Molecular characterization of Clostridium perfringens isolated from cattle and sheep carcasses and its antibiotic resistance patterns in Shiraz slaughterhouse, southern Iran

Abstract

________________________________________________________________________________________ HoSSeInzaDeH, S., M. BaHaDorI, M. PoorMontaSerI, M. DeHgHanI, M. FazelI, S. nazIFI: Molecular characterization of Clostridium perfringens isolated from cattle and sheep carcasses and its antibiotic resistance patterns in Shiraz slaughterhouse, southern Iran. Vet. arhiv 88, 581-591, 2018.aBStraCt Clostridium perfringens type A food-borne poisoning is often caused by C. perfringens enterotoxin (CPE) encoded by chromosomal cpe. Contamination of meat with C. perfringens usually leads to food poisoning outbreaks. To find more information regarding the causative agent, we focused on the identification of type A containing cpe and netB genes in cattle and sheep carcasses slaughtered at Shiraz slaughterhouse and investigated the prevalence of antibiotic-resistant plasmid in isolated C. perfringens. 200 specimens were randomly collected by swabbing the whole outer and inner surface of the carcasses, and processed for selective culture on sulfadiazine polymyxin sulphate agar (SPS). The suspected colonies were further identified using species-specific primers as to confirm the presence of the cpa, cpe, netB and tetracycline and enrofloxacin gene resistance patterns. Our results demonstrated that out of 90 and 70 colonies of the positive cultures from cattle and sheep samples, respectively, 40% and 35.7% of the suspected colonies were identified as C. perfringens type A by PCR assay. Moreover, from those type A isolates, only 1 (2.7%) isolate was positive for both cpe and netB genes in the cattle carcasses. The MIC values also showed high tetracycline resistance patterns for cattle (45.8%) and sheep (92.3%) while all of the PCR positive C. perfringens type A isolates were susceptible to enrofloxacin. The high prevalence of C. perfringens in slaughtered animals with a high rate of resistance to tetracycline implies the need for caution in the use of antibiotic in food animals.

The prevalence of antibiotic-resistant Clostridium species in Iran: a meta-analysis

“…Additionally, there is no comprehensive information about the emergence of antibiotic-resistant C. difficile in other countries such as Iran. C. perfringens is another obligate anaerobic Gram-positive organism which is localized in the intestinal tract of humans, animals and insects and also found in the environment such as in soil and water [7]. The bacterium can contaminate wounds and cause soft tissue infections including cellulitis, suppurative myositis and myonecrosis [1,7].…”

“…C. perfringens is another obligate anaerobic Gram-positive organism which is localized in the intestinal tract of humans, animals and insects and also found in the environment such as in soil and water [7]. The bacterium can contaminate wounds and cause soft tissue infections including cellulitis, suppurative myositis and myonecrosis [1,7]. Enterotoxinproducing type A C. perfringens is responsible for gastroenteritis, including food poisoning and necrotizing enteritis after consuming meat products particularly raw meat and poultry [1,7].…”

Teneur en minéraux principaux de la viande cameline en Algérie

“…La teneur en phosphore était de l'ordre de 655 mg / 100 g de MS soit environ 160 mg / 100 g de MF. Ces données sont en accord avec celles de 250-584 mg / 100 g de MS de Kadim et al (2006), et de 162 mg / 100 g de MF de Gheisari et al (2009). Avec des valeurs de 100-200 mg / 100 g de MF, le phosphore est le deuxième élément le plus abondant dans la viande de dromadaire.…”

“…Ces valeurs sont toutefois nettement plus faibles que celles de 105-260 mg / 100 g de MF rapportées par Kadim et al (2006). A titre de comparaison, la teneur en sodium de la viande de bovin est de 51 mg / 100 g de MF (Gheisari et al, 2009 dans ces milieux. La natrémie de cet herbivore est par ailleurs physiologiquement plus élevée que chez les ruminants (Faye et Bengoumi, 2000) et l'hypernatrémie est physiologiquement observée chez l'animal déshydraté (Faye et Bengoumi, 2000).…”

“…Toutefois, Kadim et al (2006) rapportent des teneurs en cendres de 0,76 % chez les chameaux de moins de cinq ans, contre 0,86 % chez les dromadaires ayant cinq ans ou plus. Gheisari et al (2009) rapportent un effet significatif de l'âge sur la teneur en cendres de la viande de chameau, à l'inverse des résultats obtenus par Raiymbek et al (2012). Finalement, il convient de noter que les effets de la race observés dans notre étude doivent être considérés avec beaucoup de prudence étant donné que les animaux de race Targui appartenaient à une seule classe d'âge (cinq ans).…”