Relationship between pathogenicity, race and vegetative compatibility grouping among Algerian populations of Fusarium oxysporum f. sp. pisi causing pea wilt

Abstract

Fusarium oxysporum f. sp. pisi (FOP) is a significant and destructive pathogen of field pea in Algeria. In the present study, 50 isolates of F. oxysporum f. sp. pisi, the causal agent of pea (Pisum sativum) wilt, collected from different parts of western Algeria and representing four races of the pathogen, were analyzed for virulence. The wilt incidence ranged from 6.66 to 88.33% on a highly susceptible cultivar (Little Marvel). Twenty-one isolates belonging to four races of FOP and one nonpathogenic F. oxysporum (FO) isolate were analyzed for vegetative compatibility in order to reveal the genetic structure of the population and to check the reliability of the method for the identification of physiological races of FOP. Obtained results showed that the FOP isolates could be classified into four main vegetative compatibility groups (VCGs) that corresponded to races l, 2A, 2B and 5. The race 6 isolate fell into the race 1 VCG. To our knowledge, this is the first such study in Algeria of its kind.

Genetic and virulence variation in Fusarium oxysporum f. sp. cepae causing root and basal rot of common onion in Iran

“…melongenae isolates, in which the classification into groups based on ISSR and RAPD fingerprints showed genetic specificity and diversity among the isolates, supporting our results on FOC. Merzoug and Belabid () analysed fifty F . oxysporum f. sp.…”

“…cepae is of different genetic lineages and is a polyphyletic group. But, Bayraktar () and Merzoug and Belabid () reported a very high polymorphism on genetic diversity and population structure of F. oxysporum f. sp. cepaee , using RAPD markers.…”

“…Therefore, understanding genetic variations within the pathogen populations is imperative and should be considered as one of the first steps for the delineation of disease management programmes (McDonald & Linde, ). Different methods have been used to assess genetic variation within populations of FOC such as vegetative compatibility grouping (VCGs) (Swift, Wickliffe, & Schwartz, ), random amplified polymorphism DNA (RAPD) analysis (Bayraktar, ; Merzoug & Belabid, ), amplified fragment length polymorphisms (AFLPs) (Galván et al., ), restriction fragment length polymorphism (RFLP) (Bayraktar, Turkkan, & Dolar, ; Jana, Sharma, Prasad, & Arora, ), intersimple sequence repeats (ISSR) analysis of internal transcribed spacer (ITS) (Bayraktar et al., ; Dissanayake, Kashima, Tanaka, & Ito, ) and intergenic spacer (IGS) regions of rDNA (Bayraktar et al., ; Dissanayake et al., ; Sasaki, Nakahara, Tanaka, Shigyo, & Ito, ). Thus, these studies reveal that F. oxysporum f. sp.…”

“…Random amplified polymorphism DNA (RAPD) analysis being used for several formae speciales of F. oxysporum characterization (Abo et al., ; Alves‐Santos, Martínez‐Bermejo, Rodríguez‐Molina, & Díez, ; Bayraktar, ; Baysal et al., ; Merzoug & Belabid, ). RAPD technique is an appropriate tool, where there are inadequate facilities for molecular work.…”

“…RAPD technique is an appropriate tool, where there are inadequate facilities for molecular work. Moreover, it can rapidly determine genetic variability and characterization of many fungal pathogens relationships (Altinok, Can, & Altinok, ; Merzoug & Belabid, ). Intersimple sequence repeats (ISSR) analysis has provided many polymorphic DNA markers related to the intra‐ and interspecific differences of a wide variety of fungi (Altinok et al., ; Bayraktar, ; Bayraktar & Dolar, ; Debbi et al., ).…”