Molecular characterization of carbapenem-resistant &lt;i&gt;Acinetobacter baumannii&lt;/i&gt; isolated from pediatric burns patients in an Iranian hospital

Pournajaf, Abazar; Rajabnia, Ramazan; Razavi, Shabnam; Solgi, Sana; Ardebili, Abdollah; Yaghoubi, Sajad; Khodabandeh, Mahmoud; Yahyapour, Yousef; Emadi, Behzad; Irajian, Gholamreza

doi:10.4314/tjpr.v17i1.19

Cited by 21 publications

(14 citation statements)

References 16 publications

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“…Following this, 10 µL of MBL inhibitor (iMBL; 0.5 M EDTA) was directly added to one of the disks to reach a desired concentration of 750 mg. After an overnight incubation period at 37 °C, inhibition zone diameter (IZD) of all disks was recorded and compared. The strains were recognized as an MBL-producing isolate when the difference in the IZD was ≥ 7 mm [15].…”

Section: Phenotypic Detection Of Mblsmentioning

confidence: 99%

Correlation between antimicrobial resistance and biofilm formation capability among Klebsiella pneumoniae strains isolated from hospitalized patients in Iran

Shadkam

Goli

Mirzaei

et al. 2021

Ann Clin Microbiol Antimicrob

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Background Klebsiella pneumoniae is a common cause of nosocomial infections. Antibiotic resistance and ability to form biofilm, as two key virulence factors of K. pneumoniae, are involved in the persistence of infections. The purpose of this study was to investigate the correlation between antimicrobial resistance and biofilm formation capability among K. pneumoniae strains isolated from hospitalized patients in Iran. Methods Over a 10-month period, a total of 100 non-duplicate K. pneumoniae strains were collected. Antibiotic susceptibility was determined by Kirby–Bauer disk diffusion method according to CLSI. Biofilm production was assessed by tissue culture plate method. Finally, polymerase chain reaction was conducted to detect four families of carbapenemase: blaIMP, blaVIM, blaNDM, blaOXA−48; biofilm formation associated genes: treC, wza, luxS; and K. pneumoniae confirming gene: rpoB. Results Most of the isolates were resistant to trimethoprim-sulfamethoxazole (52 %), cefotaxime (51 %), cefepime (43 %), and ceftriaxone (43 %). Among all the 100 isolates, 67 were multidrug-resistant (MDR), and 11 were extensively drug-resistant (XDR). The prevalence of the blaVIM, blaIMP, blaNDM, and blaOXA−48 genes were 7 , 11 , 5 , and 28 %, respectively. The results of biofilm formation in the tissue culture plate assay indicated that 75 (75 %) strains could produce biofilm and only 25 (25 %) isolates were not able to form biofilm. Among these isolates, 25 % formed fully established biofilms, 19 % were categorized as moderately biofilm-producing, 31 % formed weak biofilms, and 25 % were non-biofilm-producers. The antimicrobial resistance among biofilm former strains was found to be significantly higher than that of non-biofilm former strains (p < 0.05). Molecular distribution of biofilm formation genes revealed that 98 , 96 , and 34 % of the isolates carried luxS, treC, and wza genes, respectively. Conclusions The rise of antibiotic resistance among biofilm-producer strains demonstrates a serious concern about limited treatment options in the hospital settings. All of the data suggest that fundamental actions and introduction of novel strategies for controlling of K. pneumoniae biofilm-related infections is essential.

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Section: Phenotypic Detection Of Mblsmentioning

confidence: 99%

Correlation between antimicrobial resistance and biofilm formation capability among Klebsiella pneumoniae strains isolated from hospitalized patients in Iran

Shadkam

Goli

Mirzaei

et al. 2021

Ann Clin Microbiol Antimicrob

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“…brane permeability, modifies PBP, and increases efflux pump expression. Carbapenem resistance in A. baumannii is mainly mediated by intrinsic (OXA-51) or acquired (OXA-23) oxacillinases (9,10). It is considered a health care pathogen mostly encountered in several serious medical problems such as septicemia, meningitis, bacteremia, ventilator-associated pneumonia, endocarditis, and urinary tract infection (4,11).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and Genotypic Evaluation of Antibiotic Resistance of Acinetobacter baumannii Bacteria Isolated from Surgical Intensive Care Unit Patients in Pakistan

Zahra

Zeshan

Qadri

et al. 2021

Jundishapur J Microbiol

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Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) is a significant nosocomial pathogen, causing serious threats concerning community-wide outbreaks globally, as well as in Pakistan. Antimicrobial resistance in A. baumannii is increasing day by day. Objectives: The study aimed to find out the antibiotic resistance (AMR) patterns and evaluate the AMR genes in clinical isolates from patients admitted to the surgical Intensive Care units (ICUs) at different hospitals in Lahore, Pakistan. Methods: A total of 593 clinical specimens were collected from patients admitted to the surgical ICUs of three different local hospitals in Lahore, Pakistan. From these samples, a total of 90 A. baumannii isolates were identified and further investigated to observe phenotypic resistance patterns and detect carbapenemases resistance genes. Results: The results showed that phenotypic resistance against amikacin was 27.2%, ceftriaxone 100%, ceftazidime 27.2%, cefepime 63.3%, ciprofloxacin and co-trimoxazole 100%, gentamicin 40%, imipenem 22.2%, meropenem 21.1%, piperacillin-tazobactam 27.2%, tigecycline 27.2%, and tetracycline 63.3%. All A. baumannii isolates were found to be sensitive to colistin (CT), polymixin-B (PB), and tobramycin (TOB). The PCR amplification of carbapenemases genes revealed the prevalence of blaOXA-23, blaOXA-51, and blaOXA-40 in 73, 90, and 64.4% of the isolates, respectively, along with blaNDM1 (92.2%), blaVIM (40%), blaIMP (90%), ISAba1 (85.5%), sul1 (16.6%), sul2 (20%), armA (32.2%), and PER-1 (12%) while the blaOXA-24 and blaOXA-58 genes were not detected in the isolates. The sequence analysis of the blaOXA-23 and blaOXA-51 genes showed 98% and 95% similarity with previously reported sequences in the GenBank database. Conclusions: The present study indicated that the emergence of high carbapenem resistance in CRAB isolates has increased, which may pose serious limitations in the choice of drugs for nosocomial infections.

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“…Acinetobacter baumannii is an opportunistic microorganism that has emerged as one of the most troublesome pathogens worldwide 1,2 . This pathogen is responsible for severe infections, such as ventilator-associated pneumonia (VAP), bloodstream, urinary tract, and wound infections 3,4 .…”

Section: Introductionmentioning

confidence: 99%

Tetracycline resistance mediated by tet efflux pumps in clinical isolates of Acinetobacter baumannii

Beheshti

Ardebili

Beheshti

et al. 2020

Rev. Inst. Med. trop. S. Paulo

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Molecular characterization of carbapenem-resistant <i>Acinetobacter baumannii</i> isolated from pediatric burns patients in an Iranian hospital

Cited by 21 publications

References 16 publications

Correlation between antimicrobial resistance and biofilm formation capability among Klebsiella pneumoniae strains isolated from hospitalized patients in Iran

Correlation between antimicrobial resistance and biofilm formation capability among Klebsiella pneumoniae strains isolated from hospitalized patients in Iran

Phenotypic and Genotypic Evaluation of Antibiotic Resistance of Acinetobacter baumannii Bacteria Isolated from Surgical Intensive Care Unit Patients in Pakistan

Tetracycline resistance mediated by tet efflux pumps in clinical isolates of Acinetobacter baumannii

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