Molecular Characterization of Carbapenemase Encoding Genes in Pseudomonas aeruginosa from Tertiary Healthcare in South Eastern Nigeria

Ogba, Rebecca Chinenye; Nomeh, Onyinye Lovette; Edemekong, Christiana Inuaesiet; Nwuzo, Agabus Chidiebube; Akpu, Peace Oluchi; Peter, Ikemesit Udeme; Iroha, Ifeanyichukwu Romanus

doi:10.9734/ajbgmb/2022/v12i4281

Cited by 7 publications

(23 citation statements)

References 27 publications

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“…The World Medical Association (WMA) declaration required that all subject data be classified for confidentiality. Standard microbiological techniques were used to identify and characterize the 10 clinical isolates of E. coli and K. pneumoniae (Ogba et al, 2022).…”

Section: Materials and Methods 1 E Coli And K Pneumoniae Characteriza...mentioning

confidence: 99%

Urinary Tract Infection Caused by Carbapenemase-Producing K. pneumoniae and E. coli at the Institute of Kidney Disease Peshawar, Pakistan

Hariri

Khan

2023

Egyptian Academic Journal of Biological Sciences. C, Physiology

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Aims: Carbapenemase-producing bacteria make infections of the urinary tract (UTIs) challenging to cure with last-resort treatment like carbapenem. Carbapenemase-producing E. coli and K. pneumoniae implicated in UTI must be detected molecularly since their ability to spread broadly among patients is rising ( Nomeh et al., 2022). Methodology: Ten non-repeated clinical isolates of Escherichia coli (Ecoli1, Ecoli2, Ecoli3, Ecoli4, and Ecoli5) and Klebsiella pneumoniae (Kp6, Kp7, Kp8, Kp9, Kp10) were selected from urinary tract infection patients at Institute of Kidney Disease Peshawar, Pakistan, based on their in vitro phenotypic carbapenem antibiotic resistance. These isolates were confirmed using standard routine microbiological techniques. PCR-specific primers screened E. coli and K. pneumoniae strains for Carbapenemase-producing genes. Result: Molecular Detection of Carbapenemase-producing Gene in UTI Patients with Uropathogenic Escherichia coli and Klebsiella pneumoniae. The higher proportion of Carbapenemase-producing genes in all the bacterial isolates in this study was blaKPC 15(100 %), followed by blaNDM 12.3(90.1 %), blaIMP 6(60.2 %) and blaVIM 3(30.6 %). The most common Carbapenemase gene in Escherichia coli 8 (80%) was blaKPC, followed by blaNDM 7 (70%) and blaOXA 45 (4.5%), which was the least common. Klebsiella pneumoniae had more blaNDM and blaKPC than blaOXA. Both had a percentage of 4.5 (40.9%). Conclusion:These results are consistent with the rapid spread of genes responsible for generating Carbapenemases in E. coli and Klebsiella pneumoniae that cause urinary tract infections. Despite the lack of blaVIM in K. pneumoniae, the pathogenic function of Carbapenemase-producing genes in UTI in this study should not be underestimated because of the potential they have to cause treatment failure and the subsequent persistence of UTI in patients.

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Section: Materials and Methods 1 E Coli And K Pneumoniae Characteriza...mentioning

confidence: 99%

Urinary Tract Infection Caused by Carbapenemase-Producing K. pneumoniae and E. coli at the Institute of Kidney Disease Peshawar, Pakistan

Hariri

Khan

2023

Egyptian Academic Journal of Biological Sciences. C, Physiology

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Section: Introductionmentioning

confidence: 99%

“…Carbapenemase producers are isolates with resistance to cephamycins and carbapenems via the expression of a variety of genes including KPC, OXA-48, IMP, VIM, and NDM 9,10,11 . (Nordmann et al, 2011;Ogba et al, 2022a;Nomeh et al, 2023). The intestinal microbiota, feces, and rectal swabs are all typical reservoirs of carbapenemase production in hospital settings 12 .…”

Section: Introductionmentioning

confidence: 99%

Comparison of Antibiotic-Resistant Pattern of Extended Spectrum Beta-Lactamase and Carbapenem-Resistant Escherichia Coli Isolates from Clinical and Non-Clinical Sources

Joseph,

Okolo,

Udenweze

et al. 2023

J. Drug Delivery Ther.

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The increasing rate of antibiotic resistance among E. coli especially those mediated by extended spectrum beta-lactamases and carbapenem-resistant (CR) presents a major threat to public health and healthcare delivery globally. The aim of this research was to compare the antibiotic resistant pattern of extended spectrum beta-lactamase and carbapenem-resistant Escherichia coli isolates from clinical and non-clinical sources. A total of two hundred and fifteen (215) clinical and non-clinical samples were collected for the study. The collected samples were analysis using Standard Microbiological protocol for isolation and identification. Phenotypic detection of ESBL Production and carbapenem resistant was performed using Double Disk Synergy Test (DDST) and Modified Hodge Test (MHT) respectively. Antibiogram studies of ESBL producing and Carbapenem-resistant E. coli was determined using the Kirby–Bauer disk diffusion method. The result of isolation and characterization revealed higher occurrence rate of clinical isolates of E. coli 66.1 % over non-clinical sample 54.0%. Phenotypic ESBL screening of isolated E. coli revealed overall detection rate of 35(30.4 %) and 13(13.0 %) in clinical and non-clinical source respectively while overall detection rate of carbapenem resistant ESBL producers accounted 9.6 % and 8.0 % in clinical and non-clinical isolates respectively. The isolates exhibited high percentage of resistance to nitrofurantion 100 %, cefepime 100 % colistin 66.7 %, amikacin 50.0 % and also exhibit MDR with MARI value of ≥ 0.5. Comparison of antibiotic resistant pattern of carbapenem-resistant extended spectrum beta-lactamase producing E. coli isolates from clinical and non-clinical sources showed no statistical significant difference P-value < 0.05 but were 100 % susceptible to ciprofloxacin and gentamicin. The pattern of similar MDR portrayed by clinical and non-clinical isolate should not be overlooked due to its hyper-motility and virulence domination leading to possible AMR transfer to other bacterial organisms and also, appropriate measures such as pretreatment of animal excrement before being used as fertilizers and the quality of irrigation water, hospital effluent discharge need to be taken into consideration to prevent the spread of resistant determinant. Keywords: Escherichia coli, Extended Spectrum Beta-Lactamase, Carbapenem-Resistant, clinical, non-clinical

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“…Escherichia coli and Pseudomonas aeruginosa are Gramnegative rods associated with health care, and are significant causes for infection with antibiotic resistance burden 1,2,3,4,5 Mustafai et al, 2023). The treatment of human infection associated with this pathogen are commonly with beta-lactam antimicrobials agent.…”

Section: Introductionmentioning

confidence: 99%

“…Most Escherichia coli and Pseudomonas aeruginosa resistance to beta-lactams is mainly due to the production of beta-lactamases enzymes, which are often encoded either on the chromosome or plasmid 2, 3, 4, (Ogba et al, 2022;Nomeh et al, 2023;Egwu et al, 2023). Beta-lactamases, such as carbapenemase betalactamases and extended-spectrum beta-lactamases (ESBLs), are increasingly being identified in food and companion animals, wildlife, humans, and the environment around the world 3,4,7,8 Joseph et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Characterization of VIM, VEB and CTX-M Beta-lactamase Gene in Escherichia coli and Pseudomonas aeruginosa Isolated from Urine Samples of Patients Visiting a Tertiary Hospital in Abakaliki

Ilang,

Peter,

Iroha

2023

Int J Med Sci Pharm Res

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The spread and convergence of multiple beta-lactamase genes across distinct resistant bacterial populations from various hosts and settings demonstrates increased risk of morbidity and mortality in humans. This study was undertaken to characterize blaVIM, blaVEB and blaCTX-M beta-lactamase gene in Escherichia coli and P. aeruginosa isolates from patients visiting a tertiary hospital in Abakaliki. A total of three hundred (300) urine samples were collected from patients and were subjected to bacteriological examination using culture, Gram staining and biochemical technique, for routine microbiological identification and further confirmed using the VITEK-2 Automated System (Biomerieux, France). Antimicrobial susceptibility studies were determined using the Kirby–Bauer disk diffusion method. All isolate were further screen for various beta-lactamase resistant gene by PCR using specific primer. Of the 300 urine samples collected, prevalence rate of 187 (62.3%) and 91 (30.3 %) E. coli and P. aeruginosa were recorded. The isolates exhibited 50.0-100% percentage of resistance to Amoxycillin-Clavulanic acid, Azetronam, Cefoxitin, Ceftriaxone and Piperacillin/tazobactam. The proportion of beta-lactamase gene in E. coli were as follows (VEB 143/76.5 %; CTX-M 175/93.5 %; VIM 77/41.2 %) while beta-lactamase gene in P. aeruginosa were as follows (VEB 91/100 %; CTX-M 63/69.2%; VIM 48/52.7 %). The presence of these gene in our study indicates the possibility of therapeutic failure, serious consequences for infection control and increased risk of morbidity and mortality in patients. Hence, continuous effort in hospital surveillance, infection control, and clinical audits must be conducted to fight against the rapid development and spread of antibiotic-resistant bacteria pathogens. Keywords: Beta-lactamase, Escherichia coli, Pseudomonas aeruginosa, VIM, VEB, CTX-M

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Molecular Characterization of Carbapenemase Encoding Genes in Pseudomonas aeruginosa from Tertiary Healthcare in South Eastern Nigeria

Cited by 7 publications

References 27 publications

Urinary Tract Infection Caused by Carbapenemase-Producing K. pneumoniae and E. coli at the Institute of Kidney Disease Peshawar, Pakistan

Urinary Tract Infection Caused by Carbapenemase-Producing K. pneumoniae and E. coli at the Institute of Kidney Disease Peshawar, Pakistan

Comparison of Antibiotic-Resistant Pattern of Extended Spectrum Beta-Lactamase and Carbapenem-Resistant Escherichia Coli Isolates from Clinical and Non-Clinical Sources

Characterization of VIM, VEB and CTX-M Beta-lactamase Gene in Escherichia coli and Pseudomonas aeruginosa Isolated from Urine Samples of Patients Visiting a Tertiary Hospital in Abakaliki

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