Molecular characterization of class 1 integrons in carbapenem-resistant Enterobacterales isolates

Wang, Tong; Zhu, Yu; Zhu, Wenwen; Cao, Mei; Wei, Q.

doi:10.1016/j.micpath.2023.106051

Cited by 10 publications

(7 citation statements)

References 38 publications

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Section: Discussionsupporting

confidence: 60%

“…Reportedly, not just E. coli but Enterobacteriaceae family as a whole has been reported with higher AMR specifically Ampicillin (100%), Ampicillin-sulbactam (100%), Cefazolin (100%), Levofloxacin (97%) and Ciprofloxacin (96%) within the integron-positive population in China. 25 Besides China, similar high resistance has been reported with integron-positive population from a hospital of Pakistan 23 where higher resistance was shown towards Amoxicillin-clavulanic acid (100%), Ceftazidime (94%) and Cefotaxime (94%). Evidently, it is believed that overall antibiotic susceptibility will likely be high in bacteria if they do not have integrons but the frequency of integron negative population of this study is very low, therefore the relation between AMR and integron-population could not be reported with certainty in this study.…”

Section: Discussionsupporting

confidence: 60%

“…24 However, majority of the isolates (79.2%) were positive for NDM-1, followed by OXA-23, OXA-48, and VIM within the integron-positive population. The dominance of NDM-1 is not surprising, given that it is the most common carbapenemase gene that is found in E. coli [24][25][26][27] and; NDM-1 is particularly associated with class 1 integrons gene cassette and IncM plasmids which help them to mobilize. 34 So the co-occurrence on NDM-1 with intI1 as well as with other OXA variants is concerning as they can transfer the antibiotic resistant trait by moving between species.…”

Section: Discussionmentioning

confidence: 99%

“…In this study, the prevalence of the Class-1 integron gene, intI1, among CREc isolates was found be high -92% (n=48/52). Though not similar, a varying prevalence percentage of class 1 integrons has been well documented within multidrug-resistant E. coli from humans, 10,[23][24][25][26][27][28][29] animals, [29][30] and the environment. 7,[13][14][15] One possible reason for such high prevalence may be that integrons occur widely in Enterobacteriaceae family, and are strongly correlated with MDR.…”

Section: Discussionmentioning

confidence: 99%

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Prevalence of Class 1 integron in disease-causing carbapenem-resistantEscherichia coliin a tertiary hospital in Kathmandu, Nepal

Thapa,

Shrestha,

Shrestha

et al. 2024

Preprint

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BackgroundHigh prevalence of carbapenem resistance genes have been abundantly reported within clinicalE. coliin Nepal but their co-prevalence with class-1 integron is poorly documented. These Class I Integrons play pivotal role in evolution and spread of antimicrobial resistance (AMR). Therefore, it is necessary to highlight their epidemiological significance, so this study determined the prevalence of Class 1 integrons within Carbapenem resistanceEscherichia coli(CREc) population.MethodsFifty-two clinicalE. coliwith confirmed resistance to Meropenem were collected between January 2020–April 2022 in Kathmandu Model Hospital, and undergone antimicrobial susceptibility testing for 11 antimicrobial classes. Carbapenemase production was confirmed by eCIM and mCIM methods. Presence of Class I integron and carbapenemase genes,blaNDM-1,blaKPC,blaIMP,blaVIM,blaOXA-23,blaOXA-48, was confirmed by PCR method.ResultsThe result revealed 92.3% (n=48/52) of the isolates harboredintI1gene. Among integron-positive population, 97.9% (n=47/48) were carbapenemase producers which were further identified as metallo β-lactamase (MBL) producers (82.9%, n=39/47) and serine β-lactamase (SBL) producer (17%, n=8/47). Carbapenemase geneblaNDM-1(79.2%, n=38/48) was highly prevalent within integron-positive isolates, followed byblaOXA-23(62.5%, n=30/48) andblaOXA-48(54.2%, n=26/48). All integron-positive CREc isolates were resistant to minimum 16 antibiotics tested, but were susceptible to Colistin, Polymixin B, and Tigecycline.ConclusionIn conclusion, Class 1 integrons were highly prevalent within clinical CREc in Nepal, and their co-prevalence with other CRE gene is concerning due to possibility of wider spread. Therefore, an in-depth genomic analysis of Class 1 integrons is needed to understand the integron mediated AMR in high risk clones like CREc and inform intervention measures to stop spread of such pathogens.

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Section: Discussionsupporting

confidence: 60%

Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Prevalence of Class 1 integron in disease-causing carbapenem-resistantEscherichia coliin a tertiary hospital in Kathmandu, Nepal

Thapa,

Shrestha,

Shrestha

et al. 2024

Preprint

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“…The supernatant was stored at -20°C for further use. Primers for intI and variable regions were synthesized according to references [12][13][14][15], and the primer sequences are shown in Table 1. The PCR reaction for intI and the 3' end was performed as follows: initial denaturation at 94°C for 3 minutes, followed by 35 cycles of denaturation at 94°C for 30 seconds, annealing at the temperature specified in Table 1 for 30 seconds, and extension at 72°C for 60 seconds.…”

Section: Pcr Amplification Experimentsmentioning

confidence: 99%

The relationship between integrons, antibiotic resistance genes and SXT resistance in Shigella flexneri strains

Peng

2024

Am J Transl Res

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Objective: To investigate the correlation between sulfamethoxazole-trimethoprim (SXT) resistance in Shigella flexneri (S.flexneri) and the presence of integrons and relevant antibiotic resistance genes. Methods: We collected 115 strains of Shigella flexneri isolated from feces of children with diarrhea in Jinan from 2012 to 2020 and determined the minimum inhibitory concentration (MIC) of SXT by Etest method. The presence of class 1, class 2, and class 3 integron genes, variable region antibiotic resistance gene cassettes, and sul1, sul2, sul3, and SXT elements were detected using polymerase chain reaction (PCR). Positive results were further analyzed by DNA sequencing and BLAST comparison. Results: In total, the resistance rate to SXT was 60.9% among the 115 S.flexneri strains. The prevalence of class 1 and class 2 integrons were 88.7% and 87.0%, respectively, with no class 3 integrons detected. Among the strains, 13.0% carried typical class 1 integrons with variable region antibiotic resistance gene cassettes dfrA17-aadA5 and dfrV, while 85.2% carried atypical class 1 integrons with variable region antibiotic resistance gene cassette blaoxa-30-aadA1. The variable region antibiotic resistance gene cassettes of class 2 integrons were all dfrA1+sat1+aadA1. There was a statistical difference between the presence of class 1 integrons and class 2 integrons between the SXT-sensitive and resistant S.flexneri strains (χ 2 =22.800, χ 2 =16.365, P<0.01, P<0.01). Integrons carrying dfrV and dfrA1 by integrons also showed a statistical difference in SXT resistance (χ 2 =9.422, χ 2 =16.365, P<0.01, P<0.01). PCR revealed the presence of sul1 and sul2 in 13.0% and 47.0% of strains, respectively, with neither sul3 nor SXT elements detected. There was a significant difference between the presence of sul1, sul2 between the SXT-sensitive and resistant S.flexneri strains (χ 2 =9.588, χ 2 =65.445, P<0.01, P<0.01). Conclusion: In summary, integrons are involved in SXT resistance of S.flexneri, and dfrV, dfrA1, sul1, sul2 are closely related to SXT resistance of S.flexneri.

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Molecular Characterization of Class 1 Integrons and Carbapenem-Resistant Genes in Enterobacter cloacae Complex Isolates

Zhu,

Wang,

Zhu

et al. 2024

Curr Microbiol

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Molecular characterization of class 1 integrons in carbapenem-resistant Enterobacterales isolates

Cited by 10 publications

References 38 publications

Prevalence of Class 1 integron in disease-causing carbapenem-resistantEscherichia coliin a tertiary hospital in Kathmandu, Nepal

Prevalence of Class 1 integron in disease-causing carbapenem-resistantEscherichia coliin a tertiary hospital in Kathmandu, Nepal

The relationship between integrons, antibiotic resistance genes and SXT resistance in Shigella flexneri strains

Molecular Characterization of Class 1 Integrons and Carbapenem-Resistant Genes in Enterobacter cloacae Complex Isolates

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