethyl-O-β-D-glucopyranoside (2), 3-caffeoylquinic acid (3) and calceolarioside B (4). Particular attention was focused on the main compound, 3-caffeoylquinic acid (3), which has a range of biological functions. In addition, 2-(3,4-dihydroxyphenyl)ethyl-O-β-D-glucopyranoside (2) was considered as a discernible marker of A. quinata from its easy confuse plants. The contents of compounds 2 and 3 ranged from 0.72 to 2.68 mg/g and from 1.66 to 5.64 mg/g, respectively. The validation data indicated that this HPLC/PDA assay was used successfully to quantify the four phenolic compounds in A. quinata from different locations using relatively simple conditions and procedures. The pattern-recognition analysis data from 53 samples classified them into two groups, allowing discrimination between A. quinata and comparable herbs. The results suggest that the established HPLC/PDA method is suitable for quantitation and pattern-recognition analyses for a quality evaluation of this medicinal herb.

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“…With the advancement of molecular technology, DNA markers have now become a convenient means for species identification and molecular systematic study [9-11] and many DNA markers have in fact been patented for further development [12]. With the help of polymerase chain reaction (PCR), specific DNA regions can be amplified from only a small amount of samples.…”

Section: Introductionmentioning

confidence: 99%

An integrated web medicinal materials DNA database: MMDBD (Medicinal Materials DNA Barcode Database)

Lou

Wong

et al. 2010

BMC Genomics

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BackgroundThousands of plants and animals possess pharmacological properties and there is an increased interest in using these materials for therapy and health maintenance. Efficacies of the application is critically dependent on the use of genuine materials. For time to time, life-threatening poisoning is found because toxic adulterant or substitute is administered. DNA barcoding provides a definitive means of authentication and for conducting molecular systematics studies. Owing to the reduced cost in DNA authentication, the volume of the DNA barcodes produced for medicinal materials is on the rise and necessitates the development of an integrated DNA database.DescriptionWe have developed an integrated DNA barcode multimedia information platform- Medicinal Materials DNA Barcode Database (MMDBD) for data retrieval and similarity search. MMDBD contains over 1000 species of medicinal materials listed in the Chinese Pharmacopoeia and American Herbal Pharmacopoeia. MMDBD also contains useful information of the medicinal material, including resources, adulterant information, medical parts, photographs, primers used for obtaining the barcodes and key references. MMDBD can be accessed at http://www.cuhk.edu.hk/icm/mmdbd.htm.ConclusionsThis work provides a centralized medicinal materials DNA barcode database and bioinformatics tools for data storage, analysis and exchange for promoting the identification of medicinal materials. MMDBD has the largest collection of DNA barcodes of medicinal materials and is a useful resource for researchers in conservation, systematic study, forensic and herbal industry.

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Molecular Characterization of Akebia Plants and the Derived Traditional Herbal Medicine

Cited by 21 publications

References 11 publications

20(<i>S</i>)-Ginsenoside Rh2 Induces Apoptosis in Human Leukaemia Reh Cells through Mitochondrial Signaling Pathways

20(<i>S</i>)-Ginsenoside Rh2 Induces Apoptosis in Human Leukaemia Reh Cells through Mitochondrial Signaling Pathways

Quantitative Determination of Compounds from Akebia quinata by High-Performance Liquid Chromatography

An integrated web medicinal materials DNA database: MMDBD (Medicinal Materials DNA Barcode Database)

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