Molecular characterization of isolates of Beauveria bassiana obtained from overwintering and summer populations of Sunn Pest (Eurygaster integriceps)

Kouvelis, Vassili N.; Ghikas, Dimitri V.; Edgington, Steve; Typas, Milton A.; Moore, David

doi:10.1111/j.1472-765x.2008.02331.x

Cited by 15 publications

(12 citation statements)

References 24 publications

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“…An increasing number of studies point towards a broad correlation of fungal isolates with their place of origin and/or habitats [e.g., [18,21,30,59,60]]. Obviously, the factors that can influence B. bassiana population structures are many and can include: climate conditions, the range of temperatures in which the various isolates can grow in nature, humidity levels, UV exposure, habitat, cropping system and soil properties [18,27,59,61].…”

Section: Discussionmentioning

confidence: 99%

“…In other mitosporic entomopathogenic fungi, such as Metarhizium [27], Lecanicillium [28] and Nomurea [29], mtDNA data compared favourably to data based on ITS combined with a single nuclear gene, for applications in phylogeny, taxonomy and species or strain -identification. In Beauveria , the use of mtDNA RFLPs or partial mtDNA sequences suggested that mtDNA can be equally useful for such studies [2,30]. …”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic and biogeographic implications inferred by mitochondrial intergenic region analyses and ITS1-5.8S-ITS2 of the entomopathogenic fungi Beauveria bassiana and B. brongniartii

2010

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BackgroundThe entomopathogenic fungi of the genus Beauveria are cosmopolitan with a variety of different insect hosts. The two most important species, B. bassiana and B. brongniartii, have already been used as biological control agents of pests in agriculture and as models for the study of insect host - pathogen interactions. Mitochondrial (mt) genomes, due to their properties to evolve faster than the nuclear DNA, to contain introns and mobile elements and to exhibit extended polymorphisms, are ideal tools to examine genetic diversity within fungal populations and genetically identify a species or a particular isolate. Moreover, mt intergenic region can provide valuable phylogenetic information to study the biogeography of the fungus.ResultsThe complete mt genomes of B. bassiana (32,263 bp) and B. brongniartii (33,920 bp) were fully analysed. Apart from a typical gene content and organization, the Beauveria mt genomes contained several introns and had longer intergenic regions when compared with their close relatives. The phylogenetic diversity of a population of 84 Beauveria strains -mainly B. bassiana (n = 76) - isolated from temperate, sub-tropical and tropical habitats was examined by analyzing the nucleotide sequences of two mt intergenic regions (atp6-rns and nad3-atp9) and the nuclear ITS1-5.8S-ITS2 domain. Mt sequences allowed better differentiation of strains than the ITS region. Based on mt and the concatenated dataset of all genes, the B. bassiana strains were placed into two main clades: (a) the B. bassiana s. l. and (b) the "pseudobassiana". The combination of molecular phylogeny with criteria of geographic and climatic origin showed for the first time in entomopathogenic fungi, that the B. bassiana s. l. can be subdivided into seven clusters with common climate characteristics.ConclusionsThis study indicates that mt genomes and in particular intergenic regions provide molecular phylogeny tools that combined with criteria of geographic and climatic origin can subdivide the B. bassiana s.l. entomopathogenic fungi into seven clusters with common climate characteristics.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Phylogenetic and biogeographic implications inferred by mitochondrial intergenic region analyses and ITS1-5.8S-ITS2 of the entomopathogenic fungi Beauveria bassiana and B. brongniartii

2010

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“…In other fungal genera, like Lecanicillium , Verticillium, and Beauveria , the use of mitochondrial (mt) genes and mt intergenic regions proved an extremely useful tool to reveal species differences within a genus and even helped to resolve taxonomic ambiguities (e.g., the small rRNA subunit ( rns ), the NADH dehydrogenase subunits 1 ( nad 1) and 3 ( nad 3), the mt intergenic domains nad 3– atp 9 and the atp 6- rns ) [25–27]. Similarly, other nuclear regions/genes like the RNA polymerase II largest subunit gene ( rpb 1) and the IGS region of the rRNA repeat also proved to be excellent tools for discriminating species within Metarhizium, Verticillium and Aspergillus [23, 27, 28].…”

Section: Introductionmentioning

confidence: 99%

A Phylogenetic Analysis of Greek Isolates ofAspergillusSpecies Based on Morphology and Nuclear and Mitochondrial Gene Sequences

Krimitzas

Pyrri

Kouvelis

et al. 2013

BioMed Research International

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Aspergillus species originating from Greece were examined by morphological and molecular criteria to explore the diversity of this genus. The phylogenetic relationships of these species were determined using sequences from the ITS and IGS region of the nuclear rRNA gene complex, two nuclear genes (β-tubulin (benA) and RNA polymerase II second largest subunit (rpb2)) and two mitochondrial genes (small rRNA subunit (rns) and cytochrome oxidase subunit I (cox1)) and, where available, related sequences from databases. The morphological characters of the anamorphs and teleomorphs, and the single gene phylogenetic trees, differentiated and placed the species examined in the well-supported sections of Aenei, Aspergillus, Bispori, Candidi, Circumdati, Clavati, Cremei, Flavi, Flavipedes, Fumigati, Nidulantes, Nigri, Restricti, Terrei, Usti, and Zonati, with few uncertainties. The combined use of the three commonly employed nuclear genes (benA, rpb2, and ITS), the IGS region, and two less often used mitochondrial gene sequences (rns and cox1) as a single unit resolved several taxonomic ambiguities. A phylogenetic tree was inferred using Neighbour-Joining, Maximum Parsimony, and Bayesian methods. The strains examined formed seven well-supported clades within the genus Aspergillus. Altogether, the concatenated nuclear and mitochondrial sequences offer additional tools for an improved understanding of phylogenetic relationships within this genus.

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“…( sensu lato ), and also that EF1-α sequences provide adequate information for the inference of relationships in this genus [8]. Studies on the genetic variability of BCAs such as B. bassiana are crucial for the development of molecular tools for their monitoring in the natural environment [6]. …”

Section: Introductionmentioning

confidence: 99%

Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution

et al. 2011

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BackgroundThe entomopathogenic anamorphic fungus Beauveria bassiana is currently used as a biocontrol agent (BCA) of insects. Fifty-seven Beauveria bassiana isolates -53 from Spain- were characterized, integrating group I intron insertion patterns at the 3'-end of the nuclear large subunit ribosomal gene (LSU rDNA) and elongation factor 1-alpha (EF1-α) phylogenetic information, in order to assess the genetic structure and diversity of this Spanish collection of B. bassiana.ResultsGroup I intron genotype analysis was based on the four highly conserved insertion sites of the LSU (Ec2653, Ec2449, Ec2066, Ec1921). Of the 16 possible combinations/genotypes, only four were detected, two of which were predominant, containing 44 and 9 members out of 57 isolates, respectively. Interestingly, the members of the latter two genotypes showed unique differences in their growth temperatures. In follow, EF1-α phylogeny served to classify most of the strains in the B. bassiana s.s. (sensu stricto) group and separate them into 5 molecular subgroups, all of which contained a group I intron belonging to the IC1 subtype at the Ec1921 position. A number of parameters such as thermal growth or origin (host, geographic location and climatic conditions) were also examined but in general no association could be found.ConclusionMost Spanish B. bassiana isolates (77.2%) are grouped into a major phylogenetic subgroup with word-wide distribution. However, high phylogenetic diversity was also detected among Spanish isolates from close geographic zones with low climatic variation. In general, no correlation was observed between the molecular distribution and geographic origin or climatic characteristics where the Spanish B. bassiana isolates were sampled.

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Molecular characterization of isolates of Beauveria bassiana obtained from overwintering and summer populations of Sunn Pest (Eurygaster integriceps)

Cited by 15 publications

References 24 publications

Phylogenetic and biogeographic implications inferred by mitochondrial intergenic region analyses and ITS1-5.8S-ITS2 of the entomopathogenic fungi Beauveria bassiana and B. brongniartii

Phylogenetic and biogeographic implications inferred by mitochondrial intergenic region analyses and ITS1-5.8S-ITS2 of the entomopathogenic fungi Beauveria bassiana and B. brongniartii

A Phylogenetic Analysis of Greek Isolates ofAspergillusSpecies Based on Morphology and Nuclear and Mitochondrial Gene Sequences

Genetic analyses place most Spanish isolates of Beauveria bassiana in a molecular group with word-wide distribution

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