Molecular characterization of class I histone deacetylases and their expression in response to thermal and oxidative stresses in the red flour beetle, Tribolium castaneum

Chen, Minxuan; Zhang, Nan; Jiang, Heng; Meng, Xiangkun; Qian, Kun; Wang, Jianjun

doi:10.1007/s10709-019-00065-3

Cited by 7 publications

(11 citation statements)

References 35 publications

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“…We detected higher levels of hdac1 transcripts in the beginning of larval development, followed by a decrease in the intermediate stage, and by a subsequent increase in the last larval stage. Similar profiles of mRNA relative expression levels of hdac1 had been previously described in Tribolium castaneum , in which HDAC1 expression is essential to larval survival 59 , 61 . In this beetle species, this gene presents higher transcripts levels in eggs and 1-day-old larvae and lower levels during larval development.…”

Section: Discussionsupporting

confidence: 79%

“…Expression of HDAC encoding genes have not been studied during development in bees, however they were extensively investigated in beetles 59 – 61 . We detected higher levels of hdac1 transcripts in the beginning of larval development, followed by a decrease in the intermediate stage, and by a subsequent increase in the last larval stage.…”

Section: Discussionmentioning

confidence: 99%

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10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees

Borges

Batista

Malta

et al. 2021

Sci Rep

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In bees from genus Melipona, differential feeding is not enough to fully explain female polyphenism. In these bees, there is a hypothesis that in addition to the environmental component (food), a genetic component is also involved in caste differentiation. This mechanism has not yet been fully elucidated and may involve epigenetic and metabolic regulation. Here, we verified that the genes encoding histone deacetylases HDAC1 and HDAC4 and histone acetyltransferase KAT2A were expressed at all stages of Melipona scutellaris, with fluctuations between developmental stages and castes. In larvae, the HDAC genes showed the same profile of Juvenile Hormone titers—previous reported—whereas the HAT gene exhibited the opposite profile. We also investigated the larvae and larval food metabolomes, but we did not identify the putative queen-fate inducing compounds, geraniol and 10-hydroxy-2E-decenoic acid (10HDA). Finally, we demonstrated that the histone deacetylase inhibitor 10HDA—the major lipid component of royal jelly and hence a putative regulator of honeybee caste differentiation—was unable to promote differentiation in queens in Melipona scutellaris. Our results suggest that epigenetic and hormonal regulations may act synergistically to drive caste differentiation in Melipona and that 10HDA is not a caste-differentiation factor in Melipona scutellaris.

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Section: Discussionsupporting

confidence: 79%

Section: Discussionmentioning

confidence: 99%

10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees

Borges

Batista

Malta

et al. 2021

Sci Rep

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show abstract

“…We detected higher levels of hdac1 transcripts in the beginning of larval development, followed by a decrease in the intermediate stage, and by a subsequent increase in the last larval stage. Similar profiles of mRNA relative expression levels of hdac1 had been previously described in Tribolium castaneum, in which HDAC1 expression is essential to larval survival [58,60] . In this beetle species, this gene presents higher transcripts levels in eggs and 1-day-old larvae and lower levels during larval development.…”

Section: Discussionsupporting

confidence: 78%

“…Expression of HDAC encoding genes have not been studied during development in bees, however they were extensively investigated in beetles [58][59][60] . We detected higher levels of hdac1 transcripts in the beginning of larval development, followed by a decrease in the intermediate stage, and by a subsequent increase in the last larval stage.…”

Section: Discussionmentioning

confidence: 99%

10-hydroxy-2E-decenoic Acid (10HDA) is not a Caste-differentiation Factor in Melipona Scutellaris Stingless Bees

Borges

Batista

Malta

et al. 2020

Preprint

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In bees from genus Melipona, differential feeding is not enough to fully explain female polyphenism. In these bees, there is a hypothesis that in addition to the environmental component (food), a genetic component is also involved caste differentiation regulation. This mechanism has not yet been fully elucidated and may involve epigenetic and metabolic regulation. Here, we analysed the expression of the genes encoding histone deacetylases HDAC1 and HDAC4 and histone acetyltransferase KAT2A in Melipona scutellaris. We also investigated the metabolic profile of larvae and larval food to search for putative queen-fate inducing compounds. Finally, we assessed the effect of the histone deacetylase inhibitor 10-hydroxy-2E-decenoic acid (10HDA) - the major lipid component of royal jelly and hence a putative regulator of honeybee caste differentiation - on Melipona caste differentiation. The hdac1, hdac4 and kat2a transcripts were expressed at all stages, with fluctuations in developmental stages and castes, which may be related to endocrine regulation. We did not identify the putative caste-differentiation factors, geraniol and 10HDA. Also, 10HDA was unable to promote differentiation in queens. Our results suggest that epigenetic and hormonal regulations act synergistically for drive caste differentiation in Melipona and that 10HDA is not a caste-differentiation factor in Melipona scutellaris.

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“…The data were analyzed using analysis of variance, each pair student's t-test. Mean values with the same letter are not significantly different from each other HDAC3 is expressed during all developmental stages, and the highest mRNA levels were detected in one-dayold pupae [36]. We also demonstrated that the depletion of HDAC3 during the pre-pupal stage (72-h old) T. castaneum results in abnormal pupal development.…”

Section: Discussionmentioning

confidence: 48%

Histone deacetylase 3 is required for development and metamorphosis in the red flour beetle, Tribolium castaneum

George

Palli

2020

BMC Genomics

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Background: Hormones are chemical communication signaling molecules released into the body fluids to stimulate target cells of multicellular organisms. We recently showed that histone deacetylase 1 (HDAC1) plays an important role in juvenile hormone (JH) suppression of metamorphosis in the red flour beetle, Tribolium castaneum. Here, we investigated the function of another class I HDAC member, HDAC3, and show that it is required for the normal development of T. castaneum. Results: RNA interference-mediated knockdown of the HDAC3 gene affected development resulting in abnormally folded wings in pupae and adults. JH analog, hydroprene, suppressed the expression of HDAC3 in T. castaneum larvae. The knockdown of HDAC3 during the final instar larval stage resulted in an increase in the expression of genes coding for proteins involved in JH action. Sequencing of RNA isolated from larvae injected with dsRNA targeting malE (E. coli gene, control) or HDAC3 followed by differential gene expression analysis identified 148 and 741 differentially expressed genes based on the P-value < 0.01 and four-fold difference, and the P-value < 0.05 and twofold difference, respectively. Several genes, including those coding for myosin-I heavy chain (Myosin 22), Shaven, and nuclear receptor corepressor 1 were identified as differentially expressed genes in HDAC3 knockdown larvae. An increase in histone H3 acetylation, specifically H3K9, H3K18, and H3K27, was detected in HDAC3 knockdown insects. Conclusion: Overall, these data suggest that HDAC3 affects the acetylation levels of histones and influences the expression of genes coding for proteins involved in the regulation of growth, development, and metamorphosis.

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Molecular characterization of class I histone deacetylases and their expression in response to thermal and oxidative stresses in the red flour beetle, Tribolium castaneum

Cited by 7 publications

References 35 publications

10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees

10-hydroxy-2E-decenoic acid (10HDA) does not promote caste differentiation in Melipona scutellaris stingless bees

10-hydroxy-2E-decenoic Acid (10HDA) is not a Caste-differentiation Factor in Melipona Scutellaris Stingless Bees

Histone deacetylase 3 is required for development and metamorphosis in the red flour beetle, Tribolium castaneum

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