2011
DOI: 10.4238/2011.november.25.1
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Molecular characterization of cajá, Spondias mombin (Anacardiaceae), by RAPD markers

Abstract: ABSTRACT. The arboreal species Spondias mombin L. (Anacardiaceae) is widely distributed in Brazil, where the fruits, known by the common name of cajá, are an important commercial commodity. We evaluated genetic variability among 32 cajá accessions of the Germplasm Collection of Embrapa Meio-Norte using RAPD technique. Reaction conditions for efficient RAPD amplifications were optimized in preliminary tests, and primers were selected from a set designed by the University of British Columbia on the basis of high… Show more

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Cited by 19 publications
(13 citation statements)
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“…The DNA band number amplified by RAMP-PCR was nearly double than that by traditional RAPD-PCR, and also the amplifications were reproducible. Previous studies indicated that the number of bands produced by traditional RAPD amplifies an average of 5 bands per reaction [29]. Our RAMP-PCR amplified DNA band numbers from different Angelica samples were varied from 8 to 14,…”
Section: Discussionmentioning
confidence: 99%
“…The DNA band number amplified by RAMP-PCR was nearly double than that by traditional RAPD-PCR, and also the amplifications were reproducible. Previous studies indicated that the number of bands produced by traditional RAPD amplifies an average of 5 bands per reaction [29]. Our RAMP-PCR amplified DNA band numbers from different Angelica samples were varied from 8 to 14,…”
Section: Discussionmentioning
confidence: 99%
“…É necessário manejar os recursos genéticos de maneira a preservar a máxima variabilidade genética dentro das espécies, porém, sem dados sobre a distribuição dessa variabilidade dentro ou entre populações, decisões importantes sobre o manejo e conservação desses recursos não podem ser tomadas. Estudos de diversidade e estrutura genética em espécies arbustivas e arbóreas são frequentemente relatados na literatura (SANTOS et al, 2007;LIMA et al, 2011), reforçando a necessidade desse tipo de estudo para S. reticulata.…”
Section: Introductionunclassified
“…De acordo com Lima et al (2011), os conhecimentos sobre a variabilidade genética das espécies são um pré-requisito essencial para sua preservação e para o sucesso de programas de melhoramento. Considerando-se a espécie Spondias mombin L., a alta variabilidade genéti-ca entre os acessos tem sido confirmada por inúmeros estudos, envolvendo marcadores morfológicos (Soares, 2005;Mendonça et al, 2008;Cassimiro et al, 2009), isoenzimáticos (Gois et al, 2009) e moleculares (Silva, 2009;Santos & Oliveira, 2008).…”
Section: Introductionunclassified