Molecular Characterization of Canine Parvovirus-2 (Cpv-2) in Dogs in Egypt

Etman, Rabie; Safwat, Mahmoud S.; Khodeir, H

doi:10.17582/journal.aavs/2021/9.6.933.940

Cited by 2 publications

(3 citation statements)

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“…CPV-2c was the predominant variant circulating in domestic dogs in Egypt, in contrast to earlier reports that accounted for a limited circulation of CPV-2c in this country (28,53). The CPV-2c mutant detected has been also reportedly spreading in Europe, Asia, and Africa (54)(55)(56)(57)(58)(59)(60)(61)(62)(63).…”

Section: Discussionmentioning

confidence: 54%

“…Both CPV-2a strain EGY/2019/dog/39-200 (OM937913) and CPV-2b strain EGY/2019/cat/39-517 (OM937914) displayed the mutation T440A and were closely related to the isolates from Turkey and Thailand (51,52). The T440A mutation was also prevalent in the CPV-2a strains previously identified in Egypt (53). CPV variants displaying VP2 changes F267Y, Y342I, and T440A are considered immune escape mutants, which are likely emerged due to vaccine pressure, with the role of the 267 mutation still unclear although it is an unexposed residue (37, 46).…”

Section: Discussionmentioning

confidence: 90%

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Molecular characterization of carnivore protoparvovirus 1 circulating in domestic carnivores in Egypt

Ndiana

Lanave²,

Zarea³

et al. 2022

Front. Vet. Sci.

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Canine parvovirus (CPV) and feline panleukopenia virus (FPV), now included in the unique species Carnivore protoparvovirus 1 (CPPV1), have been circulating in dogs and cats for several decades and are considered the causes of clinically important diseases, especially in young animals. While genetic evidence of the circulation of parvoviruses in Egyptian domestic carnivores has been provided since 2016, to date, all available data are based on partial fragments of the VP2 gene. This study reports the molecular characterization of CPPV strains from Egypt based on the full VP2 gene. Overall, 196 blood samples were collected from dogs and cats presented at veterinary clinics for routine medical assessment in 2019 in Egypt. DNA extracts were screened and characterized by real-time PCR. Positive samples were amplified by conventional PCR and then were sequenced. Nucleotide and amino acid changes in the sequences were investigated and phylogeny was inferred. Carnivore protoparvovirus DNA was detected in 18 out of 96 dogs (18.8%) and 7 of 100 cats (7%). Phylogenetic analyses based on the full VP2 gene revealed that 9 sequenced strains clustered with different CPV clades (5 with 2c, 2 with 2a, 1 with 2b, and 1 with 2) and 1 strain with the FPV clade. All three CPV variants were detected in dog and cat populations with a predominance of CPV-2c strains (7 of 18, 38.9%) in dog samples, thus mirroring the circulation of this variant in African, European, and Asian countries. Deduced amino acid sequence alignment revealed the presence of the previously unreported unique mutations S542L, H543Q, Q549H, and N557T in the Egyptian CPV-2c strains.

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 90%

Molecular characterization of carnivore protoparvovirus 1 circulating in domestic carnivores in Egypt

Ndiana

Lanave²,

Zarea³

et al. 2022

Front. Vet. Sci.

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“…The genotypes of the CPV-2 strains were previously investigated in Egypt. Abdel-Rhman et al [ 42 ], Awad et al [ 43 ], Al-hosary et al [ 21 ], and Etman et al [ 44 ] reported that CPV-2a and CPV-2b are the predominant viruses isolated from infected dogs in Egypt. Zaher et al [ 18 ] and Elbaz et al [ 20 ] previously recorded the CPV-2c genotype in Egypt, while Magouz et al [ 34 ] in a neighboring governorate to the study area indicated that only CPV-2a and CPV-2c were circulating in that area.…”

Section: Discussionmentioning

confidence: 99%

Epidemiological, and molecular investigation of Canine parvovirus-2 infection in Egypt

Ammar,

Hegazy,

Al-gaabary

et al. 2024

J Vet Sci

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Importance Canine parvovirus enteritis (CPE) is a contagious viral disease of dogs caused by the canine parvovirus-2 (CPV-2) associated with high morbidity and mortality rates. CPV-2 has a high global evolutionary rate. Molecular characterization of CPV-2 and understanding its epidemiology are essential for controlling CPV-2 infections. Objective This study examined the risk factors and survival outcomes of dogs infected with CPV-2. Molecular characterization of CPV-2 genotypes circulating in Egypt was performed to determine the evolution of CPV-2 nationally and globally. Methods An age-matched case-control study was conducted on 47 control and 47 CPV-infected dogs. Conditional logistic regression analysis examined the association between the potential risk factors and CPE in dogs. Survival analysis was performed to determine the survival pattern of the infected dogs. Thirteen fecal samples from infected dogs were collected to confirm the CPV genotype by CPV-2 VP2 gene sequencing, assembly of nucleotide sequences, and phylogenic analysis. Results Unvaccinated and roamer dogs had eight and 2.3 times higher risks of CPV infection than vaccinated dogs and non-roamer dogs, respectively. The risk of death from CPE was high among dogs without routine visits to veterinary clinics and among non-roamer dogs. Molecular characterization of CPV-2 confirmed its genotype identity and relationship with the CPV-2 c and b clade types. Conclusions and Relevance This study highlights the potential factors for CPE control, especially vaccination and preventing dogs from roaming freely outside houses. Isolated CPV genotypes are closely related to southern Asian genotypes, suggesting a substantial opportunity for global transmission.

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Molecular Characterization of Canine Parvovirus-2 (Cpv-2) in Dogs in Egypt

Cited by 2 publications

References 0 publications

Molecular characterization of carnivore protoparvovirus 1 circulating in domestic carnivores in Egypt

Molecular characterization of carnivore protoparvovirus 1 circulating in domestic carnivores in Egypt

Epidemiological, and molecular investigation of Canine parvovirus-2 infection in Egypt

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