Molecular characterization of clinical and environmental Vibrio parahaemolyticus isolates in Taiwan

Tsai, Shing-en; Jong, Koa-Jen; Tey, Yao Hsien; Yu, Wei-Ting; Chiou, Chien-Shun; Lee, Yeong-Sheng; Wong, Hin-Chung

doi:10.1016/j.ijfoodmicro.2013.04.017

Cited by 28 publications

(27 citation statements)

References 59 publications

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“…PFGE has been used as the “gold standard” method to assess genetic diversity and clonal relatedness among V . parahaemolyticus isolates from different sources [18, 20, 25]. …”

Section: Discussionmentioning

confidence: 99%

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Characterization of clinical Vibrio parahaemolyticus strains in Zhoushan, China, from 2013 to 2014

Wang¹,

Tang²,

et al. 2017

PLoS ONE

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Vibrio parahaemolyticus is recognized as major cause of foodborne illness of global public health concern. This study collected 107 strains of V. parahaemolyticus during active surveillance of diarrheal diseases in hospitals in Zhoushan during 2013 to 2014 and investigated their serotypes, virulence genes (tdh, trh, and orf8), antimicrobial resistance, and genotypes. The dominant serotypes of the 107 clinical strains were O3:K6, O4:K8, and O4:KUT with 87.9% and 3.7% of the strains carrying the virulence genes tdh and trh, respectively. Molecular typing by pulsed-field gel electrophoresis indicated divergence among the clinical strains. Most isolates were sensitive to the common antimicrobial agents used against the Vibrio species except ampicillin. We conclude that continuous surveillance of V. parahaemolyticus in diarrhea patients is a public health priority and is useful for conducting risk assessment of foodborne illnesses caused by V. parahaemolyticus.

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“…PFGE has been used as the “gold standard” method to assess genetic diversity and clonal relatedness among V . parahaemolyticus isolates from different sources [18, 20, 25]. …”

Section: Discussionmentioning

confidence: 99%

“…Clustering was performed using the unweighted pair group method and the Dice correlation coefficient with a position tolerance of 1.5%. Clusters were defined on the basis of a 90% similarity cutoff [18]. …”

Section: Methodsmentioning

confidence: 99%

Characterization of clinical Vibrio parahaemolyticus strains in Zhoushan, China, from 2013 to 2014

Wang¹,

Tang²,

et al. 2017

PLoS ONE

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“…The presence of tdh (42) and ctx (39) in V. parahaemolyticus or V. cholerae isolates, respectively, was determined by PCR methods using specific primers (Table 1). PCR was performed using a GeneAmp PCR System 9700 (Applied Biosystems, Foster City, CA) at 94°C for 3 min, followed by 35 cycles of 94°C for 1 min, …”

Section: Methodsmentioning

confidence: 99%

“…In Taiwan, V. parahaemolyticus is the most prevalent foodbome pathogenic bacteria and is generally associated with the consumption of contaminated seafood (42,43), while cases of V. vulnificus (9) and V. cholerae (38) infection are sporadic.…”

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confidence: 99%

Occurrence of Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio vulnificus in the Aquacultural Environments of Taiwan

Tey

Jong

Fen

et al. 2015

Journal of Food Protection

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The occurrence of Vibrio parahaemolyticus, Vibrio vulnificus, and Vibrio cholerae in a total of 72 samples from six aquaculture ponds for groupers, milk fish, and tilapia in southern Taiwan was examined by the membrane filtration and colony hybridization method. The halophilic V. parahaemolyticus was only recovered in seawater ponds, with a high isolation frequency of 86.1% and a mean density of 2.6 log CFU/g. V. cholerae was found in both the seawater and freshwater ponds but preferentially in freshwater ponds, with a frequency of 72.2% and a mean density of 1.65 log CFU/g. V. vulnificus was identified mainly in seawater ponds, with an isolation frequency of 27.8%. The density of V. parahaemolyticus in seawater ponds was positively related to water temperature (Pearson correlation coefficient, r = 0.555) and negatively related to salinity (r = 2 0.333). The density of V. cholerae in all six ponds was positively related to water temperature (r = 0.342) and negatively related to salinity (r = 2 0.432). Two putatively pathogenic tdh(+) V. parahaemolyticus isolates (1.4% of the samples) and no ctx(+) V. cholerae isolates were identified. The experimental results may facilitate assessments of the risk posed by these pathogenic Vibrio species in Taiwan, where aquaculture provides a large part of the seafood supply.

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“…For children, the elderly, and people with suppressed immune systems, V. parahaemolyticus intestinal infections can be fatal (3). The pathogenicity of V. parahaemolyticus is always associated with the presence of the toxin genes tdh (encoding the thermostable direct hemolysin [TDH]) and its homolog trh (encoding the thermostable direct hemolysin [TDH]-related hemolysin) (4). The U.S. Food and Drug Administration estimates that approximately 4,500 infections occur per year in the United States, and over the past 15 years, outbreaks have been increasing in terms of scale and frequency (3).…”

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confidence: 99%

First Detection of AmpC β-Lactamase bla _CMY-2 on a Conjugative IncA/C Plasmid in a Vibrio parahaemolyticus Isolate of Food Origin

Lin

Chen

et al. 2015

Antimicrob Agents Chemother

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bVibrio parahaemolyticus is an important causative agent of gastroenteritis, with the consumption of contaminated seafood being the major transmission route. Resistance to penicillin is common among V. parahaemolyticus strains, whereas cephalosporin resistance remains rare. In an attempt to assess the current prevalence and characteristics of antibiotic resistance of this pathogen in common food samples, a total of 54 (17% of the total samples) V. parahaemolyticus strains were isolated from 318 meat and seafood samples purchased from supermarkets and wet markets in Shenzhen, China, in 2013. These isolates exhibited high-level resistance to ampicillin, yet they were mostly susceptible to other antimicrobials, except for two that were resistant to extended-spectrum cephalosporins. The ␤-lactamase gene bla PER-1 was detectable in one strain, V. parahaemolyticus V43, which was resistant to both third-and fourth-generation cephalosporins. Compared to other bla PER-1 -positive V. parahaemolyticus strains reported in our previous studies, strain V43 was found to harbor an ϳ200-kb conjugative plasmid carrying genes that were different from the antimicrobial resistance genes reported from the previous studies. The ␤-lactamase gene bla CMY-2 was detectable for the first time in another V. parahaemolyticus isolate, V4, which was resistant to third-generation cephalosporins. This bla CMY-2 gene was shown to be located in an ϳ150-kb IncA/C-type conjugative plasmid with a genetic structure consisting of traB-traV-traA-ISEcp1-bla CMY-2 -blc-sugE-encR-orf1-orf2-orf3-orf4-dsbC-traC, which is identical to that of other IncA/C conjugative plasmids in Enterobacteriaceae, albeit with a different size. These findings indicate that the transmission of extendedspectrum-␤-lactamase (ESBL) and AmpC ␤-lactamase genes via conjugative plasmids can mediate the development of extendedspectrum cephalosporin resistance in V. parahaemolyticus, thereby posing a potential threat to public health. Vibrio parahaemolyticus is a halophilic Gram-negative bacterium and one of the most important seafood-borne pathogens worldwide. It is widely distributed in estuarine-marine environments and seafood products, often causing infections via the consumption of raw or undercooked seafood (1, 2). The most common symptoms of V. parahaemolyticus infections include gastroenteritis, diarrhea, headache, nausea, and vomiting. For children, the elderly, and people with suppressed immune systems, V. parahaemolyticus intestinal infections can be fatal (3). The pathogenicity of V. parahaemolyticus is always associated with the presence of the toxin genes tdh (encoding the thermostable direct hemolysin [TDH]) and its homolog trh (encoding the thermostable direct hemolysin [TDH]-related hemolysin) (4). The U.S. Food and Drug Administration estimates that approximately 4,500 infections occur per year in the United States, and over the past 15 years, outbreaks have been increasing in terms of scale and frequency (3). In recent years, the number of V. parahaemolyticus infecti...

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Molecular characterization of clinical and environmental Vibrio parahaemolyticus isolates in Taiwan

Cited by 28 publications

References 59 publications

Characterization of clinical Vibrio parahaemolyticus strains in Zhoushan, China, from 2013 to 2014

Characterization of clinical Vibrio parahaemolyticus strains in Zhoushan, China, from 2013 to 2014

Occurrence of Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio vulnificus in the Aquacultural Environments of Taiwan

First Detection of AmpC β-Lactamase bla _CMY-2 on a Conjugative IncA/C Plasmid in a Vibrio parahaemolyticus Isolate of Food Origin

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Molecular characterization of clinical and environmental Vibrio parahaemolyticus isolates in Taiwan

Cited by 28 publications

References 59 publications

Characterization of clinical Vibrio parahaemolyticus strains in Zhoushan, China, from 2013 to 2014

Characterization of clinical Vibrio parahaemolyticus strains in Zhoushan, China, from 2013 to 2014

Occurrence of Vibrio parahaemolyticus, Vibrio cholerae, and Vibrio vulnificus in the Aquacultural Environments of Taiwan

First Detection of AmpC β-Lactamase bla CMY-2 on a Conjugative IncA/C Plasmid in a Vibrio parahaemolyticus Isolate of Food Origin

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Product

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First Detection of AmpC β-Lactamase bla _CMY-2 on a Conjugative IncA/C Plasmid in a Vibrio parahaemolyticus Isolate of Food Origin