Molecular characterization of pestiviruses isolated from bovines in Chile

Pizarro-Lucero, José; Celedón, M.O.; Aguilera, Marcela; Calisto, Alejandra de

doi:10.1016/j.vetmic.2006.02.009

Cited by 41 publications

(32 citation statements)

References 33 publications

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“…Whether these results are representative for the entire country or not needs to be confirmed through further studies. The results further indicate, in contrast to previous studies (Pizarro-Lucero et al 2006), that BVDV 1 is the predominant genotype circulating in the bovine population in the Southern regions IX and X of Chile. The discrepancy probably being due in part to the limited number of strains included in our study, but possibly also to regional variations within the country.…”

Section: Resultscontrasting

confidence: 74%

“…Therefore, the efficacy of these vaccines to protect foetuses against infection under field conditions have been questioned (van Oirschot et al 1999, O'Rourke 2002, and field observations, where PI calves have been born in vaccinated herds, support this concern (Van Campen et al 2000, Gaede et al 2004, Graham et al 2004. A recent study of BVDV diversity from Chile demonstrated an unusually high percentage of genotype 2 (51.5%) among 33 BVDV field isolates collected in the central part of the country (Pizarro-Lucero et al 2006). Based on this finding the authors concluded that BVDV-2 possibly had circulated for a long time in the country.…”

Section: Resultsmentioning

confidence: 98%

“…Genetic diversity of BVDV isolates is therefore not only of academic interest, but has practical implications for surveillance and vaccine design. To date there are reports on genetic diversity of BVDV from many countries on all continents, and from South America a few studies have been reported from Argentina, Brazil and recently also from Chile (Jones et al 2001, Flores et al 2002, Pizarro-Lucero et al 2006. In this report we describe for the first time the genetic diversity of BVDV strains collected in Peru.…”

Section: Introductionmentioning

confidence: 85%

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Genetic diversity of bovine viral diarrhoea virus (BVDV) from Peru and Chile

et al. 2009

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Twenty-five BVDV strains, detected in serum from persistently infected cattle from Peru (n=15) and Chile (n=10) were genetically characterized. The phylogenetic analysis based on the 5' UTR showed that all 25 strains belonged to genotype 1. Twenty-three of the strains could further be subdivided into subtype 1b, and two out of ten Chilean strains into subtype 1a. In conclusion, in total 23 out of 25 strains analyzed were of genotype 1, subtype 1b. This is the predominant BVDV subtype in many countries all over the world, including USA. The close homology with previously described strains reflects the influence of livestock trade on the diversity of BVDV circulating within and between countries and continents. Peru and Chile have imported large numbers of cattle from USA and Europe, mostly with insufficient or lacking health documentation.

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Section: Resultscontrasting

confidence: 74%

Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 85%

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Genetic diversity of bovine viral diarrhoea virus (BVDV) from Peru and Chile

et al. 2009

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“…Despite the growing interest in the molecular epidemiology of pestiviruses [9][10][11][12][13][14][15][16], there have been few attempts to determine which regions of the pestivirus genome are subject to positive selection, although this may be a key indicator of the nature of the interaction between host and virus.…”

Section: Introductionmentioning

confidence: 99%

Evidence for positive selection on the E2 gene of bovine viral diarrhoea virus type 1

Tang

Zhang

2007

Virus Genes

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Despite the growing interest in the molecular epidemiology of pestivirus, there have been few attempts to determine which regions of the pestivirus genome are subject to positive selection, although this may be a key indicator of the nature of the interaction between host and virus. By using likelihood-based methods for phylogenetic inference, the positive selection pressure of BVDV-1 E2 gene were assessed and a site-by-site analysis of the dN/dS ratio was performed, to identify specific codons undergoing diversifying positive selection. The overall omega was 0.20, indicating that most sites were subject to strong purifying selection and five positively selected sites (886, 888, 905, 944, and 946) were identified. It is surprising to find that all the potential positively selected sites fall within the C-terminal of E2, and out of the N-terminal of E2 which is thought to be surface-exposed and therefore prime targets for host antibody response. In conclusion, these results suggest that selection favoring avoidance of antibody recognition has not been a major factor in the history of BVDV-1. Further analysis is necessary to see if amino acid substitutions in the BVDV-1 positively selected sites can lead to change of host tropism or\and escape from epitope-specific CD8 T-cell response.

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“…In the case of BVDV-2 species, the highest occurrence is reported in the USA and Canada (Pellerin et al, 1994;Ridpath et al, 1994), partially in Japan (Nagai et al, 2001), South America (Flores et al, 2002) and occasionally in some European countries (Wolfmeyer et al, 1997;Vilcek et al, 2002Vilcek et al, , 2003Pizarro-Lucero et al, 2006). No thorough genetic typing of the BVDV subtypes occurring in the Czech Republic has been done yet.…”

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confidence: 99%