Molecular characterization of an endophytic Phomopsisliquidambaris CBR-15 from Cryptolepis buchanani Roem. and impact of culture media on biosynthesis of antimicrobial metabolites

Rao, H.C. Yashavantha; Parthasarathy, S.; Rakshith, Devaraju; Satish, S.

doi:10.1007/s13205-014-0204-2

Cited by 16 publications

(7 citation statements)

References 37 publications

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“…Forest trees confer the largest niche for endophytic fungal diversity[26]. In fact, it is known that endophytic fungi isolated from plants show antimicrobial and antioxidant activities [18, 20, 22], including other bioactivities such as enzyme activity [27], volatile biocontrol agents [28] and volatile compounds having fuel potentials [9, 29, 30]. Moreover, endophytic fungi have the ability to produce similar or unique bioactive secondary metabolites compared to host plant metabolites [4, 5, 26, 31].…”

Section: Discussionmentioning

confidence: 99%

“…To test the production of intracellular and secreted extracellular secondary metabolites, a small-scale fermentation process was carried out as previously described [18]. Briefly, fungi were grown in 250 ml Erlenmeyer flasks containing 100 ml of PDB at 25 ±2 °C and shaken at 150 rpm.…”

Section: Methodsmentioning

confidence: 99%

“…Altogether, six human pathogenic-bacteria and one human yeast-pathogen were used in an agar-well diffusion (AWD) assay, to determine the antimicrobial activity of the crude extracts obtained from the fungal endophytes [11, 18, 20]. Stocks (10 mg/ml) for both the intracellular and extracellular-extracts were made using DMSO as a solvent.…”

Section: Methodsmentioning

confidence: 99%

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Annulohypoxylonsp. strainMUS1, an Endophyte isolated fromTaxus wallichianaZucc. produces Taxol and Other Bioactive Metabolites

Gauchan

Vélëz

Acharya

et al. 2020

Preprint

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24Endophytes are microbial colonizers that reside in plants by symbiotic association produces 25 several biological classes of natural products. The current study focuses on the isolation and 26 characterization of bioactive compounds produced by endophytic fungi isolated from the 27 Himalayan yew (Taxus wallichiana) collected from the Mustang district of Nepal. The plant 28 materials were collected from the Lower-Mustang region in the month of October 2016 and the 29 endophytic fungi were isolated on artificial media from inner tissues of bark and needles. 30 Antimicrobial and antioxidant activity, along with total phenolic-and flavonoid-content assays, 31 were used in the evaluation of bioactivity of the fermented crude extracts along with the in vitro 32 ability of the endophytes to produce the anticancer compound Taxol was analyzed. A total of 16 33 fungal morphotypes were obtained from asymptomatic inner tissues of the bark and needles of T. 34 wallichiana. Among the 16 isolates, the ethyl acetate (EA) fraction of isolate MUS1, showed 35 antibacterial and antifungal activity against all test-pathogens used, with significant inhibition 36 against Pseudomonas aeruginosa ATCC 27853 (MIC: 250 µg/ml) and the pathogenic yeast, 37 Candida albicans (MIC: 125 µg/ml). Antioxidant activity was also evaluated using 2,2-diphenyl-38 1-picrylhydrazyl (DPPH). At a concentration of 100 µg/ml, the % radical scavenging activity 39 was 83.15±0.40, 81.62±0.11, and 62.36±0.29, for ascorbic acid, butylated hydroxytoluene (BHT) 40 and the EA fraction of MUS1, respectively. The DPPH-IC50 value for the EA fraction was 81.52 41 µg/ml, compared to BHT (62.87 µg/ml) and ascorbic acid (56.15 µg/ml). The total phenolic and 42 flavonoid content in the EA fraction were 16.90±0.075 µg gallic acid equivalent (GAE) and 43 11.59±0.148 µg rutin equivalent (RE), per mg of dry crude extract, respectively. Isolate MUS1, 44 identified as an Annulohypoxylon sp. by ITS sequencing, also produced Taxol (282.05 µg/L) as 45 shown by TLC and HPLC analysis. Having the ability to produce antimicrobial and antioxidant 46 3 compounds, as well as the anticancer compound Taxol, makes Annulohypoxylon sp. strain 47 MUS1, a promising candidate for further study given that naturally occurring bioactive 48 compounds are of great interest to the pharmacological, food and cosmetic industries.49

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Annulohypoxylonsp. strainMUS1, an Endophyte isolated fromTaxus wallichianaZucc. produces Taxol and Other Bioactive Metabolites

Gauchan

Vélëz

Acharya

et al. 2020

Preprint

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“…Endophytes are of the highly merited microbial sources owing to its adaptation to unique biological niches in higher plants. Studies also confer that these endophytes are capable of secreting structurally diverse classes of secondary metabolites bearing activities [ 30 ]. In present investigation, bacterial endophytes inhabiting Euphorbia hirta L. were screened for synthesis of silver nanoparticles.…”

Section: Discussionmentioning

confidence: 99%

Biomimetic Synthesis of Silver Nanoparticles Using Endosymbiotic Bacterium InhabitingEuphorbia hirtaL. and Their Bactericidal Potential

et al. 2016

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The present investigation aims to evaluate biomimetic synthesis of silver nanoparticles using endophytic bacterium EH 419 inhabiting Euphorbia hirta L. The synthesized nanoparticles were initially confirmed with change in color from the reaction mixture to brown indicating the synthesis of nanoparticles. Further confirmation was achieved with the characteristic absorption peak at 440 nm using UV-Visible spectroscopy. The synthesized silver nanoparticles were subjected to biophysical characterization using hyphenated techniques. The possible role of biomolecules in mediating the synthesis was depicted with FTIR analysis. Further crystalline nature of synthesized nanoparticles was confirmed using X-ray diffraction (XRD) with prominent diffraction peaks at 2θ which can be indexed to the (111), (200), (220), and (311) reflections of face centered cubic structure (fcc) of metallic silver. Transmission electron microscopy (TEM) revealed morphological characteristics of synthesized silver nanoparticles to be polydisperse in nature with size ranging from 10 to 60 nm and different morphological characteristics such as spherical, oval, hexagonal, and cubic shapes. Further silver nanoparticles exhibited bactericidal activity against panel of significant pathogenic bacteria among which Pseudomonas aeruginosa was most sensitive compared to other pathogens. To the best of our knowledge, present study forms first report of bacterial endophyte inhabiting Euphorbia hirta L. in mediating synthesizing silver nanoparticles.

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“…Similarly, CB is a climber or straggling shrub commonly found in forests of Thailand, China, India, Nepal, and Indo-china [18]. Different parts of this plant used for anti-diarrheal [19], anti-bacterial [20], anti-ulcerative [21], anti-inflammatory [22], rickets [23], anti-diarrheal [24], and blood purifier [25] due to the presence of alkaloids, steroids, glycosides, saponins, flavonoids and tannins, calcium, and phosphates [26]. Sardinella Longiceps (SL) is a species of ray-finned fish found in Indian, Pacific, and Atlantic oceans [27].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of bone healing activity of Cissus quadrangularis (Linn), Cryptolepis buchanani, and Sardinella longiceps in Wistar rats

Ramachandran¹,

Fadhil²,

Gopi³

et al. 2021

Beni-Suef Univ J Basic Appl Sci

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Background The object of the present study is to evaluate the effect of alcoholic extracts of Cissus quadrangularis (CQ), Cryptolepis buchanani (CB), and Sardinella longiceps (SL) either alone or in the combination (100 mg/kg) in the management of femur bone healing of Wistar albino rats for 8 weeks. After the period of treatment, femur bones were examined by using biochemical, radiographical, and histopathological studies. Result Biochemical evaluation results reveal that there is a steep increase of serum calcium level in the experimental animals during the entire period of treatment which led to an adequate supply of serum calcium to the fractured bone for healing and increases the thickness of the femur bones soon compared to control group. It had been estimated by a calibrated ocular micrometer. Radiographical images of the bones also disclose that the complete bridging of fractured bone occurred in the experimental animals after the treatment of natural compound extracts. In addition to that, all the organs of animals were safe in the experimental animals during the entire study. Conclusion The present study strongly recommended that these ethanolic extracts (CQCBSL) either alone or in the combination restoring the strength of the bone and reduced bone repairing period due to the rich content of calcium and other natural phytochemicals presents with them. Graphical abstract

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Molecular characterization of an endophytic Phomopsisliquidambaris CBR-15 from Cryptolepis buchanani Roem. and impact of culture media on biosynthesis of antimicrobial metabolites

Cited by 16 publications

References 37 publications

Annulohypoxylonsp. strainMUS1, an Endophyte isolated fromTaxus wallichianaZucc. produces Taxol and Other Bioactive Metabolites

Annulohypoxylonsp. strainMUS1, an Endophyte isolated fromTaxus wallichianaZucc. produces Taxol and Other Bioactive Metabolites

Biomimetic Synthesis of Silver Nanoparticles Using Endosymbiotic Bacterium InhabitingEuphorbia hirtaL. and Their Bactericidal Potential

Evaluation of bone healing activity of Cissus quadrangularis (Linn), Cryptolepis buchanani, and Sardinella longiceps in Wistar rats

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