Molecular Characterization of blaCTX-M, blaTEM and blaSHV Beta Lactamases Produced by Uropathogens

Bhasin, Ashna; Chander, Yogesh; Manocha, Harmesh

doi:10.9734/jammr/2020/v32i2330721

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…The bacteria that produce extended spectrum βlactamase (ESBL) hydrolyze oxyimino β-lactams and monobactams, and even though have no impact on cephamycins and carbapenems are known as ESBL generating strains. ESBL forming pathogens have become increasingly resistant to antibiotics, such as clavulanic acid, sulbactam, and tazobactam, as a result of the evolution of ESBL-forming organisms (19). The prevalence and dispersion of ESBLs varies from one geographical area to another, as well as from one hospital to the next.…”

Section: Susceptibility Testingmentioning

confidence: 99%

Molecular detection of β-lactamase genes in Klebsiella pneumoniae and Escherichia coli isolated from different clinical sources

Bakr

Abdul-Rahman

Hamasalih

2022

Cell Mol Biol (Noisy-le-grand)

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The rising occurrence of infections generated by Escherichia coli and Klebsiella pneumoniae that produce extended-spectrum β-lactamase (ESBL) is reason for concern. Due to the recent emergence of multidrug-resistant microorganisms that develop ESBL. The purpose of this work was to detect the ESBLs in clinical isolates of E. coli and K. pneumoniae. 118 samples of E. coli and 63 isolates of K. pneumoniae were collected from clinical samples. Polymerase chain reaction was used to detect β-lactamase genes (i.e., blaTEM, blaSHV, and blaCTX-M). Phenotypic detection revealed that 48.31% and 85.19% of E. coli and K. pneumoniae produced ESBLs, respectively. Whereas screening of ESBL genes in both bacteria employing a multiplex PCR test revealed that 24.58% of the ESBL-producing E. coli strains contained blaTEM, 50.85% contained blaSHV, and 32.2% contained blaCTX-M. Nevertheless, in K. pneumoniae, 40.74% blaTEM, 35.19% blaSHV, and 64.81% blaCTX-M genes were present. Antimicrobial resistance profiles of E. coli and K. pneumoniae isolates to twenty antibiotics were observed to vary significantly. Additionally, it was determined that the majority of E. coli and K. pneumoniae isolates were multidrug resistant (MDR). Additionally, 80.51% of E. coli isolates were resistant to the AMC antibiotic, while 0.00% were resistant to IPM and MEM. From the other hand, the resistant proportion of K. pneumoniae isolates was heterogeneous, ranging from 69.84% against CAZ to 0.00% against CIP and G antibiotics. The blaSHV gene was the most widespread among different forms of ESBLs in E. coli, but the most common gene in K. pneumoniae isolates was blaCTX-M (64.81%).

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Section: Susceptibility Testingmentioning

confidence: 99%

Molecular detection of β-lactamase genes in Klebsiella pneumoniae and Escherichia coli isolated from different clinical sources

Bakr

Abdul-Rahman

Hamasalih

2022

Cell Mol Biol (Noisy-le-grand)

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“…The primers for amplification Table (1) were procured from Imperial Life Sciences Ltd. (Gurgaon, India) (11) and PCR was conducted in BIO-RAD CFX Connect thermocycler.…”

Section: Genotypic Confirmation Of Esbl Productionmentioning

confidence: 99%

Prevalence of Extended Spectrum Beta‑Lactamase in Pseudomonas aeruginosa isolated from patients at Sharda Hospital, Greater Noida, Western UP

et al. 2022

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ABSTRACT Introduction: Pseudomonas aeruginosa is an important pathogen causing healthcare-associated infections especially in immunocompromised patients. It poses a threat to public health due to its inherent resistance to various antimicrobial agents and its ability to acquire new resistance through multiple mechanisms. Infections due to extended spectrum β-lactamase (ESBL) producing isolates of P. aeruginosa continue to be a challenge for clinicians as these result in high mortality and morbidity due to antimicrobial resistance. The aim of this study was to determine the prevalence of Cefotaximase-Munich (CTX-M) producing strains of P. aeruginosa in Sharda hospital, Greater Noida. Methods: Strains of P. aeruginosa isolated from various clinical samples were subjected to phenotypic detection for ESBL production by disc combination method. Positive strains were then subjected to polymerase chain reaction (PCR) for detection of blaCTX-M gene. Results: Out of 166 isolates of P. aeruginosa, 54 (32.53%) were phenotypically confirmed to produce ESBL. Out of these 54 isolates, 39 (72.22%) were positive for blaCTX-M gene. Multidrug resistance was found in 70 (42.17%) isolates. Imipenem was the most effective drug with a sensitivity of 64.86% whereas aztreonam was found to be least effective with sensitivity of only 36.74%. Conclusion: Current study highlights the phenotypic and molecular characterization of CTX-M gene in P.aeruginosa in our hospital set-up. With judicious use of antimicrobials and strict infection control practices, it might be possible to limit the effect of these drug destroying enzymes. Keywords: CTX-M gene, Extended spectrum beta lactamases, Hospital acquired infections, multidrug resistance, Pseudomonas aeruginosa.

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“…The ESBL enzymes confer resistance to an extended range of beta-lactam antibiotics, including the first, third and some fourth generation cephalosporins but not the cephamycins or carbapenems [7,8]. CTX-M is the predominant enzyme type associated with human clinical ESBL-producing E. coli [9][10][11]. There are more than 250 bla CTX-M gene variants, with bla CTX-M-15 being the most common amongst human clinical E. coli [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Genomic epidemiology of ESBL-producingEscherichia colifrom humans and an Aotearoa New Zealand river

Gray,

Biggs,

Midwinter

et al. 2024

Preprint

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In Aotearoa New Zealand, urinary tract infections in humans are commonly caused by extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli. This group of antimicrobial resistant bacteria are often multidrug resistant. However, there is limited information on ESBL-producing E. coli found in the environment and their link with human clinical isolates. In this study, we examined the genetic relationship of environmental and human clinical ESBL-producing E. coli and isolates collected in parallel within the same area over 14 months. Environmental samples were collected from treated effluent, stormwater and multiple locations along an Aotearoa New Zealand river. Treated effluent, stormwater and river water sourced downstream of the treated outflow point were the main sources of ESBL-producing E. coli (7/14 samples, 50.0%; 3/6 samples, 50%; and 15/28 samples, 54% respectively). Whole genome sequence comparison was carried out on 307 human clinical and 45 environmental ESBL-producing E. coli isolates. Sequence type 131 was dominant for both clinical (147/307, 47.9%) and environmental isolates (11/45, 24.4%). The most prevalent ESBL genes were both blaCTX-M-27 and blaCTX-M-15 for the clinical isolates (134/307, 43.6%) and blaCTX-M-15 for the environmental isolates (28/45, 62.2%). A core single nucleotide polymorphism analysis of these isolates suggested that some strains were shared between humans and the local river. These results highlight the importance of understanding different transmission pathways for the spread of ESBL-producing E. coli.

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Molecular Characterization of blaCTX-M, blaTEM and blaSHV Beta Lactamases Produced by Uropathogens

Cited by 4 publications

References 12 publications

Molecular detection of β-lactamase genes in Klebsiella pneumoniae and Escherichia coli isolated from different clinical sources

Molecular detection of β-lactamase genes in Klebsiella pneumoniae and Escherichia coli isolated from different clinical sources

Prevalence of Extended Spectrum Beta‑Lactamase in Pseudomonas aeruginosa isolated from patients at Sharda Hospital, Greater Noida, Western UP

Genomic epidemiology of ESBL-producingEscherichia colifrom humans and an Aotearoa New Zealand river

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