Molecular Characterization of Cyst Nematode Species (<i>Heterodera</i> spp.) from the Mediterranean Basin using RFLPs and Sequences of ITS‐rDNA

Madani, Mehrdad; Vovlas, N.; Castillo, Pablo; Subbotin, Sergei A.; Moens, Maurice

doi:10.1111/j.1439-0434.2004.00835.x

Cited by 56 publications

(43 citation statements)

References 16 publications

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“…The H. latipons populations in this study consisted of 2 subgroups. Similarly, differences in ITS sequences were previously reported for other H. latipons populations (Ferris et al, 1999;Madani et al, 2004).…”

Section: Discussionmentioning

confidence: 50%

“…In agreement with Madani et al (2004), Cryphodera brinkmani (AF274418) and Meloidodera alni (AF274419) were also incorporated in the phylogenetic analysis. For the phylogenetic analysis, H. latipons (Turkey-JX024184; Syria-JX024179, JX024182; Iran-HM560790; Morocco-…”

Section: Phylogenetic Analysismentioning

confidence: 68%

“…The ribosomal genes are highly conserved, allowing for the construction of polymerase chain reaction (PCR) primers. However, the ITS regions separating the ribosomal genes are less conserved, which allows for the detection of polymorphisms (Madani et al, 2004;Kumar et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Morphological and molecular identification of cereal cyst nematodes from the eastern Mediterranean region of Turkey

İmren¹,

Waeyenberge²,

Viaene³

et al. 2015

Turk J Agric For

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The morphological and molecular characteristics of 41 populations of cereal cyst nematodes (Heterodera avenae group) collected in Adana, Osmaniye, Kahramanmaraş, Hatay, Gaziantep, and Kilis provinces in the eastern Mediterranean region of Turkey were studied. The morphological characters and morphometric features of second-stage juveniles and cysts showed the presence of 3 Heterodera species: H. avenae, H. filipjevi, and H. latipons. All morphological values of these distinct populations were very similar to those previously described for these species. Genetic variation was observed among the identified cyst nematode species H. avenae, H. filipjevi, and H. latipons. Intraspecific polymorphism was observed within H. avenae and H. latipons but not in H. filipjevi populations. Molecular analysis using ITS regions of rDNA confirmed the identities of the 3 Heterodera species. According to our results, 75% of isolates were identified as H. avenae, 15% as H. latipons, and 10% as H. filipjevi.

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Section: Discussionmentioning

confidence: 50%

Section: Phylogenetic Analysismentioning

confidence: 68%

See 1 more Smart Citation

Morphological and molecular identification of cereal cyst nematodes from the eastern Mediterranean region of Turkey

İmren¹,

Waeyenberge²,

Viaene³

et al. 2015

Turk J Agric For

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show abstract

“…The species of Heterodera are classified in several morphologically defined groups: the H. avenae, goettingiana, humuli, schachtii, cyperi, and sacchari groups (Baldwin and Mundo-Ocampo 1991;Subbotin et al 2001). Phylogenetic relationships among groups and species have been studied intensively using the ITS-rRNA sequences (Ferris et al 1993;Sabo et al 2001;Subbotin et al 2001;Tanha Maafi et al 2003;Madani et al 2004). However, none of these studies has considered structural components of the ITS-rRNA molecule.…”

Section: Introductionmentioning

confidence: 99%

ITS2 secondary structure and phylogeny of cyst-forming nematodes of the genus Heterodera (Tylenchida: Heteroderidae)

Overstreet

Subbotin

2008

Organisms Diversity & Evolution

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The internal transcribed spacer 2 (ITS2) of the ribosomal RNA gene is a double-edged tool for eukaryote evolutionary comparison. In this paper, we re-evaluate the putative ITS2 secondary structures proposed for 29 species of cyst-forming nematodes in the genus Heterodera and present optimized variants. The models are organized in four main helices emerging from a central core. Helix III is always the longest and has two branches exhibited as a Y-shaped structure near the apex. In most species, helices III and IV have merged to form a segment of the central core. Two conservative motifs, GAUCR and UURC, were found in helices II and III, respectively. Using the MARNA program taking into consideration both the primary sequence and the secondary structure, we generated an optimal alignment for Heterodera sequences. The alignment was analyzed by Bayesian inference under a general-time-reversible model and the complex models, and by a maximum parsimony approach using original data and sequence data converted according to secondary-structure information. The consensus ITS2 secondary structure model for Heteroderidae can serve as a useful guide for reconstruction of optimal alignment and can be used as an additional source of data incorporating structural parameters of molecules for the study of nematode phylogeny. Application of the secondary ITS2 structure data allows a more resolved and realistic picture of relationships within Heteroderidae.

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“…Comparative analysis of coding and noncoding regions of ribosomal DNA has become a popular tool for construction of phy-logenetic trees of many organisms including nematodes. Phylogenetic analysis based on the sequences of ITS region of rDNA and D2-D3 expansion region of the large subunit (LSU) rRNA gene were used to examine evolutionary relationships among many group of nematodes including the genus Bursaphelenchus (Ferris et al, 1993;Hoyer et al, 1998;Iwahori et al, 1998;Maafi et al, 2003;Zheng et al, 2003;Madani et al, 2004;Subbotin and Sturhan, 2004;Burgermeister et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

First record of Bursaphelenchus rainulfi on pine trees from eastern China and its phylogenetic relationship with intro-genus species

Jiang

Zheng

2007

J. Zhejiang Univ. - Sci. B

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Abstract:Bursaphelenchus rainulfi isolated from dead pine trees in Zhejiang, China, is described and illustrated. It also provided some molecular characters of the Chinese population, including the PCR-RFLP and sequences of ITS region and D2-D3 expansion region of the large subunit (LSU) rRNA gene. Both the morphological characters and ITS-RFLP patterns match with the original description. The phylogenetic trees based on the 13 sequences of D2-D3 expansion region of the LSU rRNA gene and ITS region of Bursaphelenchus species were constructed, respectively, with the results showing the similar clades. The phylogenetic relationship based on the molecular data is similar to that with morphological characters. This is the first report of the species on pine wood in eastern China.

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Molecular Characterization of Cyst Nematode Species (Heterodera spp.) from the Mediterranean Basin using RFLPs and Sequences of ITS‐rDNA

Cited by 56 publications

References 16 publications

Morphological and molecular identification of cereal cyst nematodes from the eastern Mediterranean region of Turkey

Morphological and molecular identification of cereal cyst nematodes from the eastern Mediterranean region of Turkey

ITS2 secondary structure and phylogeny of cyst-forming nematodes of the genus Heterodera (Tylenchida: Heteroderidae)

First record of Bursaphelenchus rainulfi on pine trees from eastern China and its phylogenetic relationship with intro-genus species

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