Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal

Fernandes, Rúben; Amador, Paula; Oliveira, Carla; Prudêncio, Cristina

doi:10.1155/2014/782897

Cited by 48 publications

(33 citation statements)

References 37 publications

(43 reference statements)

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“…These results are based on actual estimates of incidence rates, not the proportion of ESBL among Enterobacteriacae, which is a strength of this study. Few other studies, notably in the United States in 2012 and Portugal in 2010, have compared the geographical distribution of the incidence of ESBL-E infection, showing substantial differences according to the species, with predominance of E. coli and K. pneumoniae [28,29], as in our study.…”

Section: Discussionsupporting

confidence: 65%

Ongoing increasing temporal and geographical trends of the incidence of extended-spectrum beta-lactamase-producing Enterobacteriaceae infections in France, 2009 to 2013

Arnaud¹,

Maugat

Jarlier

et al. 2015

Eurosurveillance

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Citation style for this article:Arnaud I, Maugat S, Jarlier V, Astagneau P. Ongoing increasing temporal and geographical trends of the incidence of extended-spectrum beta-lactamase-producing Enterobacteriaceae infections in France, 2009. Euro Surveill. 201520(36) E xtended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) are a major focus of multidrug-resistant organisms (MRO) surveillance programmes in France. To describe the temporal and geographical trends of these pathogens, we conducted an epidemiological study based on data extracted from the nationwide MRO surveillance network from 2009 to 2013. During this time, the incidence of ESBL-E infections in French hospitals increased by 73%, from 0.35 to 0.60 per 1,000 patient days (PD) (p < 0.001) and ESBL-E bacteraemia by 77%, from 0.03 to 0.05 per 1,000 PD (p < 0.001). The incidence of ESBL-E infections was higher in intensive-care units (1.62 to 2.44 per 1,000 PD (p < 0.001)) than in recovery and long-term care facilities (0.20 to 0.31 per 1,000 PD (p < 0.001)). Escherichia coli was the most frequent extended-spectrum betalactamase-producing (ESBL) pathogen, representing 59% (26,238/44,425) of all ESBL isolates, followed by Klebsiella pneumoniae (20%; 8,856/44,425) in 2013. The most frequent infection was urinary tract infection, for all species. The incidence of ESBL-E varied by region but showed an upward trend overall. Reinforcement of control measures for halting the spread of such MRO is crucial.

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Section: Discussionsupporting

confidence: 65%

Ongoing increasing temporal and geographical trends of the incidence of extended-spectrum beta-lactamase-producing Enterobacteriaceae infections in France, 2009 to 2013

Arnaud¹,

Maugat

Jarlier

et al. 2015

Eurosurveillance

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“…SHV-1 is a species specific beta-lactamase gene in Klebsiella pneumoniae that is chromosomally encoded (Babini and Livermore, 2000). Whereas, TEM-1 are plasmid-coded enzyme variants with identical substrate profile (Chang et al, 2001) Plasmid-coded SHV-1 lactamases are widely distributed in Enterobacteriaceae of clinical origin (Spanu et al, 2002;Fernandes et al, 2014). In this study, 77.8 % of the isolates carrying a blaSHV gene were identified as Klebsiella.…”

Section: Discussionmentioning

confidence: 70%

Antibiotic resistance and virulence genes in coliform water isolates

Stange

Sidhu

Tiehm

et al. 2016

International Journal of Hygiene and Environmental Health

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Please cite this article as: Stange, C., Sidhu, J.P.S., Tiehm, A., Toze, S., Antibiotic resistance and virulence genes in coliform water isolates.International Journal of Hygiene and Environmental Health http://dx.doi.org/10. 1016/j.ijheh.2016.07.015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. 1Antibiotic resistance and virulence genes in coliform water isolates Graphical abstract2 ABSTRACTWidespread fecal pollution of surface water may present a major health risk and a significant pathway for dissemination of antibiotic resistance bacteria. The River Rhine is one of the longest and most important rivers in Europe and an important raw water source for drinking water production. A total of 100 coliform isolates obtained from River Rhine (Germany) were examined for their susceptibility to seven antimicrobial agents. Resistances against amoxicillin, trimethoprim/sulfamethoxazole and tetracycline were detected in 48 %, 11 % and 9 % of isolates respectively. The antibiotic resistance could be traced back to the resistance genes blaTEM, blaSHV, ampC, sul1, sul2, dfrA1, tet(A) and tet (B). Whereby, the ampC gene represents a special case, because its presence is not inevitably linked to a phenotypic antibiotic resistance. Multiple antibiotics resistance was often accompanied by the occurrence of class 1 or 2 integrons. E. coli isolates belonging to phylogenetic groups A and B1(commensal) were more predominant (57 %) compared to B2 and D groups (43 %) which are known to carry virulent genes. Additionally, six E. coli virulence genes were also detected. However, the prevalence of virulence genes in the E. coli isolates was low (not exceeding 4.3 % per gene) and no diarrheagenic E. coli pathotypes were detected. This study demonstrates that surface water is an important reservoir of ARGs for a number of antibiotic classes such as sulfonamide, trimethoprim, betalactam-antibiotics and tetracycline. The occurrence of antibiotic resistance in coliform bacteria isolated from River Rhine provides evidence for the need to develop management strategies to limit the spread of antibiotic resistant bacteria in aquatic environment.

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“…Most of the ESBLs are members of the TEM and SHV β-lactamase families, whereas other groups for example CTX-M, OXA, and PER β-lactamase have been discussed more recently (1). ESBLs are transferable, and they contain a number of mutations that allow them to hydrolyze expanded-spectrum β-lactam antibiotics, such as penicillin; first-, second-, and third generation cephalosporins; and aztreonam (2-4). …”

Section: Introductionmentioning

confidence: 99%

Isolation, Antimicrobial Susceptibility Profile and Detection of Sul1, blaTEM, and blaSHV in Amoxicillin-Clavulanate-Resistant Bacteria Isolated From Retail Sausages in Kampar, Malaysia

Tew

She

Chew

2016

Jundishapur J Microbiol

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BackgroundDue to the overuse of antibiotics in livestock as a growth-promoting agent, the emergence of multi-antibiotic resistant bacteria is becoming a concern.ObjectivesIn this study, we aimed to detect the presence and discover the molecular determinants of foodborne bacteria in retail sausages resistant towards the antibacterial agent amoxicillin-clavulanate.MethodsTwo grams of sausages were chopped into small pieces and transferred into sterile Luria-Bertani (LB) enrichment broths overnight before they were plated on MacConkey agar petri dishes. The bacteria isolated were then screened for amoxicillin-clavulanate resistance, and an antimicrobial susceptibility test of each isolate was performed by using the disc diffusion method. Double synergy and phenotypic tests were carried out to detect the presence of extended spectrum β-lactamase (ESBL). API 20E kit was used to identify the Enterobacteriaceae. All isolates were further examined by polymerase chain reaction (PCR) for resistant genes blaOXA-1, blaOXA-10, plasmid-mediated AmpC (blaCMY and blaDHA), and the chromosome-mediated AmpC, Sul1, blaTEM, and blaSHV genes.ResultsA total of 18 amoxicillin-clavulanate resistant isolates were obtained from seven different types of retail sausages. Only half of them were identified as Enterobacteriaceae, but none were ESBL-producers. All the 18 isolated strains demonstrated resistance towards amoxicillin-clavulanate, penicillin and oxacillin (100%), cefotaxime (71.4%), cefpodoxime (66.7%), and ampicillin (83.3%). blaTEM was the most frequently detected β-lactamase gene. Both plasmid- and chromosomal-bound blaTEM genes were detected in all of the isolated Enterobacteriaceae. blaSHV and Sul1 accounted for 22.2% and 11.1% of the amoxicillin-clavulanate resistant isolates, respectively, whereas blaAMPC, blaCMY, blaDHA, blaOXA-1, and blaOXA-10 were not found in any of the isolates. The only one ESBL-producing bacteria detected in this study was Chryseobacterium meningosepticum, which harbored the blaTEM gene.ConclusionsThe multidrug resistant bacteria that carry antibiotic resistant genes from retail sausages may increase the risk of transmission to humans via the consumption of contaminated sausages. Stricter measures must be taken to address the use of antibiotics in animal agriculture and to consider their potential impact on human health.

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Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal

Cited by 48 publications

References 37 publications

Ongoing increasing temporal and geographical trends of the incidence of extended-spectrum beta-lactamase-producing Enterobacteriaceae infections in France, 2009 to 2013

Ongoing increasing temporal and geographical trends of the incidence of extended-spectrum beta-lactamase-producing Enterobacteriaceae infections in France, 2009 to 2013

Antibiotic resistance and virulence genes in coliform water isolates

Isolation, Antimicrobial Susceptibility Profile and Detection of Sul1, blaTEM, and blaSHV in Amoxicillin-Clavulanate-Resistant Bacteria Isolated From Retail Sausages in Kampar, Malaysia

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