Molecular Characterization of Barberry Genotypes from Turkey and Kyrgyzstan

Pınar, Hasan; Yahya, Haider N.; Erċışlı, Sezai; Coşkun, Ömer Faruk; Yaman, Mehmet; Turgunbaev, Kubanichbek; Uzun, Aydın

doi:10.1007/s10341-021-00599-x

Cited by 18 publications

(5 citation statements)

References 14 publications

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“…This specificity reduces the likelihood of nonspecific amplification, resulting in more consistent and reliable results across experiments. Additionally, iPBS primers typically generate fewer artifacts and background noise, further enhancing their reproducibility compared to AFLP [33,[37][38][39][40]. Even though the accessions gathered from Sahiwal and Faisalabad exhibited a level of polymorphism similar to that observed in previous studies, this suggests a notable degree of polymorphism within the accessions examined in the current study.…”

Section: Discussionsupporting

confidence: 69%

Assessment of Molecular Diversity and Population Structure of Pakistani Mulberry Accessions Using Retrotransposon-Based DNA Markers

Mehmood,

Dracatos,

Arshad

et al. 2024

Agriculture

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Both morphological and molecular markers have been extensively used to evaluate genetic diversity; however, molecular markers are considered more reliable and can lead to improved reproductive efficiency. This study utilized inter-primer binding site (iPBS) markers to examine the genetic diversity and population structure of thirty mulberry accessions from the districts of Sahiwal and Faisalabad, Pakistan. These mulberry accessions belonged to three species: Morus nigra (n = 13), Morus alba (n = 12), and Morus rubra (n = 5). The use of nine iPBS primers in this study provided a comprehensive understanding of genetic diversity among the selected mulberry accessions. Nine iPBS primers were used in the study and generated 431 bands with allelic frequencies ranging from 21 to 75 and band sizes from 200 to 1500 base pairs. The primer 2230 showed the highest polymorphic information content (PIC) value of 0.47 and the highest Shannon’s information index (I = 0.53). The Morus nigra accessions had the highest levels of expected heterozygosity (He = 0.30), unbiased expected heterozygosity (µHe = 0.33), and Shannon’s information index (I = 0.45). The molecular variance analysis (AMOVA) revealed a high degree of genetic variation, as estimated by the pairwise PhiPT value of 0.21, which was significant at the p < 0.001 *** level. The neighbor joining tree, principal coordinate analysis, and structure analysis grouped the 30 mulberry accessions into four main clusters. The distinct grouping of accessions SWLS14, SWLS6, FSDS30, and SWLS7 validated their notable genetic distinctiveness. Overall, these findings contribute valuable insights into the genetic landscape of mulberry accessions, which are essential for conservation and breeding strategies.

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Section: Discussionsupporting

confidence: 69%

Assessment of Molecular Diversity and Population Structure of Pakistani Mulberry Accessions Using Retrotransposon-Based DNA Markers

Mehmood,

Dracatos,

Arshad

et al. 2024

Agriculture

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“…Marker-assisted selection can be achieved by characterizing germplasm that allows the breeders to identify new genetic variations and use them to select parent lines for further crossing [9]. To date, a large number of genetic markers have been developed to identify genetic variations in various crop species [10][11][12]. Among the markers, retrotransposons are mobile elements that have the ability to change their position, duplicate themselves, and play a key role in plant genome size, and can be used as molecular markers [13].…”

Section: Introductionmentioning

confidence: 99%

Exploring the Agronomic Performance and Molecular Characterization of Diverse Spring Durum Wheat Germplasm in Kazakhstan

Tajibayev,

Mukin,

Babkenov

et al. 2023

Agronomy

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Spring durum wheat occupies over 0.5 M ha in Kazakhstan and represents an important domestic and export commodity. This study aimed to characterize 151 durum wheat cultivars and advanced lines originating from eight breeding programs of the Kazakhstan–Siberia Spring Wheat Improvement Network (KASIB) between 2003 and 2018. The phenotypic characterization was performed in two contracting evaluation sites more than 1000 km apart (Almaty in the Southeast and Shortandy in the North) for two years and a total of 11 agronomic traits were recorded. Field trials at both locations followed regional agronomy practices, including sowing, harvesting, and genotype evaluation using a randomized complete block design (RCBD). The growing season was longer in Almaty, resulting in a higher number of grains per spike. Though grains are smaller in size with an overall higher yield, 243 g/m2 versus 170 g/m2, there was no correlation between germplasm performance at the two sites. Molecular characterization was performed with 10 iPBS-retrotransposons primers that resulted in a total of 345 bands and showed a mean polymorphism of 91.9%. Mean values of gene diversity (0.251), Shannon’s information index (0.388), and expected heterozygosity (0.233) revealed a relatively high level of genetic diversity in the KASIB set. AMOVA revealed higher genetic variations due to differences within the populations. Marker-based cluster analysis, including STRUCTURE and neighbor-joining algorithms, divided the material into two populations with clear differences in geographic origin. Superiors and diverse germplasm identified in the study are recommended for marker assisted selection and breeding.

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“…These herbicides have a broader spectrum of action, covering perennial, single and dicotyledonous weeds as well as cereals. This is due to market needs, where high cultivation intensity of sunflower hybrids and reduced crop care costs play an important role in reducing the cost of final production (Pinar et al, 2021). Researchers A.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of sunflower breeding material for resistance to broomrape (Orobanche cumana Wallr.) and herbicides under artificial climate conditions to accelerate the breeding process

Shcherban,

Abitayev

et al. 2023

Scientific Horizons

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Sunflower is an important oilseed crop, so increasing its yield and resistance to diseases and pests can lead to significant economic improvements for agricultural businesses and the country as a whole. Climate change and the spread of new races of broomrape (Orobanche cumana Wallr.) pose serious threats to sunflower production, and research in this area is helping to develop varieties that can adapt to changing conditions. The purpose of the study is a comprehensive assessment of breeding material to identify the most resistant forms and hybrids of sunflower. To achieve the goal, an experiment was conducted in the period 2015-2023 in the LLP “Experimental Farm of Oilseed Crops”, in which the assessment of sunflower breeding material for resistance to broomrape and herbicides was studied. As a result of the experiment, sunflower lines were successfully created that are resistant to broomrape and herbicides, which helps to increase productivity and reduce crop losses. The created sunflower lines were introduced into the breeding process in order to develop highly productive interline sunflower hybrids, which became an important step in improving the agriculture of Kazakhstan. These innovative hybrids, such as “Agribusiness 2050” and “Batyr”, have shown high resistance to broomrape of races E-F, which significantly reduces crop losses and ensures reliable products. In addition, herbicide-resistant hybrids have been developed, such as Baiterek S and Baikonur, which can effectively control weeds and maintain crop cleanliness, which is important for increasing yields and reducing tillage costs. These hybrids have been recommended for use in agriculture in Kazakhstan and can help improve sunflower productivity. This study contributes to the expansion of scientific knowledge in the field of sunflower breeding and pest control, which is a relevant area for improving agricultural practices and ensuring food security

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Molecular Characterization of Barberry Genotypes from Turkey and Kyrgyzstan

Cited by 18 publications

References 14 publications

Assessment of Molecular Diversity and Population Structure of Pakistani Mulberry Accessions Using Retrotransposon-Based DNA Markers

Assessment of Molecular Diversity and Population Structure of Pakistani Mulberry Accessions Using Retrotransposon-Based DNA Markers

Exploring the Agronomic Performance and Molecular Characterization of Diverse Spring Durum Wheat Germplasm in Kazakhstan

Evaluation of sunflower breeding material for resistance to broomrape (Orobanche cumana Wallr.) and herbicides under artificial climate conditions to accelerate the breeding process

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