Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France)

Biendo, M.; Adjidé, Crespin; Castelain, Sandrine; Belmekki, Mohamed; Rousseau, Florence; Slama, Michel; Ganry, O.; Schmit, J. L.; Eb, F.

doi:10.1155/2010/150464

Cited by 25 publications

(30 citation statements)

References 37 publications

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“…In France, the virulence determinants have evaluated regarding glycopeptide resistance. Biendo and colleagues demonstrated this gene in 29.8% of vancomycin resistance of E. faecium (13). In contrast, the present findings specified hyl gene in 21.1% (11/63 E. faecalis and 7/22 E. faecium isolates) of all enterococci isolates, and no MDR isolate was found related to this gene.…”

Section: Discussioncontrasting

confidence: 86%

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Shahraki

Mousavi

2017

Jundishapur J Microbiol

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Background: The enterococci are responsible for infections including bacteremia and endocarditis which are usually resistant to multiple antibiotics. This nosocomial agent probably harbors putative virulence factors which increases their capability to colonize hospitalized patients. Objectives: This study was aimed in order to find the frequency of various virulence factors in enterococci and their relationship with multidrug resistance (MDR). Methods: The samples were collected from different hospital wards including; Intensive care unit (ICU), cardiac care unit (CCU), pediatrics department, internal wards, and transplantation. The isolates were detected by biochemical tests and in order to determine the antibiotic susceptibility pattern, disk diffusion agar (kirby-bauer) was accomplished. Then, MICs (Minimum inhibitory concentrations) of vancomycin were determined by E-test strips. For molecular examinations and detection of drug resistance genes, the simple polymerase chain reaction was used. The multiplex PCR was used in order to detect virulence factors. Results: Total of 85 isolates were obtained from one university teaching hospital in southeast of Iran. Approximately 95% of isolated which were from urine specimens and 34% of isolates were collected from pediatrics units at hospital. Tetracycline resistance (48%) has been observed with a high frequency and related to the tetM gene. Furthermore, eighteen isolates were recognized as MDR strains that carried vanA, aac (6)-Ie-aph (2)-Ia, ermB, and tetM genes. Among virulence factor genes, asa1 (69%) and gelE (55%) are more frequently observed in both species. In general, we found Enterococcus faecalis strains more prevalent. Also, E. faecium was related to antibiotic resistance genes in nosocomial infection. Conclusions:The data was indicated a high prevalence of multiple antibiotics resistance genes with virulence determinants in enterococci and also considered resistant isolate in pediatrics unit. The current results can be recommended in order to change new strategies for antibiotic therapy, because this serious pathogen is important for treatment and eradication in hospitals. Furthermore, the biofilm formation was regulated and constructed by virulence determinants that could be a candidate for enterococcal treatment.

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Section: Discussioncontrasting

confidence: 86%

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Shahraki

Mousavi

2017

Jundishapur J Microbiol

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“…However, in the United Kingdom, a hyl gene prevalence of 71% (20/28) was observed in E. faecium isolates [14,42,43]. We believe that the differences observed in the detection rates of the hyl gene are due to the region in which the samples were isolated.…”

Section: Discussionmentioning

confidence: 98%

Molecular analysis and distribution of multidrug-resistant Enterococcus faeciumisolates belonging to clonal complex 17 in a tertiary care center in Mexico City

et al. 2013

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BackgroundEnterococcus faecium has recently emerged as a multidrug-resistant nosocomial pathogen involved in outbreaks worldwide. A high rate of resistance to different antibiotics has been associated with virulent clonal complex 17 isolates carrying the esp and hyl genes and the purK1 allele.ResultsTwelve clinical vancomycin-resistant Enterococcus faecium (VREF) isolates were obtained from pediatric patients at the Hospital Infantil de México Federico Gómez (HIMFG). Among these VREF isolates, 58.3% (7/12) were recovered from urine, while 41.7% (5/12) were recovered from the bloodstream. The VREF isolates showed a 100% rate of resistance to ampicillin, amoxicillin-clavulanate, ciprofloxacin, clindamycin, chloramphenicol, streptomycin, gentamicin, rifampicin, erythromycin and teicoplanin. In addition, 16.7% (2/12) of the isolates were resistant to linezolid, and 66.7% (8/12) were resistant to tetracycline and doxycycline. PCR analysis revealed the presence of the vanA gene in all 12 VREF isolates, esp in 83.3% (10/12) of the isolates and hyl in 50% (6/12) of the isolates. Phylogenetic analysis via molecular typing was performed using pulsed-field gel electrophoresis (PFGE) and demonstrated 44% similarity among the VREF isolates. MLST analysis identified four different sequence types (ST412, ST757, ST203 and ST612).ConclusionThis study provides the first report of multidrug-resistant VREF isolates belonging to clonal complex 17 from a tertiary care center in Mexico City. Multidrug resistance and genetic determinants of virulence confer advantages among VREF in the colonization of their host. Therefore, the prevention and control of the spread of nosocomial infections caused by VREF is crucial for identifying new emergent subclones that could be challenging to treat in subsequent years.

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“…Plasmids are very dynamic structures, and these events demonstrate the plasticity of the MGE and the rearrangements that may occur during conjugation processes. Presence of glycopeptide encoded in sex-pheromone plasmids have been detected in Asia in enterococci from production animals (Tanimoto et al, 2005;Lim et al, 2006;Getachew et al, 2009;Harada et al, 2010) but only described in a few studies in Europe (Sabia et al, 2007;Haenni et al, 2009;Biendo et al, 2010). Further, since a unique plasmid was detected in the transconjugants, this plasmid presumable also co-transferred the erm(B) gene.…”

Section: Discussionmentioning

confidence: 99%

“…It is interesting to notice that the antimicrobial avoparcin was banned in Europe in 1999. However, glycopeptide resistance appears to still be present at low levels in production animals (DANMAP, 2006;Sorum et al, 2006), and GR E. faecalis have been isolated in low prevalence in food animals in Europe (Sabia et al, 2007;Haenni et al, 2009;Biendo et al, 2010). Macrolides are extensively used for animal production and also for the treatment of infections in humans.…”

Section: Discussionmentioning

confidence: 99%

Presence of Glycopeptide-Encoding Plasmids in Enterococcal Isolates from Food and Humans in Denmark

Migura-García

Sánchez-Valenzuela

Jensen

2011

Foodborne Pathogens and Disease

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Enterococci and especially glycopeptides-resistant enterococci (GRE) are a growing concern due to their ability to cause infections in hospitals. Transmission of antimicrobial resistance between reservoirs such as animals, meat, and humans are in most cases linked to transmission of mobile genetic elements (MGE) such as plasmids and transposons. Presence of MGE was tested in all GRE isolated from food in Denmark in 2005-2007 including the first vanA mediated Enterococcus faecalis isolated from food. The ability of these plasmids to transfer and persist among enterococci was investigated using newly developed techniques for classification of plasmids. Replicons associated with sex pheromone-inducible plasmids were detected in all GR E. faecalis, whereas GR Enterococcus faecium contained plasmids known to be widely distributed among enterococci. vanA resistance is common in E. faecium isolates from meat and animals in Europe and is rarely found in E. faecalis. This article describes the first characterization of MGE from vanA mediated E. faecalis, thus linking this resistance genotype to pheromone responding plasmids.

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Molecular Characterization of Glycopeptide-Resistant Enterococci from Hospitals of the Picardy Region (France)

Cited by 25 publications

References 37 publications

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Molecular analysis and distribution of multidrug-resistant Enterococcus faeciumisolates belonging to clonal complex 17 in a tertiary care center in Mexico City

Presence of Glycopeptide-Encoding Plasmids in Enterococcal Isolates from Food and Humans in Denmark

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