Molecular characterization of Ditylenchus dipsaci on Onion in Turkey

Abstract

Ditylenchus dipsaci is a species complex including diploid and polyploid individuals. The onion race of D. dipsaci is a sensu stricto group and has a wide range of host spectrum. Identification of the D. dipsaci onion race is difficult using morphological and morphometrical methods. Species specific primers are mostly used in molecular approaches for identification of D. dipsaci populations. Fifty one morphologically selected Ditylenchus spp. populations from onion production areas in Turkey were subjected to molecular identification using four D. dipsaci species specific primer sets (PF1-PR1, PF2-PR2, DdpS1-rDNA2, DitNF1-rDNA2, H05-H06) targeting 5.8S and 18S rDNA, ITS1 and flanking ITS regions. Thirty nine percent of the nematode samples were positive with four primers tested, while four of the nematode samples gave specific bands with H05-H06 primers. Ditylenchus dipsaci sensu stricto was identified with specific primer sets in Adana,

Susceptibility of different plant species to two populations of Ditylenchus dipsaci Kühn, 1857 (Tylenchida: Anguinidae) from Turkey

“…Nematodes were identified morphologically and with species specific PCR technique using the primers of PF1-PR1, PF2-PR2, DdpS1-rDNA2, DİTNF1-rDNA2, H05-H06, DipU F-DipU R and 18S-26S (Subbotin et al, 2005;Esquibet et al, 2003;Marek et al, 2010). Isolation of DNA and PCR conditions were as same as described by Yavuzaslanoglu et al (2018). Morphological measurements were made according to literature for this nematode (Öztürk, 1990;Sturhan & Brzeski, 1991;Kepenekçi, 1999;Mennan, 2001;Chizhov et al, 2010).…”

Molecular characterization of the Pratylenchus vulnus populations on cereals in Turkey

“…Total genomic DNA was extracted from five individual nematodes in 30 µl extraction buffer as described by Yavuzaslanoglu et al (2018) . The sample was placed at −20°C for 1 hr and then incubated at 65°C for 1 hr.…”

The interaction of the mycorrhizae of the fungus Rhizophagus irregularis (Walker & Schüßler, 2010) (Glomerales: Glomeraceae) and the stem and bulb nematode (Ditylenchus dipsaci Kühn, 1857) (Tylenchida: Anguinidae) on the onion plant (Allium cepa L.) (Asparagales: Amaryllidaceae)

“…The identification of the stem and bulb nematode was performed according to the methodology of Yavuzaslanoglu et al (2018). It was identified as Ditylenchus dipsaci by obtaining bands at 327, 396, 967, 256, 325 and 245 bp using the species specific primers, PF1/PR1, PF2/PR2, 18S/26S, Dip1R/ DipUF, Dit2F/Dit2R and Dit5F/Dit5R, respectively.…”