Molecular characterization of <i>Potato mop-top virus</i> isolates from China and Canada and development of RT-PCR differentiation of two sequence variant groups

Hu, Xinxi; Dickison, Virginia; Liang, Yan; He, Changzheng; Singh, Mathuresh; Yang, Yanli; Xiong, Xingyao; Nie, Xianzhou

doi:10.1080/07060661.2016.1189968

Cited by 7 publications

(4 citation statements)

References 53 publications

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“…PMTV continues to pose a significant threat to potato production, as exemplified by the recent recognition of the presence of the virus in Canada (Hu et al ., ) and the USA (Ramesh et al ., ). Therefore, there is a growing need to understand the variability of the virus at a global scale.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, there is a growing need to understand the variability of the virus at a global scale. Previous studies examining the genetic composition of PMTV isolates in Europe, Asia and North America have reported low variability of the virus (Beuch et al ., ; Hu et al ., ; Ramesh et al ., ). However, it remains unclear whether low variability of PMTV is typical for all parts of the world.…”

Section: Discussionmentioning

confidence: 99%

“…Surprisingly, some RNA viruses with multipartite genomes, e.g. Potato mop‐top virus (PMTV), show variability as low as 0%–2% depending on the isolate (Beuch et al ., ; Hu et al ., ; Latvala‐Kilby et al ., ; Ramesh et al ., ). We speculated that the high genomic stability and low sequence variation of PMTV, to preserve genome integrity, might be a result of constraints imposed by the need to transport three RNA genomic components and the mode of PMTV movement in the plant as polar virions, which require rather intricate interactions between the minor coat protein (also known as CP‐RT), viral movement protein and, possibly, certain host factors at one extremity of the virus particles, leaving little room for variation (Torrance et al ., ).…”

Section: Introductionmentioning

confidence: 99%

“…PMTV infection is characterized by typical ‘spraing’ symptoms in the tuber, which consist of necrotic rings and flecks in the tuber flesh (Calvert and Harrison, ). The occurrence of the virus has been reported from many parts of the world, including Nordic countries, the Americas and parts of Asia (Hu et al ., ; Ramesh et al ., ; Santala et al ., ). The recent discovery of new genetic variants of the virus in USA and Colombia raises concern about the epidemiological risk this might bring to territories in which these variants are not present (Gil et al ., ; Ramesh et al ., ).…”

Section: Introductionmentioning

confidence: 99%

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Molecular and pathobiological characterization of 61 Potato mop‐top virus full‐length cDNAs reveals great variability of the virus in the centre of potato domestication, novel genotypes and evidence for recombination

Kalyandurg

Gil

Lukhovitskaya

et al. 2017

Molecular Plant Pathology

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SUMMARYThe evolutionary divergence of Potato mop-top virus (PMTV), a tri-partite, single-stranded RNA virus, is exceptionally low, based on the analysis of sequences obtained from isolates from Europe, Asia and North America. In general, RNA viruses exist as dynamic populations of closely related and recombinant genomes that are subjected to continuous genetic variation. The reason behind the low genetic variation of PMTV remains unclear. The question remains as to whether the low variability is a shared property of all PMTV isolates or is a result of the limited number of isolates characterized so far. We hypothesized that higher divergence of the virus might exist in the Andean regions of South America, the centre of potato domestication. Here, we report high variability of PMTV isolates collected from 12 fields in three locations in the Andean region of Peru. To evaluate PMTV genetic variation in Peru, we generated full-length cDNA clones, which allowed reliable comparative molecular and pathobiological characterization of individual isolates. We found significant divergence of the CP-RT and 8K sequences. The 8K cistron, which encodes a viral suppressor of RNA silencing, was found to be under diversifying selection. Phylogenetic analysis determined that, based on the CP-RT sequence, all PMTV isolates could be categorized into three separate lineages (clades). Moreover, we found evidence for recombination between two clades. Using infectious cDNA clones of the representatives of these two clades, as well as reassortants for the RNA-CP genomic component, we determined the pathobiological differences between the lineages, which we coined as S (for severe) and M (for mild) types. Interestingly, all isolates characterized previously (from Europe, Asia and North America) fall into the S-type clade, whereas most of the Peruvian isolates belong to the M-type. Taken together, our results support the notion of the single introduction of PMTV from the centre of potato origin to Europe, and subsequent spread of the S-type into Asia and USA. This is also supported by the suggested novel classification of isolates based on genetic constellations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%