SUMMARY:The aim of this study was to determine the resistance patterns of uropathogenic Escherichia coli (UPEC) isolates and to investigate the frequency of several virulence genes, including fimH, papA, hlyD, cnf-1, sitA, and tsh, among various phylogenetic groups of UPEC isolates. A total of 85 E. coli isolates were recovered from urine samples from outpatients with a clinical diagnosis of uncomplicated urinary tract infections. A molecular approach to examine the antimicrobial resistance patterns was employed using PCR and the disc diffusion method. The detected frequencies of the virulence factor genes determined using PCR were: fimH (34.1z), papA (9.4z), hlyD (21.2z), cnf-1 (3.5z), sitA (15.3z), and tsh (27.1z). These results revealed that the isolates were resistant to trimethoprimsulfamethoxazole (SXT) (74.1z), cefotaxime (CTX) (68.2z), and amoxicillin-clavulanic acid (AMC) (94.1z), and they were relatively less resistant to N (56.5z). According to these results, further investigation is needed to determine exactly whether or not SXT, CTX, and AMC are appropriate antibiotics for the treatment of UPEC infections in southern Iran. Although these results demonstrate that fimH is the most frequent virulence gene among UPEC isolates, the high prevalence of isolates that do not encode fimH (75.9z) and the relatively low frequency of isolates that carry other virulence genes require further investigation to clarify the role of the other potential virulence factors in the pathogenesis of these isolates.

A total of 85 Uropathogenic Escherichia coli (UPEC) isolates were screened against ceftiofur, oxacillin, nitrofurantoin and lincospectin using Kirby-Bauer disc diffusion method, following CLSI guidelines. Prevalence of virulent factor genes amongst the isolates was determined by PCR, using gene-specific primers against the different virulent factors. Statistical analysis of the data was performed using SPSS software. The prevalence of traT, ompT, Iss, malX and ibeA genes was 47.1%, 38.8%, 20%, 16.5% and 9.4%, respectively. The most prevalent gene in group A and D was traT, whilst in group B2 was Iss. The highest resistance has been shown against oxacillin (98.8%), followed by ceftiofur (77.6%), whilst resistance to lincospectin (2.4%) and nitrofurantoin (12.9%) had the lowest frequencies. Multidrug resistance was shown in 82.35% of the isolates, whilst this study recommend lincospectin and nitrofurantoin as choice drugs for treatment, but more investigation of the bacterial pathogenicity associated with urinary tract infection (UTI) may contribute to a better medical intervention.

It is common knowledge that fecal microbiota is a primary source of Escherichia coli causing urinary tract infections (UTIs) via the fecal‐perineal‐urethral route. But, it is still unknown whether E. coli UTI is mainly caused by dominant fecal E. coli isolates (prevalence hypothesis) or the isolates that possess more virulence factors (special pathogenicity hypothesis). In the present study, the urine E. coli isolates of 30 women with UTI were compared with the fecal E. coli isolates of the same patients and healthy control individuals according to the phylogenetic group, virulence genotype, and antibiotic susceptibility pattern. The genetic relatedness of the isolates was specified and compared by pulsed‐field gel electrophoresis (PFGE). PFGE analysis showed that most patients (73.3%) had distinct urine isolates which were not similar to any of their fecal isolates. Based on the phylogenetic analysis, most of the urine and fecal isolates of healthy women were assigned to phylogenetic group B2, followed by D. The distribution of phylogenetic groups was significantly different between the urine and the fecal isolates of patients ( p < 0.05). The prevalence of fimH and ompT among urine isolates was significantly more than that among fecal isolates. The level of multidrug resistance was higher among urine isolates. Although more in‐depth researches are required, the present study could be supported by pathogenicity hypothesis. Furthermore, concerning the antibiotic resistance pattern among uropathogenic E. coli should be highly considered.

Sulfonyl fluorides have emerged as powerful "click" electrophiles to access sulfonylated derivatives. Yet, they are relatively inert towards C À C bond forming transformations, notably under transition-metal catalysis. Here, we describe conditions under which aryl sulfonyl fluorides act as electrophiles for the Pd-catalyzed Suzuki-Miyaura cross-coupling. This desulfonative cross-coupling occurs selectively in the absence of base and, unusually, even in the presence of strong acids. Divergent one-step syntheses of two analogues of bioactive compounds showcase the expanded reactivity of sulfonyl fluorides to encompass both SÀNu and CÀC bond formation. Mechanistic experiments and DFT calculations suggest oxidative addition occurs at the CÀS bond followed by desulfonation to form a Pd-F intermediate that facilitates transmetalation.

________________________________________________________________________________________ HoSSeInzaDeH, S., M. BaHaDorI, M. PoorMontaSerI, M. DeHgHanI, M. FazelI, S. nazIFI: Molecular characterization of Clostridium perfringens isolated from cattle and sheep carcasses and its antibiotic resistance patterns in Shiraz slaughterhouse, southern Iran. Vet. arhiv 88, 581-591, 2018.aBStraCt Clostridium perfringens type A food-borne poisoning is often caused by C. perfringens enterotoxin (CPE) encoded by chromosomal cpe. Contamination of meat with C. perfringens usually leads to food poisoning outbreaks. To find more information regarding the causative agent, we focused on the identification of type A containing cpe and netB genes in cattle and sheep carcasses slaughtered at Shiraz slaughterhouse and investigated the prevalence of antibiotic-resistant plasmid in isolated C. perfringens. 200 specimens were randomly collected by swabbing the whole outer and inner surface of the carcasses, and processed for selective culture on sulfadiazine polymyxin sulphate agar (SPS). The suspected colonies were further identified using species-specific primers as to confirm the presence of the cpa, cpe, netB and tetracycline and enrofloxacin gene resistance patterns. Our results demonstrated that out of 90 and 70 colonies of the positive cultures from cattle and sheep samples, respectively, 40% and 35.7% of the suspected colonies were identified as C. perfringens type A by PCR assay. Moreover, from those type A isolates, only 1 (2.7%) isolate was positive for both cpe and netB genes in the cattle carcasses. The MIC values also showed high tetracycline resistance patterns for cattle (45.8%) and sheep (92.3%) while all of the PCR positive C. perfringens type A isolates were susceptible to enrofloxacin. The high prevalence of C. perfringens in slaughtered animals with a high rate of resistance to tetracycline implies the need for caution in the use of antibiotic in food animals.