Molecular Characterization of Imipenem‐Resistant Pseudomonas aeruginosa in Hiroshima, Japan

Ohara, Masaru; Kouda, Shuntaro; Onodera, Makoto; Fujiue, Yoshihiro; Sasaki, Megumi; Kohara, Tadahiro; Kashiyama, Seiya; Hayashida, Shizue; Kadono, Manami; Komatsuzawa, Hitoshi; Gotoh, Naomasa; Usui, Tomofusa; Itaha, Hideyuki; Kuwabara, Masao; Yokoyama, Takashi; Sugai, Motoyuki

doi:10.1111/j.1348-0421.2007.tb03908.x

Cited by 21 publications

(17 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Increasing resistance to the various antipseudomonas agents has been reported worldwide and this poses a serious problem in therapeutic management of P. aeuroginosa infections (Carmeli et al, 1999, Obritsch et al, 2004. Approximately 58% of P. aeruginosa isolates in our study were multi-drug resistant strains, however the prevalence of multi-drug resistance isolates of P. aeruroginosa in the other studies are much lower than our study (Ohara et al, 2007).…”

Section: Discussioncontrasting

confidence: 54%

Phenotypic detection of metallo--lactamase producing Pseudomonas aeruginosa isolated from Urmia hospitals

Jazani¹,

Zahedi²,

Garebagi³

2012

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

Detection of metallo-β-lactamase producing Pseudomonas aeruginosa is crucial for the optimal treatment of patients; however there are limited studies on metallo-β-lactamase producing P. aeruginosa isolates from West Azarbayejan, Iran. This study was designed to detect the metallo-β-lactamase in P. aeruginosa isolates. One hundred isolates were collected from clinical specimens submitted to hospital diagnostic laboratories in Urmia/Iran from July to September 2010. The susceptibilities of the isolates to different classes of antibiotics were tested using Müller-Hinton agar disk diffusion method. All isolates of P. aeruginosa were subjected to determination of minimum inhibitory concentrations )MICs) against Imipenem. Imipenem non-susceptible isolates were investigated for metallo-β-lactamase production by the combined disk method. The rates of resistances to antibiotics, as were determined, is as follows: kanamycin (91%), Tobramycin (34%), Ciprofloxacin (16%), Colistin (68%), Ticarcillin (46%), Amikacin (16%), Norfloxacin (23%), gentamicin (33%), Ceftazidime (62%), Ceftizoxime (69%), and Cefepime (39%). Seventy nine isolates (79%) were sensitive (MIC ≤ 4 mg/L) and 21 isolate (21%) were resistant to Imipenem (MIC ≥ 8 mg/L). The rates of resistance to different antibiotics were much higher in Imipenem resistant isolates. Detection of metallo-β-lactamase producing isolates among Imipenem non-susceptible isolates of P. aeruginosa revealed that seven isolates (33.3%) were metallo-β-lactamase positive. Metallo-β-lactamase positive isolates showed high resistances to all tested antibiotics. This result suggests that metallo-β-lactamase producing isolates in hospitals may cause serious infections which can lead to failure in patient's antibiotic therapy.

show abstract

Section: Discussioncontrasting

confidence: 54%

Phenotypic detection of metallo--lactamase producing Pseudomonas aeruginosa isolated from Urmia hospitals

Jazani¹,

Zahedi²,

Garebagi³

2012

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…A number of reports of opportunistic infections in cancer patients have been published (1,2,5,7). However, the oral conditions of such patients after oral cancer therapy (so-called "follow up-patients") are not well known.…”

Section: Introductionmentioning

confidence: 99%

“…P aeruginosa is another representative bacterium that causes opportunistic infections (5). It is found in soil, water, and skin flora and can survive in poor environments including hypoxic atmosphere.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of drug-resistant opportunistic microorganisms in oral cavity after treatment for oral cancer

et al. 2013

Self Cite

View full text Add to dashboard Cite

Drug-resistant opportunistic infectionsmay cause health problems in immunocompromised hosts. Representative microorganisms in opportunistic infections of the oral cavity are Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. We investigated the prevalence of drugresistant opportunistic microorganisms in elderly adults receiving follow-up examinations after primary treatment of oral cancer. Oral microorganisms were collected from patients satisfactorily treated for oral cancer (defined as good outcomes to date) and a group of healthy adults (controls). After identification of microorganisms, the prevalence of drug-resistant microorganisms was studied. Pulsedfield gel electrophoresis (PFGE) and staphylococcal cassette chromosome mec (SCCmec) typing were also performed for methicillin-resistant S aureus (MRSA). Statistical analysis revealed no significant differences in the prevalences of the three microorganisms between the groups. Surprisingly, 69.2% of S aureus isolates showed oxacillin resistance, suggesting that MRSA colonization is increasing among older Japanese. These MRSA isolates possessed SCCmec types II and IV but no representative toxin genes. Our results indicate that a basic infection control strategy, including standard precautions against MRSA, is important for elderly adults, particularly after treatment for oral cancer. (J Oral Sci 55, [145][146][147][148][149][150][151][152][153][154][155] 2013)

show abstract

“…due to metallo-β-lactamases (MBLs) production have been reported from different regions. [1][2][3] The emergence of these MBLs in gram negative bacilli is becoming a therapeutic challenge as these enzymes possess high hydrolytic activity that leads to degradation of higher generation cephalosporins. Moreover, the treatment alternatives are unavailable, or expensive/toxic with poor outcome.…”

mentioning

confidence: 99%

Metallo-β-lactamase-producing clinical isolates of Acinetobacter species and Pseudomonas aeruginosa from intensive care unit patients of a tertiary care hospital

Irfan¹,

Zafar²,

Guhar³

et al. 2008

Indian J Med Microbiol

View full text Add to dashboard Cite

Prompt detection of metallo-β-lactamase (MBL) producing isolates is necessary to prevent their dissemination. Frequency of MBLs producing strains among multidrug resistant (MDR) Acinetobacter species and Pseudomonas aeruginosa was evaluated in critical care patients using imipenem-EDTA disk method. One hundred MDR Acinetobacter spp. and 42 Pseudomonas aeruginosa were checked for MBL production, from January to June 2001. MBL was produced by 96.6 % of imipenem-resistant Acinetobacter isolates, whereas 100% imipenem-resistant Pseudomonas aeroginosa isolates were MBL producers. Carbapenem resistance in MDR Acinetobacter spp. and Pseudomonas aeruginosa isolates in this study was due to MBLs. This calls for strict infection control measures to prevent further dissemination. Key words: Acinetobacter spp, metallo-β-lactamase, Pseudomonas aeruginosaCarbapenem group of antibiotics play a vital role in the management of hospital-acquired gram negative infections, because of their broad spectrum activity and stability to hydrolysis by most of the β-lactamases, including extended spectrum β-lactamases (ESBLs). Nosocomial outbreaks of carbapenem-resistant Pseudomonas aeruginosa and Acinetobacter spp. due to metallo-β-lactamases (MBLs) production have been reported from different regions. [1][2][3] The emergence of these MBLs in gram negative bacilli is becoming a therapeutic challenge as these enzymes possess high hydrolytic activity that leads to degradation of higher generation cephalosporins. Moreover, the treatment alternatives are unavailable, or expensive/toxic with poor outcome.[4] Plasmid mediated MBL genes spread rapidly to other species of gram negative bacilli; [5] therefore rapid detection of metallo-ß-lactamases production is necessary to modify therapy and to initiate effective infection control to prevent their dissemination. To date there has been one report from Pakistan suggesting presence of MBL producing isolate.[6] There is however need for a systematic study to assess the extent of this form of resistance amongst our isolates. The purpose of this study was to evaluate the metallo-ß-lactamase (MBL) production among multidrug resistant (MDR) Acinetobacter spp. and Pseudomonas aeruginosa, isolated from clinical specimens of intensive care unit patients. Materials and MethodsThis study was conducted in the clinical microbiology laboratory of The Aga Khan University Hospital, Karachi, Pakistan. One hundred MDR clinical isolates of Acinetobacter species and 42 Pseudomonas aeruginosa were studied for MBL production. These organisms were isolated from respiratory secretions, wound swabs, urine and blood culture specimens of intensive care unit patients, admitted from January to April 2001. A multidrug resistant isolate was deÞ ned as resistance to two or more drugs or drug classes of therapeutic relevance. [7] All the isolates of Pseudomonas aeruginosa were characterised to species level using standard procedures. Similarly, Acinetobacter isolates were characterised to the genus level using these proced...

show abstract

Molecular Characterization of Imipenem‐Resistant Pseudomonas aeruginosa in Hiroshima, Japan

Cited by 21 publications

References 26 publications

Phenotypic detection of metallo--lactamase producing Pseudomonas aeruginosa isolated from Urmia hospitals

Phenotypic detection of metallo--lactamase producing Pseudomonas aeruginosa isolated from Urmia hospitals

Prevalence of drug-resistant opportunistic microorganisms in oral cavity after treatment for oral cancer

Metallo-β-lactamase-producing clinical isolates of <i> Acinetobacter</i> species and <i> Pseudomonas aeruginosa</i> from intensive care unit patients of a tertiary care hospital

Contact Info

Product

Resources

About