Molecular characterization of measles viruses in Turkey (2010–2011): First report of genotype D9 involved in an outbreak in 2011

Kalaycioglu, Atila T.; Baykal, Atakan; Güldemir, Dilek; Bakkaloğlu, Zekiye; Korukluoğlu, Gülay; Coskun, Aslihan; Torunoğlu, Mehmet Ali; Ertek, Mustafa; Durmaz, Rıza

doi:10.1002/jmv.23714

Cited by 8 publications

(9 citation statements)

References 19 publications

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“…RNA was purified from urine or throat swabs samples using a commercial viral RNAeasy Kit (Qiagen, Hilden, Germany) in accordance with manufacturer's recommendations. Like previously reported, RT‐PCR (QIAGEN) amplified 590 base pairs (bp) of the C‐terminal region of the N gene of viruses using the primer sequences n1 and n2 as described in the previous studies [Kühne et al, ; Kalaycioglu et al, ; Metin et al, ].…”

Section: Methodsmentioning

confidence: 99%

“…DNA was purified using Agencourt AMPure XP‐PCR Purification (Beckman Coulter Company, MA) as recommended by manufacturer's instructions. Sequencing reactions was set up by using cycle sequencing Quick Start Kit (Beckman Coulter) and conditions were followed as previously described [Kalaycioglu et al, , ]. Twenty microliters of DNA from each sample was subjected for sequencing with a CEQ 8000 Genetic Analyser (Beckman Coulter).…”

Section: Methodsmentioning

confidence: 99%

“…During 2011, MV outbreaks were reported in 36 of the 53 WHO–EUR countries [WHO, ]. One hundred and eleven cases were reported in Turkey, primarily in the Istanbul province, and genotype D9 viruses were identified [Kalaycioglu et al, ]. Measles outbreaks continued to occur in subsequent years in Europe, with 27,134 measles cases in 41 WHO–EUR countries in 2012, and 31,250 cases in 38 WHO–EUR countries in 2013, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Towards measles elimination: Phylogenetic analysis of measles viruses in Turkey (2012-2013) and identification of genotype D8

Kalaycioglu

Yolbakan²,

Güldemir³

et al. 2016

J. Med. Virol.

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Molecular characterization of different measles virus (MV) strains is essential to combat the disease. Sixty measles MV strains were obtained from throat swabs or urine of patients in Turkey between 2012 and 2013 and characterized. MV RNA sequences (n = 60) were analysed for 456 nucleotides representing hypervariable domain of the nucleoprotein (N) gene. Of the 60 strains analysed 53 were the D8 genotype, 6 were B3, 1 was D4, and 1 was A. This report describes MV genotype D8 that was involved in a measles outbreak in Turkey. Sequences of most genotype D8 strains (n = 51) were identical to the sequence of variant D8-Frankfurt-Main, which has been associated with outbreaks throughout Europe. Despite the lack of epidemiologic information, a phylogenetic analysis suggested that the genotype D8 MV may have been brought to Turkey from elsewhere. Phylogenetic and epidemiological findings suggested that strains identified in tourists and associated with importation included one strain of genotype D8, one strain of genotype B3, and one strain of genotype D4. These findings from the 2012 to 2013 outbreak in Turkey confirm that pockets of unimmunised individuals are making the country susceptible to measles outbreaks. To prevent further outbreaks, deliberate and sustained effort must be made to reach, and immunise susceptible age groups. Towards measles elimination process, continued molecular surveillance of measles strains in Turkey will help identify transmission patterns of virus and evaluate vaccination efforts. J. Med. Virol. 88:1867-1873, 2016. © 2016 Wiley Periodicals, Inc.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Towards measles elimination: Phylogenetic analysis of measles viruses in Turkey (2012-2013) and identification of genotype D8

Kalaycioglu

Yolbakan²,

Güldemir³

et al. 2016

J. Med. Virol.

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“…4 Ülkemizde 2011 yılında büyük çoğunluğu İs-tanbul'da görülen ve yurt dışından gelen (importe) olgulardan kaynaklandığı düşünülen bir salgın bildirilmiştir. 5 Kızamık olgularının kümelendiği iller ise Amasya, Ağrı, Diyarbakır, Elazığ, Kars, Kayseri, Konya, Kütahya, Samsun, Siirt, Van, Yozgat, Kilis, Muş, Iğdır, İzmir, Adıyaman, Hakkari, Gümüşhane ve Osmaniye idi. Türkiye'deki 2012-2013 salgı-nında kızamık virüsünün genotipi D8 ve B3 idi.…”

Section: Olguunclassified

An Evaluation of the Measles Among Adult Age Groups with Four Measles Cases Followed in Our Clinic: Case Report

Kiliç¹,

Kaya²,

Özdemir³

et al. 2015

Turkiye Klinikleri J Case Rep

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“…This technique can elucidate the spatial and temporal transmission dynamics of microbes, as has been done in studies of avian influenza virus [8], human immunodeficiency virus [9], hepatitis C virus [10], and Salmonella typhimurium [11]. Although there have been many epidemiological reports about inter-country transmission of measles [12,13,14,15], systematic analysis of the global transmission dynamics of MV has been limited.…”

Section: Introductionmentioning

confidence: 99%

Global Transmission Dynamics of Measles in the Measles Elimination Era

Furuse

Oshitani

2017

Viruses

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Although there have been many epidemiological reports of the inter-country transmission of measles, systematic analysis of the global transmission dynamics of the measles virus (MV) is limited. In this study, we applied phylogeographic analysis to characterize the global transmission dynamics of the MV using large-scale genetic sequence data (obtained for 7456 sequences) from 115 countries between 1954 and 2015. These analyses reveal the spatial and temporal characteristics of global transmission of the virus, especially in Australia, China, India, Japan, the UK, and the USA in the period since 1990. The transmission is frequently observed, not only within the same region but also among distant and frequently visited areas. Frequencies of export from measles-endemic countries, such as China, India, and Japan are high but decreasing, while the frequencies from countries where measles is no longer endemic, such as Australia, the UK, and the USA, are low but slightly increasing. The world is heading toward measles eradication, but the disease is still transmitted regionally and globally. Our analysis reveals that countries wherein measles is endemic and those having eliminated the disease (apart from occasional outbreaks) both remain a source of global transmission in this measles elimination era. It is therefore crucial to maintain vigilance in efforts to monitor and eradicate measles globally.

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Molecular characterization of measles viruses in Turkey (2010–2011): First report of genotype D9 involved in an outbreak in 2011

Cited by 8 publications

References 19 publications

Towards measles elimination: Phylogenetic analysis of measles viruses in Turkey (2012-2013) and identification of genotype D8

Towards measles elimination: Phylogenetic analysis of measles viruses in Turkey (2012-2013) and identification of genotype D8

An Evaluation of the Measles Among Adult Age Groups with Four Measles Cases Followed in Our Clinic: Case Report

Global Transmission Dynamics of Measles in the Measles Elimination Era

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