Molecular characterization of Phaseolus vulgaris L. genotypes included in Bulgarian collection by ISSR and AFLP™ analyses

Svetleva, D.; Pereira, Graça; Carlier, Jorge Dias; Cabrita, Luís; Leitão, José; Genchev, D.

doi:10.1016/j.scienta.2006.04.001

Cited by 47 publications

(43 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pair-wise similarities ranged from 0.47 (SA-34 vs. PI-Agro-07) to 0.84 (SA-19 vs. SA-21). An average similarity coefficient of 0.65 was found, which was higher than the average similarity coefficient (0.52) of Kumar et al (2008), but lower than the average similarity coefficient (0.91) reported by Svetleva et al (2006).…”

Section: Resultscontrasting

confidence: 66%

“…The average PIC i value for the amplification products, across eight primer pairs, was 0.2065. Kumar et al (2008) and Svetleva et al (2006) used the AFLP technique, each with different primer pairs, to analyze the genetic diversity of common bean varieties and landraces collected at different locations, and found an average similarity coefficient of 0.52 and 0.91, Genetic diversity in NaN 3 -induced common bean mutants and commercial varieties detected by AFLP respectively. However, only very limited AFLP data are available for evaluating the genetic diversity in crop mutants (Lu et al 2007, Rashid et al 2009) obtained by induced mutation.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity in NaN3-induced common bean mutants and commercial varieties detected by AFLP

Jeng

et al. 2011

Crop Breed. Appl. Biotechnol.

View full text Add to dashboard Cite

show abstract

Section: Resultscontrasting

confidence: 66%

Section: Resultsmentioning

confidence: 99%

Genetic diversity in NaN3-induced common bean mutants and commercial varieties detected by AFLP

Jeng

et al. 2011

Crop Breed. Appl. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Besides being as low-cost as RAPD, ISSR markers are highly recommended due to its great reproducibility and ease to be applied in genetic improvement programs (WEISING et al, 2005). Even with a considerably lower number of amplified fragments, the ISSR primers accessed a greater proportion of polymorphic characters than AFLP, as previously reported for common bean (SVETLEVA et al, 2006). However, the opposite situation generally occurs, as reported for Tribulus terrestris L. (SARWAT; DAS; SRIVASTAVA, 2008).…”

Section: Resultssupporting

confidence: 56%

Accessing genetic diversity levels of Brazilian genotypes of castor with AFLP and ISSR markers

Vasconcelos

Onofre

Milani

et al. 2016

PAP

View full text Add to dashboard Cite

Castor (Ricinus communis L.), an important oilseed crop for the production of biofuels and high quality lubricants, has a long history of cultivation worldwide. However, very little is known about the genetic diversity of cultivars adapted to the semi-arid conditions of Northeastern Brazil, a strategic area for castor cultivation. Aiming to provide new approaches to select dominant markers for genetic diversity analysis among castor accessions from a Brazilian germplasm bank, we have tested 21 AFLP primer combinations and 16 ISSR primers with 27 genotypes from a Brazilian germplasm bank. PIC, MI and RP values were calculated for both marker systems and a strong correlation was observed among these parameters. Therefore, due to a higher degree of variation, primers could be better selected taking into account the RP values, which ranged from 0.231 to 18.231 (AFLP) and from 1.630 to 9.556 (ISSR). Dissimilarity values ranged from 0.189 to 0.504 in the combined analysis (AFLP + ISSR), with genotypes grouping into four main clusters, although without any evidence of genetic structuration (F st = 0.108). The successful association between genotypes and specific marks highlighted the great potential of AFLP and ISSR markers to access the variability within the species. Additionally, by describing new polymorphic markers for genotype differentiation, we provide an important contribution to guide crosses between accessions with traits of interest. KEYWORDS:Ricinus communis, DNA fingerprinting, dominant markers, germplasm. RESUMO:Visando descrever novas abordagens para a seleção de marcadores dominantes para análises de diversidade genética entre acessos de mamona, foram aplicadas 21 combinações de marcadores AFLP e 16 marcadores ISSR utilizando 27 genótipos de um banco de germoplasma brasileiro. Valores de PIC, MI e RP foram calculados para ambos os sistemas de marcadores e foi observada uma forte correlação entre tais parâmetros. Assim, devido a um maior grau de variação, os primers puderam ser melhor selecionados por meio dos valores de RP, os quais foram observados entre 0,231 e 18,231 (AFLP) e entre 1,630 e 9,556 (ISSR). Os valores de dissimilaridade variaram entre 0,189 a 0,504 na análise combinada (AFLP + ISSR), onde os genótipos foram divididos em quatro grupos principais, embora sem haver evidência de estruturação genética (Fst = 0,108). A associação bem sucedida entre genótipos e marcas específicas evidenciou o grande potencial dos marcadores AFLP e ISSR para acessar a variabilidade dentro da espécie. Adicionalmente, por meio da descrição de novos marcadores polimórficos para a diferenciação entre genótipos, o presente trabalho traz uma grande contribuição para o direcionamento de cruzamentos entre acessos com características de interesse. we have also tested some parameters aiming for a better primer selection in future evaluations. PALAVRAS-CHAVE: Materials and MethodsThe analyzed material was provided by Embrapa Algodão (Campina Grande, Brazil) and consisted of 27 castor genotypes (Table 1) adapted ...

show abstract

“…Once DNA has beens isolated, it can be analyzed either by fragmentation by enzymes, involving restriction fragment length polymorphism (RFLP -Restriction Fragment Length Polymorphism), or by amplification (PCR-based techniques) and DNA sequencing. For example, in studies using Phaseolus, based on the use of Randomly Amplified Polymorphic DNA -RAPD (Nienhuis et al, 1995;Fofana et al, 1997), Inter-Simple Sequence Repeats -ISSR, Amplified Fragment Length Polymorphism -AFLP (Svetleva et al, 2006), diversity is calculated not only according to geographical origin and botanical status, but also depending on the cultivated group. Based on the organization of genetic diversity observed in P. lunatus, Fofana et al (1997) offer a hypothesis on the process of domestication of this species.…”

Section: Dna Molecular Markersmentioning

confidence: 99%

Genetic Diversity of Bulgarian Phaseolus vulgaris L. Germplasm Collection Through Phaseolin and Isozyme Markers

Tomlekova¹

2012

The Molecular Basis of Plant Genetic Diversity

View full text Add to dashboard Cite

Molecular characterization of Phaseolus vulgaris L. genotypes included in Bulgarian collection by ISSR and AFLP™ analyses

Cited by 47 publications

References 34 publications

Genetic diversity in NaN3-induced common bean mutants and commercial varieties detected by AFLP

Genetic diversity in NaN3-induced common bean mutants and commercial varieties detected by AFLP

Accessing genetic diversity levels of Brazilian genotypes of castor with AFLP and ISSR markers

Genetic Diversity of Bulgarian Phaseolus vulgaris L. Germplasm Collection Through Phaseolin and Isozyme Markers

Contact Info

Product

Resources

About