Molecular characterization of chlorpyrifos degrading bacteria isolated from contaminated dairy farm soils in Nakuru County, Kenya

Asamba, Micah Nyabiba; Njeru, Ezekiel Mugendi; Sifuna, Oshule Paul; Essuman, Suliman; Chimbevo, Lenny Mwagandi; Norbert, Atego

doi:10.1016/j.heliyon.2022.e09176

Cited by 10 publications

(4 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the four strains, CNN1 had the lowest OD620 value, and it was reported at 0.14 after 48 h and 0.25 after 96 h. Based on the results, the strains CNN2, VNN3 and CNN4 were selected for further experiment and analysis. The results based on the OD620 value are comparable to those recorded by Asamba et al (2022) who reported that OD600 value of the six isolated CPF degrading bacterial strains was from 0.05 to 0.25 after five days. The values of bacterial density in the present study were lower than the previous research, which reported that the density of the bacterial CPF-degrading strain Hortaea sp.…”

Section: Isolation Of Cpf-degrading Bacteriasupporting

confidence: 87%

See 1 more Smart Citation

Isolation and characterization of chlorpyrifos-degrading bacteria in tea-growing soils

Nguyen¹,

Nguyen²,

Thanh³

et al. 2023

J Exp Bio & Ag Sci

View full text Add to dashboard Cite

The excess use of pesticides in the agricultural sector has caused environmental pollution and affected the complete ecosystem. Among the various commonly used pesticides, chlorpyrifos (CPF) is widely used against multiple agrarian pests due to its effectiveness and higher insecticidal activities. However, along with its beneficial usage, CPF has various residual effects on the environment, causing multiple negative impacts on aquatic organisms and human health. Consequently, methods for eliminating CPF in the background are essential. Among the currently available approaches to CPF remediation, biological methods using microorganisms are eco-friendly and cost-effective. Therefore, this study was conducted to isolate and characterize chlorpyrifos-degrading bacteria from the tea-growing soil of Vietnam. For this, soil samples were collected from the 20 tea-growing areas of Vietnam. From the collected samples, three bacterial strains viz., Methylobacterium populi CNN2, Ensifer adhaerens VNN3, and Acinetobacter pittii CNN4 have been isolated by using streak plate method and identified based on 16S rRNA gene analysis. The study results showed that under laboratory conditions, E. adhaerens VNN3 had the highest CPF degradation ability and was followed by the strain M. populi CNN2. In liquid medium, CPF concentration (100 mg/L) was reduced by 95.2% and 81.4% by E.adhaerens VNN3 and M. populi CNN2, respectively, after 72 h. Further, under in-vitro conditions, the concentration of CPF was reduced from 500 mg/kg to 112 ± 1.73 (77.6%) and 197 ± 2.08 mg/kg (60.6%) by E. adhaerens VNN3 and M. populi CNN2, respectively. Based on the obtained results, it can be concluded that E. adhaerens VNN3 and M. populi CNN2 can be used for CPF-contaminated agricultural soil remediation.

show abstract

Section: Isolation Of Cpf-degrading Bacteriasupporting

confidence: 87%

“…The colonies of CNN4 were ivory white, round and small, smooth surface and non-pigmentated (diameter: 1.5 mm). In a previous study, Asamba et al (2022) found that colonies of six CPF-degrading bacterial strains isolated from contaminated soils in Kenya were rod-shaped and pigmented.…”

Section: Isolation Of Cpf-degrading Bacteriamentioning

confidence: 96%

Isolation and characterization of chlorpyrifos-degrading bacteria in tea-growing soils

Nguyen¹,

Nguyen²,

Thanh³

et al. 2023

J Exp Bio & Ag Sci

View full text Add to dashboard Cite

show abstract

“…Bacillus flexus was reported for phosphorus solubilization, degradation of pyrethroids, and polyaromatic hydrocarbons (Gousia Gani et al 2021). Likewise, association of Pseudomonas with alkaline phosphatase, urease, and N-acetyl glucosaminidase suggested their involvement in the mineralization of C, N, and P (Asamba et al 2022;Singh et al 2022b). The role of Pseudomonas sp.…”

Section: Effect Of Biochar On Microbial Community Enzymes and Degrada...mentioning

confidence: 99%

Effect of biochar on soil microbial community, dissipation and uptake of chlorpyrifos and atrazine

Singh,

Yadav,

Pandey

et al. 2024

Biochar

View full text Add to dashboard Cite

For the application of biochar in restoring pesticide-contaminated soils and minimizing the risk associated with their uptake in plants, it is crucial to understand the biochar impact on soil biological activities and dissipation and accumulation of pesticides in plant and soil systems. In this study, the effect of Mentha-distilled waste-derived biochar was investigated on chlorpyrifos and atrazine contaminated sandy loam soil. The four application rates of atrazine (2, 4, 6, and 8 mg kg−1) and chlorpyrifos (2, 4, 6, and 12 mg kg−1) and a single application rate of biochar (4%) were used in this study. The degradation of pesticides, the diversity of the bacterial community, and enzymatic activities (alkaline phosphatase, dehydrogenase, arylsulfatase, phenol oxidase, urease activity and N-acetyl glucosaminidase) were examined in soil. The uptake of two pesticides and their effect on growth and stress parameters were also investigated in plants (A. paniculata). The dissipation of chlorpyrifos and atrazine followed simple first-order kinetics with a half-life of 6.6–74.6 and 21–145 days, respectively. The presence of deisopropyl atrazine desethyl atrazine (metabolites of atrazine) and 3,5,6-trichloro-2-pyridinol (a metabolite of chlorpyrifos) was observed in soil and plant tissues. Biochar application significantly (p = 0.001) enhanced the degradation rate of chlorpyrifos and atrazine leading to the lower half-life of chlorpyrifos and atrazine in soil. A significant reduction (p = 0.001) in the uptake of chlorpyrifos and atrazine and alteration in their binding affinity and uptake rate in plant tissues was observed in biochar treatments. The incorporation of biochar improved chlorpyrifos/atrazine degrader and plant growth-promoting bacterial genera such as Balneimonas, Kaistobacter, Rubrobacter, Ammoniphilus, and Bacillus. The upregulation of functional genes associated with nucleotide, energy, carbohydrate, amino acid metabolism, xenobiotic biodegradation, and metabolism: atrazine degradation was observed in biochar treatments. The biochar amendments significantly (p = 0.001) reduced the plant’s uptake velocity (Vmax) and affinity (Km) of chlorpyrifos and atrazine. These results delineated that Mentha-distilled waste-derived biochar can potentially remediate chlorpyrifos and atrazine contaminated soils and ensure the safety of plants for consumption. Graphical Abstract

show abstract

“…The removal of various categories of pesticides has recently been explained in great detail using a variety of methods, including microfiltration (Doulia et al, 2016), bio-purification systems containing bio mixtures of coconut fibre, compost, and soil (HueteSoto et al, 2017), and conductive diamond photoelectrochemical oxidation (Rubí-Juárez et al, 2016). Additionally, a particular class of Gram-positive bacteria known as actinobacteria have the capacity to extensively break down organophosphorus insecticides (Asamba et al, 2022). Due to their mycelial growth, relatively quick growth rates, capacity to colonise semiselective substrates, vulnerability to genetic manipulation, and ability to produce surfactants, strains of the Streptomyces genus are widely used for the bioremediation of environments contaminated with organophosphorus pesticides.…”

Section: Implications For Human Healthmentioning

confidence: 99%

Organophosphate Pesticide Residues in Food Products in Kenya and their Chromatographic Detection: A Systematic Review

Asamba

Wepukhulu

2023

East Afr. j. agric. biotechnol.

View full text Add to dashboard Cite

Organophosphate pesticides are used worldwide to control several pests and meet food demand. These chemicals harm non-target animals and people when misused. Thus, they are a health and environmental concern. The purpose of the systematic review was to synthesise the amount, breadth, and quality of evidence from empirical studies concerning the presence, type, and quantity of OPs in food products in Kenya. A systematic review was done by following the PRISMA protocol. For the identification of studies, the following databases were used: Google Scholar, Web of Science, Scopus, and PubMed. The study focused on peer-reviewed articles published between January 2001 and August 2022. Twelve studies met the inclusion criteria for the scoping review. The main methods used in detecting and quantifying organophosphates in the studies were High-Performance Liquid Chromatography (HPLC) and Gas Chromatography-Mass Spectrometry (GC-MS). Most studies (86.67%) reported significant levels of OPs in food products, exceeding the MRLs and the Acceptable Daily Intake (ADIs) set by the World Health Organization. Six of the studies (50%) reported the presence of OPs in plant products (vegetables, cereals, and fruits), while three (25%) found residues in animal products (milk and meat). The most notable OP compounds detected were chlorpyrifos, acephate, profenofos, diazinon, omethoate, and dimethoate. Most of the food samples in the reviewed studies presented contamination, making them a significant risk to human health due to bioaccumulation. Studies done in Kenya for the last 20 years continue to report high levels of organophosphate residues and their metabolites in food products, both from plant and animal origin. Although the residues are below MLRs in some samples, they can accumulate at higher levels in humans, becoming a severe health risk

show abstract

Molecular characterization of chlorpyrifos degrading bacteria isolated from contaminated dairy farm soils in Nakuru County, Kenya

Cited by 10 publications

References 37 publications

Isolation and characterization of chlorpyrifos-degrading bacteria in tea-growing soils

Isolation and characterization of chlorpyrifos-degrading bacteria in tea-growing soils

Effect of biochar on soil microbial community, dissipation and uptake of chlorpyrifos and atrazine

Organophosphate Pesticide Residues in Food Products in Kenya and their Chromatographic Detection: A Systematic Review

Contact Info

Product

Resources

About