Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay

Félix, María L.; Muñoz-Léal, Sebastián; Carvalho, Luis; Queirolo, Diego; Remesar, Susana; Nava, Stefano; Armúa-Fernández, María Teresa; Venzal, José M.

doi:10.1016/j.crpvbd.2021.100022

Cited by 8 publications

(4 citation statements)

References 41 publications

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“…This divergence may be due to the biogeographic isolation of these sequences, since they come from the physiographic region Northern mountain ranges and plains, where components of the geographical space might cause isolation and deter connection with other physiographic regions in Mexico [ 37 ]. This phenomenon is similar to that found in Uruguay, where E. canis isolates were molecularly characterized and different genotypes correlated to areas of geographical isolation were observed [ 38 ]. However, not only the 16S gene was characterized, but also the dsb and groEL genes were included for characterization, increasing the probability of finding differences in the genetic sequences of the gene fragments used.…”

Section: Discussionsupporting

confidence: 86%

Molecular Detection and Characterization of Ehrlichia canis Isolates from Three Geographic Regions in Mexico: A Retrospective Study

Lira-Amaya,

Beristain-Ruiz,

Racanco-Delgado

et al. 2023

Life

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Canine monocytic ehrlichiosis (CME) is the most common tick-borne disease affecting domestic dogs and other wild canids. It has a worldwide distribution and is associated with the presence of the brown dog tick. Few studies have been conducted in Mexico to identify and characterize Ehrlichia canis genetic variability. In the present study, 111 dogs of different sex, breed, and age from three geographic regions in Mexico were included. All of them had a previous history of tick infestation and/or the presence of one or more clinical signs compatible with CME. All dogs were tested by a commercial ELISA and nested PCR assay for the detection of E. canis. In addition, we analyzed the E. canis genetic diversity from the 16S rRNA gene sequences obtained in this study, along with 15 additional sequences described for E. canis in Mexico and obtained from GeneBank. Serological detection by commercial ELISA results showed overall infection rates of 85.58% (95/111), including 73.1% (30/41) in samples from Guerrero state; 75% (15/20) in Morelos; and 100% (50/50) in Chihuahua. On the other hand, molecular detection (nPCR assay) showed 31.5% (35/111) overall infection rate, with 41.4% (17/41) in Guerrero state; 55% (11/20) in Morelos; and 14% (7/50) in Chihuahua. We observed a high 16S rRNA gene sequence conservancy in most of the E. canis isolates in the three geographical areas from Mexico, including those analyzed in this research, suggesting a common geographic origin among isolates.

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Section: Discussionsupporting

confidence: 86%

Molecular Detection and Characterization of Ehrlichia canis Isolates from Three Geographic Regions in Mexico: A Retrospective Study

Lira-Amaya,

Beristain-Ruiz,

Racanco-Delgado

et al. 2023

Life

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“…Furthermore, although A. longirostre, Dermacentor spp., Haemaphysalis spp., Ixodes spp., and Rhipicephalus spp. are considered non-anthropophilic, they can bite humans and are potential vectors of zoonoses, requiring further research (Labruna et al, 2005;Guglielmone et al, 2006;Labruna et al, 2007;Hun et al, 2008;Serra-Freire et al, 2011;Reck et al, 2018;Carvalho et al, 2020;Flores et al, 2020;Muñoz-Leal et al, 2020;Félix et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, recent studies have identified potential zoonotic agents in Ixodes ticks in Brazil and neighboring countries (Carvalho et al, 2020;Flores et al, 2020;Muñoz-Leal et al, 2020;Félix et al, 2021). A spirochete, Borrelia burgdorferi sensu lato (Bbsl), was recently detected in Ixodes paranaensis ticks collected in Ibitipoca State Park, MG.…”

Section: Discussionmentioning

confidence: 99%

Diversity of tick species (Acari: Ixodidae) in military training areas in Southeastern Brazil

Soares

Araújo

Cordeiro

et al. 2022

Rev. Bras. Parasitol. Vet.

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Tick-borne pathogens belong to one of the two main groups of occupational biohazards, and occupational exposure to such agents puts soldiers at risk of zoonotic infections, such as those caused by rickettsiae. There are few studies on acarological fauna and occupational risk in military areas in Brazil. Thus, the present study aimed to analyze the diversity of ticks present in the military training areas of municipalities in the Southeast Region of Brazil. The ticks were collected from the selected areas using the dragging and flagging techniques as well as by visual detection on the operators’ clothing, and environmental information was also recorded. A total of ten species were collected from the 66 surveyed areas, belonging to five genera and nine species: Amblyomma sculptum, Amblyomma dubitatum, Amblyomma brasiliense, Amblyomma longirostre, Amblyomma aureolatum, Dermacentor nitens, Rhipicephalus spp., Ixodes spp. and Haemaphysalis spp. The frequent presence of tick species in military training areas along with traces and sightings of wild animals, most commonly capybaras (Hydrochoerus hydrochaeris), in most of the studied areas, indicates high levels of exposure of the military to tick vectors of spotted fever group rickettsiae and the possible occurrence of infections among the troops.

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“…Los muestreos se realizaron entre los meses de junio y octubre de 2016 con una salida de campo adicional en septiembre de 2017 en la región norte de Uruguay, comprendiendo a los departamentos de Salto, Rivera y Tacuarembó. Las garrapatas fueron colectadas sobre la vegetación mediante la técnica de "bandereo o arrastre" pasando un trozo de tela blanca de un metro de ancho por 1,2 de largo, la cual se revisaba cada 5 a 10 metros (Félix et al, 2021). Además, se obtuvieron garrapatas sobre perros en establecimientos rurales, en zorros hallados recientemente atropellados en rutas y sobre un humano durante uno de los muestreos (Tabla 1).…”

Section: Materiales Y Métodosunclassified

Parasitismo de Amblyomma aureolatum (Pallas, 1772) (Acari: Ixodidae) en humanos y determinación molecular de organismos rickettsiales y piroplasmas en el norte de Uruguay

Alvez,

Felix,

Queirolo

et al. 2023

FAVET

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Amblyomma aureolatum (Pallas, 1772) se distribuye en Sudamérica y posee importancia médica y veterinaria. En Uruguay se encuentra ampliamente distribuida parasitando vertebrados silvestres y domésticos y se la relaciona a casos de rangeliosis canina. El objetivo de este trabajo fue determinar la presencia de organismos rickettsiales y piroplasmas en A. aureolatum del norte de Uruguay. Para ello se recolectaron 117 ninfas y adultos de A. aureolatum sobre zorros, perros y vegetación, así como una sobre un humano. Se extrajo ADN de las garrapatas obtenidas de animales y vegetación para realizar diferentes PCR utilizando cebadores dirigidos a genes para la detección de ADN de organismos Rickettsiales (Anaplasmataceae, Rickettsiaceae y Midichloriaceae) así como protozoarios del Orden Piroplasmida. Como resultados se obtuvieron tres secuencias, una para el gen gltA con una identidad de entre 99,4 a 100% con Rickettsia bellii, otra para el gen 16S ARNr con un 100% de identidad con Candidatus Midichloria sp. y la restante con 100% de identidad con Rangelia vitalii para el gen 18S ARNr. Estos resultados corresponden a los primeros reportes de R. bellii para el país y de Ca. Midichloria sp. y R. vitalii en A. aureolatum. Además, se reporta el primer caso de A. aureolatum parasitando humanos en Uruguay.

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Molecular characterization of novel Ehrlichia genotypes in Ixodes auritulus from Uruguay

Cited by 8 publications

References 41 publications

Molecular Detection and Characterization of Ehrlichia canis Isolates from Three Geographic Regions in Mexico: A Retrospective Study

Molecular Detection and Characterization of Ehrlichia canis Isolates from Three Geographic Regions in Mexico: A Retrospective Study

Diversity of tick species (Acari: Ixodidae) in military training areas in Southeastern Brazil

Parasitismo de Amblyomma aureolatum (Pallas, 1772) (Acari: Ixodidae) en humanos y determinación molecular de organismos rickettsiales y piroplasmas en el norte de Uruguay

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