Molecular characterization of an ecdysteroid inducible carboxylesterase with GQSCG motif in the corn borer, Sesamia nonagrioides

Kontogiannatos, Dimitriοs; Michail, Xenia; Kourti, Anna

doi:10.1016/j.jinsphys.2011.04.017

Cited by 25 publications

(26 citation statements)

References 42 publications

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“…Insect rearing and determining the stage of development of the larvae S. nonagrioides insects were maintained at 25°C, 55 ± 5% relative humidity on an artifi cial diet (Kontogiannatos et al, 2011(Kontogiannatos et al, , 2013. Larvae reared under 16L : 8D conditions completed their larval stage in 6 instars.…”

Section: Methodsmentioning

confidence: 99%

“…Closely related enzymes, the juvenile hormone esterase related enzymes (JHERs), contain a cysteine residue immediately adjacent to the catalytic serine, in contrast to most other described esterases, including JHE, which have alanine at this position (Jones et al, 1994;Kontogiannatos et al, 2011). In Trichoplusia ni it is proposed that a JH-like compound could be the target of the JHER enzyme (Jones et al, 1994).…”

Section: Plasmids and Virus Constructsmentioning

confidence: 99%

“…In S.nonagrioides we characterized a JHER gene that has GQSCG instead of the normal GQSAG catalytic motif on its predicted protein (SnJHER) (Kontogiannatos et al, 2011). This gene is not responsive to the juvenile hormone analog (JHA) methoprene, but it is positively regulated by ecdysteroid analogs and the xenobiotic bisphenol A (BPA) (Kontogiannatos et al, 2011).…”

Section: Plasmids and Virus Constructsmentioning

confidence: 99%

“…This gene is not responsive to the juvenile hormone analog (JHA) methoprene, but it is positively regulated by ecdysteroid analogs and the xenobiotic bisphenol A (BPA) (Kontogiannatos et al, 2011). Depletion of SnJHER by RNAi revealed its potential role in the regulation of the developmental programming in S. nonagrioides.…”

Section: Plasmids and Virus Constructsmentioning

confidence: 99%

“…Fifth instar larvae on day 1 (low SnJHERs expression; Kontogiannatos et al, 2011Kontogiannatos et al, , 2016 were injected with 50 μl of 10 7 pfu/ml of the BmNPV-BmA::GFP/P10::NFlag/SnJHER virus or control BmNPV/BmA::GFPBmA::dsLuciferase virus. There were three replicates of this experiment: n1 = 32, n2 = 33, n3 = 35 insects each (total n = 100).…”

Section: Cytotoxicity Of Jhas In Jher Expressing Cell Linesmentioning

confidence: 99%

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Abnormal development in larvae of Sesamia nonagrioides (Lepidoptera: Noctuidae) resulting from baculovirus-mediated overexpression of a JHE-related gene (SnJHER)

Kontogiannatos¹,

Swevers²,

Kourti³

2017

Eur. J. Entomol.

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Abstract. The Mediterranean corn borer Sesamia nonagrioides (Lepidoptera: Noctuidae) has a unique and recently multiplied juvenile hormone esterase gene family (SnJHER) with particular transcriptional profi les and functional characteristics. Unlike conventional juvenile hormone esterase genes (JHEs), the SnJHER gene family seems to have been recently evolved from a common ancestral JHER gene. SnJHERs seem to be regulated by both ecdysone agonists and xenobiotics, while their real role in development remains to be exploited. In this study we transiently expressed the major SnJHER isoform in Bm5 and Hi5 cell lines. The JHER-expressing cell lines showed increased toxicity when treated with the juvenile hormone analog methoprene. Moreover baculovirus-mediated transient gene transduction of the SnJHER gene in larvae of S. nonagrioides resulted in moulting abnormalities. These were more marked after the additional application of the juvenile hormone analog methoprene. Our results indicate a potential mechanism by which SnJHER interferes with normal JHE.

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Section: Methodsmentioning

confidence: 99%

Section: Plasmids and Virus Constructsmentioning

confidence: 99%

Section: Plasmids and Virus Constructsmentioning

confidence: 99%

Section: Plasmids and Virus Constructsmentioning

confidence: 99%

Section: Cytotoxicity Of Jhas In Jher Expressing Cell Linesmentioning

confidence: 99%

See 3 more Smart Citations

Abnormal development in larvae of Sesamia nonagrioides (Lepidoptera: Noctuidae) resulting from baculovirus-mediated overexpression of a JHE-related gene (SnJHER)

Kontogiannatos¹,

Swevers²,

Kourti³

2017

Eur. J. Entomol.

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The expression patterns of the clock genes period and timeless are affected by photoperiod in the Mediterranean corn stalk borer, Sesamia nonagrioides

Kontogiannatos

Gkouvitsas

Kourti

2016

Arch Insect Biochem Physiol

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To obtain clues to the link between the molecular mechanism of circadian and photoperiod clocks, we cloned two circadian clock genes, period (per) and timeless (tim) from the moth Sesamia nonagrioides, which undergoes facultative diapause controlled by photoperiod. Sequence analysis revealed a high degree of conservation among the compared insects fοr both genes. We also investigated the expression patterns of per and tim in brains of larvae growing under 16L:8D (long days), constant darkness (DD) and 10L:14D (short days) conditions by qPCR assays. The results showed that mRNA accumulations encoding both genes exhibited diel oscillations under different photoperiods. The oscillation of per and tim mRNA, under short-day photoperiod differed from long-day. The difference between long-day and short-day conditions in the pattern of mRNA levels of per and tim appears to distinguish photoperiodic conditions clearly and both genes were influenced by photoperiod in different ways. We infer that not all photoperiodic clocks of insects interact with circadian clocks in the same fashion. Our results suggest that transcriptional regulations of the both clock genes act in the diapause programing in S. nonagrioides. The expression patterns of these genes are affected by photoperiod but runs with 24 h by entrainment to daily environmental cues.

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Isolation of CarE genes from the Chinese white pine beetle Dendroctonus armandi (Curculionidae: Scolytinae) and their response to host chemical defense

Dai

Gao

et al. 2018

Pest Management Science

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Molecular characterization of an ecdysteroid inducible carboxylesterase with GQSCG motif in the corn borer, Sesamia nonagrioides

Cited by 25 publications

References 42 publications

Abnormal development in larvae of Sesamia nonagrioides (Lepidoptera: Noctuidae) resulting from baculovirus-mediated overexpression of a JHE-related gene (SnJHER)

Abnormal development in larvae of Sesamia nonagrioides (Lepidoptera: Noctuidae) resulting from baculovirus-mediated overexpression of a JHE-related gene (SnJHER)

The expression patterns of the clock genes period and timeless are affected by photoperiod in the Mediterranean corn stalk borer, Sesamia nonagrioides

Isolation of CarE genes from the Chinese white pine beetle Dendroctonus armandi (Curculionidae: Scolytinae) and their response to host chemical defense

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