Molecular characterization of cucumber mosaic virus infecting <i>Echinacea purpurea</i> in Ukraine

Mіshchеnkо, Lidiya; Dunich, Alina; Dashchenko, Anna; Bondus, R.O.

doi:10.1080/03235408.2021.1899378

Cited by 5 publications

(3 citation statements)

References 37 publications

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“…Phylogenetic analysis of the 593 bp coat protein gene of CMV demonstrated that the isolate studied was closely related to the African isolates in subgroup Ib and other global isolates. Mishchenko et al [42] deposited the 443 bp CP gene sequence of the CMV isolate P-EP-Ukr-19, which infects Echinacea purpurea (cone ower) plants in Ukraine, in the NCBI GenBank with the accession no. MT978189.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity of CMV and ToMV Isolates in Tomato and Pepper Production Areas in Hakkari, Turkey

AKDURA

Kiliç

2022

Preprint

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Background Tomato (Lycopersicum esculentum Mill.) and pepper (Capsicum anuum L.) are the host species for the Cucumber mosaic virus (CMV) and Tomato mosaic virus (ToMV). This article aims to report the detection of serolocigally and molecularly and identification of genetic diversity these viruses in Hakkari province, Turkey.Methods and results A total of 184 leaf samples were collected from tomato and pepper plants that showed virus-like symptoms. Sample collection was carried out in Hakkari province in October, 2020. CMV and ToMV factors were investigated by Double Antibody Sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA). DAS-ELISA test showed that 106 (57.60%) of the samples were infected with one or more viruses. For phylogenetic analyses, 10 samples which were positive for CMV and ToMV were tested using Reverse Transcription Polymerase Chain Reaction (RT-PCR) method. Coat protein (CP) and replicase genes of Hakkari CMV and ToMV isolates were amplified by RT-PCR using specific primers. The resulting DNA sequences were deposited in NCBI GenBank (Accession Nos: OM286731, OM286732, OM286733, OM286734, OM418629). When the replicase and coat protein gene sequences of four ToMV isolates and one CMV isolate were compared with the isolates in GenBank, a nucleotide homology of 98.1-99.6% and 91.7-99.4% were found, respectively. Phylogenetic analysis revealed a strong similarity between Hakkari ToMV and CMV isolates and other Turkish isolates. Consistent with the molecular studies conducted in Turkey on CMV and ToMV, the isolates detected in this study also clustered in subgroup Ia. Conclusions With this study, CMV and ToMV factors were serologically and molecularly detected for the first time in Hakkari and the first nation-wide report for ToMV replicase sequences in infected tomato and peppers were submitted.

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Section: Discussionmentioning

confidence: 99%

Genetic Diversity of CMV and ToMV Isolates in Tomato and Pepper Production Areas in Hakkari, Turkey

AKDURA

Kiliç

2022

Preprint

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“…Visually healthy gladioli did not contain CMV antigens. In 2020, corms from healthy gladioli were planted in field conditions in the Poltava region, where the circulation of cucumber mosaic virus of IB subgroup was previously revealed on other plants (Mishchenko et al, 2021). In gladioli planted in 2020, symptoms of stripes on the leaves were observed (Fig.…”

Section: Resultsmentioning

confidence: 94%

“…Fifteen samples of gladiolus with both virus-specific symptoms and visually healthy plants were selected. Healthy plants that were confirmed by ELISA and RT-PCR have been planted next year in field conditions in the Poltava region (central Ukraine) in the agrocenosis where CMV circulation was revealed (Mishchenko et al, 2021).…”

Section: Samples Collection and Visual Diagnosticsmentioning

confidence: 87%

Phylogenetic Analysis of Two Ukrainian Isolates of Cucumber Mosaic Virus From Gladioli Grown Under Different Agroecological Conditions

Sovinska¹,

Dunich²,

Mіshchеnkо³

2021

AgricultForest

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Cucumber mosaic virus (CMV) is one of the most widespread and harmful viruses infecting gladiolus plants worldwide. The aim of the study was to perform a phylogenetic analysis of two CMV isolates from gladioli grown in different regions of Ukraine. For the first time, 443 nt sequences of the capsid protein (CP) gene of gladiolus isolates CMV-Gl-Skv-20 (MW847710) and CMV-Gl-SkvP-20 (MW847714) from Kyiv and Poltava regions, respectively, were submitted to the NCBI GeneBank. Phylogenetic analysis showed that isolates clustered with different phylogenetic subgroups. CMV-Gl-Skv-20 belongs to subgroup IA, and has nucleotide (nt) sequence identity 81.9%-99.27% and amino acid (aa) identity 82.3%-97.6% with isolates from this group. The highest identity of the CMV-Gl-Skv-20 was found to be with Turkish CMVs from Rapistrum rugosum TUR83, TUR86 and Brassica TUR4 (98.8%-99.3% nt and 97,6% aa), as well as with Australian isolates Ny and 207 from tomato, and banana isolate Cameroon (98.9%-99% nt and 97,6% aa). CMV-Gl-SkvP-20 belongs to subgroup IB and shares 95.8%-100% nt and 96%-100% aa identity with the members of this subgroup. CMV-Gl-SkvP-20 has the highest identity with Ukrainian isolates from cucumber Ukr-1409 and Echinacea P-EP-Ukr-19 (99.5%-100% nt and 99.2-100% aa), Chinese pumpkin isolates ZBR, WHR, isolate lu-17-14 from sweet potato and SXCH from Bupleurum sp. (98.3-99% nt and 98.4%-99.2% aa), as well as with gladiolus South Korean isolate ABI (98.3% nt and 97.7% aa). The results support the fact that gladioli are affected by CMV not only through the corms, but by vector insects circulating in growing areas.

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First report of сucumber mosaic virus infecting Arctium lappa in Ukraine

et al. 2022

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Molecular characterization of cucumber mosaic virus infecting Echinacea purpurea in Ukraine

Cited by 5 publications

References 37 publications

Genetic Diversity of CMV and ToMV Isolates in Tomato and Pepper Production Areas in Hakkari, Turkey

Genetic Diversity of CMV and ToMV Isolates in Tomato and Pepper Production Areas in Hakkari, Turkey

Phylogenetic Analysis of Two Ukrainian Isolates of Cucumber Mosaic Virus From Gladioli Grown Under Different Agroecological Conditions

First report of сucumber mosaic virus infecting Arctium lappa in Ukraine

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