Molecular characterization of conditionally immortalized cell lines derived from mouse early embryonic inner ear

Germiller, John A.; Smiley, Elizabeth; Ellis, Amanda D.; Hoff, Jessica; Deshmukh, Ian; Allen, Susan J.; Barald, Kate F.

doi:10.1002/dvdy.20186

Cited by 19 publications

(22 citation statements)

References 64 publications

(86 reference statements)

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“…The present study tested whether conditioned medium from conditionally immortalized otocyst cell lines derived from the transgenic Immortomouse (Barald et al 1997;Bianchi and Barald 1998;Thompson et al 2003;Germiller et al 2004) provides a source of ODF-like activity. The cells used in the present study were derived from embryonic day (E) 9.5 otocysts as previously described (Barald et al 1997;Germiller et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…The cells used in the present study were derived from embryonic day (E) 9.5 otocysts as previously described (Barald et al 1997;Germiller et al 2004). A total of 307 IMO cell lines were cloned from these otocysts and several have previously been characterized (Thompson et al 2003;Germiller et al 2004). Eight IMO cell lines were identified as positive for hair cell markers such as myosin VIIa, as well as supporting cell marker Hes 1 (Germiller et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…A total of 307 IMO cell lines were cloned from these otocysts and several have previously been characterized (Thompson et al 2003;Germiller et al 2004). Eight IMO cell lines were identified as positive for hair cell markers such as myosin VIIa, as well as supporting cell marker Hes 1 (Germiller et al 2004). However, individual cells in these cloned lines were found to express HC and SC markers simultaneously in culture.…”

Section: Introductionmentioning

confidence: 99%

“…However, individual cells in these cloned lines were found to express HC and SC markers simultaneously in culture. These cell lines, which are derived from the very early otocyst, appear to represent pluripotent precursor cell lines that are common precursors to HC and SC, and possibly SAG neurons (Germiller et al 2004).…”

Section: Introductionmentioning

confidence: 99%

“…In the present study, conditioned medium was screened from five IMO cell lines that express hair cell and supporting cell markers (Germiller et al 2004). Four of the five lines tested were able to promote neurite outgrowth from SAG explants.…”

Section: Introductionmentioning

confidence: 99%

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Immortalized Mouse Inner Ear Cell Lines Demonstrate a Role for Chemokines in Promoting the Growth of Developing Statoacoustic Ganglion Neurons

Bianchi

Daruwalla

Roth

et al. 2005

JARO

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The target-derived factors necessary for promoting initial outgrowth from the statoacoustic ganglion (SAG) to the inner ear have not been fully characterized. In the present study, conditioned medium from embryonic Immortomouse inner ear cell lines that maintain many characteristics of developing inner ear sensory epithelia were screened for neurite-promoting activity. Conditioned medium found to be positive for promoting SAG neurite outgrowth and neuronal survival was then tested for the presence of chemokines, molecules that have not previously been investigated for promoting SAG outgrowth. One candidate molecule, monocyte chemotactic protein 1 (MCP-1), was detected in the conditioned medium and subsequently localized to mouse hair cells by immunocytochemistry. In vitro studies demonstrated that function-blocking MCP-1 antibodies decreased the amount of SAG neurite outgrowth induced by the conditioned medium and that subsequent addition of MCP-1 protein was able to promote outgrowth when added to the antibodytreated conditioned medium. The use of the Immortomouse cell lines proved valuable in identifying this candidate cofactor that promotes outgrowth of earlystage SAG nerve fibers and is expressed in embryonic hair cells.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Immortalized Mouse Inner Ear Cell Lines Demonstrate a Role for Chemokines in Promoting the Growth of Developing Statoacoustic Ganglion Neurons

Bianchi

Daruwalla

Roth

et al. 2005

JARO

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Concomitant differentiation of a population of mouse embryonic stem cells into neuron‐like cells and schwann cell–like cells in a slow‐flow microfluidic device

Ramamurthy

White

Park

et al. 2016

Developmental Dynamics

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Background To send meaningful information to the brain, an inner ear cochlear implant (CI) must become closely coupled to as large and healthy a population of remaining Spiral Ganglion Neurons (SGN) as possible. Inner ear gangliogenesis depends on macrophage migration inhibitory factor (MIF), a directionally attractant neurotrophic cytokine made by both Schwann and supporting cells (Bank et al., 2012). MIF-induced mouse embryonic stem cell (mESC)-derived “neurons” could potentially substitute for lost or damaged SGN. mESC-derived “Schwann cells” produce MIF as do all Schwann cells (Huang et al., 2002; Roth et al., 2007, 2008) and could attract SGN to “ cell coated” implant. Results Neuron- and Schwann cell-like cells were produced from a common population of mESC in an ultra-slow flow microfluidic device. As the populations interacted; “neurons” grew over the “Schwann cell” lawn and early events in myelination were documented. Blocking MIF on the Schwann cell side greatly reduced directional neurite outgrowth. MIF-expressing “Schwann cells” were used to “coat” a CI: mouse SGN and MIF-induced “neurons” grew directionally to the CI and to a wild type but not MIF-knock out Organ of Corti explant. Conclusions Two novel stem cell-based approaches for treating the problem of sensorineural hearing loss are described.

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Overview: Regeneration and Repair

Salvi¹

Hair Cell Regeneration, Repair, and Protection

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Molecular characterization of conditionally immortalized cell lines derived from mouse early embryonic inner ear

Cited by 19 publications

References 64 publications

Immortalized Mouse Inner Ear Cell Lines Demonstrate a Role for Chemokines in Promoting the Growth of Developing Statoacoustic Ganglion Neurons

Immortalized Mouse Inner Ear Cell Lines Demonstrate a Role for Chemokines in Promoting the Growth of Developing Statoacoustic Ganglion Neurons

Concomitant differentiation of a population of mouse embryonic stem cells into neuron‐like cells and schwann cell–like cells in a slow‐flow microfluidic device

Overview: Regeneration and Repair

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