Molecular Characterization of Fusarium oxysporum f. melongenae by ISSR and RAPD Markers on Eggplant

Baysal, Ömür; Siragusa, Maria Gabriella; Gümrükcü, Emine; Zengin, Sinan; Carimi, Francesco; Sajeva, Maurizio; Silva, Jaime A. Teixeira da

doi:10.1007/s10528-010-9336-1

Cited by 57 publications

(36 citation statements)

References 24 publications

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“…2) the strains could be classifi ed into two species: F. verticillioides and F. musae. All strains from Africa belong to F. verticillioides (FVB 8-13) whereas the strains from the Neotropical countries proved to be F. musae 14,18,19).…”

Section: Resultsmentioning

confidence: 99%

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Occurrence of Fusarium verticillioides and Fusarium musae on banana fruits marketed in Hungary

Molnár

Bartók²,

Szécsi

2015

Acta Microbiologica et Immunologica Hungarica

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Fusarium strains were isolated from rotten banana fruit imported into Hungary from some African and some Neotropical countries. The strains were identified using morphological features, 2-benzoxazolinone tolerance, translation elongation factor (EF-1α) sequences and inter simple sequence repeat (ISSR) analysis. All strains from Africa proved to be F. verticillioides whereas the strains from the Neotropics are Fusarium musae. According to the PCR proof and the fumonisin toxin measurement F. musae strains cannot produce any fumonisins (FB1–4).

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Section: Resultsmentioning

confidence: 99%

“…All ISSR PCR amplifi cations were done in a fi nal volume of 25 μl with the following components: DreamTaq Green PCR Master Mix 2× (Fermentas) 12.5 μl, 30 ng of template DNA, 0.6 μM primer and water. PCR parameters were according to Baysal et al [18]. Annealing temperatures were fi tted to the ISSR primers (Table II).…”

Section: Methodsmentioning

confidence: 99%

Occurrence of Fusarium verticillioides and Fusarium musae on banana fruits marketed in Hungary

Molnár

Bartók²,

Szécsi

2015

Acta Microbiologica et Immunologica Hungarica

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“…Although several generations of DNA markers have been developed and used for cultivar identification (Saker et al, 2006;Chiu et al, 2010) and genetic analysis (Boronnikova et al, 2007;Silvestrini et al, 2008;Bhau et al, 2009;Baysal et al, 2010), and although thousands of related papers have been published, it does not mean they have been easily used in genotyping. In fact, the situation is more serious than anticipated.…”

Section: Discussionmentioning

confidence: 99%

Methodology A new strategy employed for identification of sweet orange cultivars with RAPD markers

Sun

Mu²,

Jiang³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency of RAPD markers for cultivar identification. Thirteen effective primers were first screened from 54 RAPD arbitrary 11-mer primers based on their amplification products and amplified polymorphic bands; they were then used for PCR amplification of all 60 cultivars. All cultivars were manually and completely separated by the polymorphic bands appearing in DNA fingerprints from 13 primers; a CID of the 60 sweet orange cultivars was then constructed. This CID separated all the cultivars from each other, based on the polymorphic bands; the corresponding primers were marked in the correct positions on the sweet orange CID. The CID strategy facilitates the identification of fruit cultivars with DNA markers. This CID of sweet orange cultivars will be very useful for the protection of cultivar rights and for early identification of seedlings in the nursery industry.

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“…However, isolates belonging to different VCGs can also belong to the same clonal lineage (Baayen et al 2000). Several formae speciales of F. oxysporum have been characterized by VCG analysis (Lori et al 2004;Abo et al 2005), as well as by random amplified polymorphio DNA (RAPD) analysis (Alves-Santos et al 2007;Bayraktar et al 2008;Baysal et al 2010) and several studies have combined both markers (Vakalounakis et al 2004;Nagarajan et al 2006). This technique is appropriate for developing countries with inadequate facilities for molecular work and can rapidly determine genetic groups of many fungal pathogens and their relation to pathogenicity (Cumagun et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Relationship between pathogenicity, race and vegetative compatibility grouping among Algerian populations of Fusarium oxysporum f. sp. pisi causing pea wilt

Merzoug¹,

Belabid²

2018

Journal of Plant Protection Research

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Fusarium oxysporum f. sp. pisi (FOP) is a significant and destructive pathogen of field pea in Algeria. In the present study, 50 isolates of F. oxysporum f. sp. pisi, the causal agent of pea (Pisum sativum) wilt, collected from different parts of western Algeria and representing four races of the pathogen, were analyzed for virulence. The wilt incidence ranged from 6.66 to 88.33% on a highly susceptible cultivar (Little Marvel). Twenty-one isolates belonging to four races of FOP and one nonpathogenic F. oxysporum (FO) isolate were analyzed for vegetative compatibility in order to reveal the genetic structure of the population and to check the reliability of the method for the identification of physiological races of FOP. Obtained results showed that the FOP isolates could be classified into four main vegetative compatibility groups (VCGs) that corresponded to races l, 2A, 2B and 5. The race 6 isolate fell into the race 1 VCG. To our knowledge, this is the first such study in Algeria of its kind.

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Molecular Characterization of Fusarium oxysporum f. melongenae by ISSR and RAPD Markers on Eggplant

Cited by 57 publications

References 24 publications

Occurrence of Fusarium verticillioides and Fusarium musae on banana fruits marketed in Hungary

Occurrence of Fusarium verticillioides and Fusarium musae on banana fruits marketed in Hungary

Methodology A new strategy employed for identification of sweet orange cultivars with RAPD markers

Relationship between pathogenicity, race and vegetative compatibility grouping among Algerian populations of Fusarium oxysporum f. sp. pisi causing pea wilt

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